Confounding factors from inducible systems for spatiotemporal gene expression regulation

Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, includ...

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Detalles Bibliográficos
Autores principales: Wüst, Rob C.I., Houtkooper, Riekelt H., Auwerx, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Viewpoint
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337492/
https://www.ncbi.nlm.nih.gov/pubmed/32428200
http://dx.doi.org/10.1083/jcb.202003031
Descripción
Sumario:Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, including whole-body endocrinology and disturbances in the gut microbiome and in mitochondrial and metabolic function.

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