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Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture
BACKGROUND: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336497/ https://www.ncbi.nlm.nih.gov/pubmed/32631217 http://dx.doi.org/10.1186/s12863-020-00877-9 |
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author | Dong, Fu-shuang lv, Meng-yu Wang, Jin-ping Shi, Xue-ping Liang, Xin-xia Liu, Yong-wei Yang, Fan Zhao, He Chai, Jian-Fang Zhou, Shuo |
author_facet | Dong, Fu-shuang lv, Meng-yu Wang, Jin-ping Shi, Xue-ping Liang, Xin-xia Liu, Yong-wei Yang, Fan Zhao, He Chai, Jian-Fang Zhou, Shuo |
author_sort | Dong, Fu-shuang |
collection | PubMed |
description | BACKGROUND: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth. RESULTS: In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7373 in the leaves. In seedlings grown in the presence of AC, 9460 DEGs were upregulated and 7483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, including PLA, CYP73A, COMT, CYP84A, and 4CL, the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, including EIN3, BZR1, JAR1, JAZ, and PR1, and downregulated genes related to plant growth inhibition, including BKI1, ARR-B, DELLA, and ABF. CONCLUSIONS: Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance. |
format | Online Article Text |
id | pubmed-7336497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73364972020-07-08 Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture Dong, Fu-shuang lv, Meng-yu Wang, Jin-ping Shi, Xue-ping Liang, Xin-xia Liu, Yong-wei Yang, Fan Zhao, He Chai, Jian-Fang Zhou, Shuo BMC Genet Research Article BACKGROUND: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth. RESULTS: In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7373 in the leaves. In seedlings grown in the presence of AC, 9460 DEGs were upregulated and 7483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, including PLA, CYP73A, COMT, CYP84A, and 4CL, the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, including EIN3, BZR1, JAR1, JAZ, and PR1, and downregulated genes related to plant growth inhibition, including BKI1, ARR-B, DELLA, and ABF. CONCLUSIONS: Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance. BioMed Central 2020-07-06 /pmc/articles/PMC7336497/ /pubmed/32631217 http://dx.doi.org/10.1186/s12863-020-00877-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Dong, Fu-shuang lv, Meng-yu Wang, Jin-ping Shi, Xue-ping Liang, Xin-xia Liu, Yong-wei Yang, Fan Zhao, He Chai, Jian-Fang Zhou, Shuo Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture |
title | Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture |
title_full | Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture |
title_fullStr | Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture |
title_full_unstemmed | Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture |
title_short | Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture |
title_sort | transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336497/ https://www.ncbi.nlm.nih.gov/pubmed/32631217 http://dx.doi.org/10.1186/s12863-020-00877-9 |
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