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HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis
Plant K(+) uptake typically consists low—affinity mechanisms mediated by Shaker K(+) channels (AKT/KAT/KC) and high‐affinity mechanisms regulated by HAK/KUP/KT transporters, which are extensively studied. However, the evolutionary and genetic roles of both K(+) uptake mechanisms for drought toleranc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336388/ https://www.ncbi.nlm.nih.gov/pubmed/31917885 http://dx.doi.org/10.1111/pbi.13332 |
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author | Feng, Xue Liu, Wenxing Qiu, Cheng‐Wei Zeng, Fanrong Wang, Yizhou Zhang, Guoping Chen, Zhong‐Hua Wu, Feibo |
author_facet | Feng, Xue Liu, Wenxing Qiu, Cheng‐Wei Zeng, Fanrong Wang, Yizhou Zhang, Guoping Chen, Zhong‐Hua Wu, Feibo |
author_sort | Feng, Xue |
collection | PubMed |
description | Plant K(+) uptake typically consists low—affinity mechanisms mediated by Shaker K(+) channels (AKT/KAT/KC) and high‐affinity mechanisms regulated by HAK/KUP/KT transporters, which are extensively studied. However, the evolutionary and genetic roles of both K(+) uptake mechanisms for drought tolerance are not fully explored in crops adapted to dryland agriculture. Here, we employed evolutionary bioinformatics, biotechnological and electrophysiological approaches to determine the role of two important K(+) transporters HvAKT2 and HvHAK1 in drought tolerance in barley. HvAKT2 and HvHAK1 were cloned and functionally characterized using barley stripe mosaic virus‐induced gene silencing (BSMV‐VIGS) in drought‐tolerant wild barley XZ5 and agrobacterium‐mediated gene transfer in the barley cultivar Golden Promise. The hallmarks of the K(+) selective filters of AKT2 and HAK1 are both found in homologues from strepotophyte algae, and they are evolutionarily conserved in strepotophyte algae and land plants. HvAKT2 and HvHAK1 are both localized to the plasma membrane and have high selectivity to K(+) and Rb(+) over other tested cations. Overexpression of HvAKT2 and HvHAK1 enhanced K(+) uptake and H(+) homoeostasis leading to drought tolerance in these transgenic lines. Moreover, HvAKT2‐ and HvHAK1‐overexpressing lines showed distinct response of K(+), H(+) and Ca(2+) fluxes across plasma membrane and production of nitric oxide and hydrogen peroxide in leaves as compared to the wild type and silenced lines. High‐ and low‐affinity K(+) uptake mechanisms and their coordination with H(+) homoeostasis play essential roles in drought adaptation of wild barley. These findings can potentially facilitate future breeding programs for resilient cereal crops in a changing global climate. |
format | Online Article Text |
id | pubmed-7336388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73363882020-07-08 HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis Feng, Xue Liu, Wenxing Qiu, Cheng‐Wei Zeng, Fanrong Wang, Yizhou Zhang, Guoping Chen, Zhong‐Hua Wu, Feibo Plant Biotechnol J Research Articles Plant K(+) uptake typically consists low—affinity mechanisms mediated by Shaker K(+) channels (AKT/KAT/KC) and high‐affinity mechanisms regulated by HAK/KUP/KT transporters, which are extensively studied. However, the evolutionary and genetic roles of both K(+) uptake mechanisms for drought tolerance are not fully explored in crops adapted to dryland agriculture. Here, we employed evolutionary bioinformatics, biotechnological and electrophysiological approaches to determine the role of two important K(+) transporters HvAKT2 and HvHAK1 in drought tolerance in barley. HvAKT2 and HvHAK1 were cloned and functionally characterized using barley stripe mosaic virus‐induced gene silencing (BSMV‐VIGS) in drought‐tolerant wild barley XZ5 and agrobacterium‐mediated gene transfer in the barley cultivar Golden Promise. The hallmarks of the K(+) selective filters of AKT2 and HAK1 are both found in homologues from strepotophyte algae, and they are evolutionarily conserved in strepotophyte algae and land plants. HvAKT2 and HvHAK1 are both localized to the plasma membrane and have high selectivity to K(+) and Rb(+) over other tested cations. Overexpression of HvAKT2 and HvHAK1 enhanced K(+) uptake and H(+) homoeostasis leading to drought tolerance in these transgenic lines. Moreover, HvAKT2‐ and HvHAK1‐overexpressing lines showed distinct response of K(+), H(+) and Ca(2+) fluxes across plasma membrane and production of nitric oxide and hydrogen peroxide in leaves as compared to the wild type and silenced lines. High‐ and low‐affinity K(+) uptake mechanisms and their coordination with H(+) homoeostasis play essential roles in drought adaptation of wild barley. These findings can potentially facilitate future breeding programs for resilient cereal crops in a changing global climate. John Wiley and Sons Inc. 2020-01-24 2020-08 /pmc/articles/PMC7336388/ /pubmed/31917885 http://dx.doi.org/10.1111/pbi.13332 Text en © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Feng, Xue Liu, Wenxing Qiu, Cheng‐Wei Zeng, Fanrong Wang, Yizhou Zhang, Guoping Chen, Zhong‐Hua Wu, Feibo HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis |
title | HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis |
title_full | HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis |
title_fullStr | HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis |
title_full_unstemmed | HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis |
title_short | HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H(+) homoeostasis |
title_sort | hvakt2 and hvhak1 confer drought tolerance in barley through enhanced leaf mesophyll h(+) homoeostasis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336388/ https://www.ncbi.nlm.nih.gov/pubmed/31917885 http://dx.doi.org/10.1111/pbi.13332 |
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