Confounding factors from inducible systems for spatiotemporal gene expression regulation

Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, includ...

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Autores principales: Wüst, Rob C.I., Houtkooper, Riekelt H., Auwerx, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Viewpoint
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337492/
https://www.ncbi.nlm.nih.gov/pubmed/32428200
http://dx.doi.org/10.1083/jcb.202003031
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author Wüst, Rob C.I.
Houtkooper, Riekelt H.
Auwerx, Johan
author_facet Wüst, Rob C.I.
Houtkooper, Riekelt H.
Auwerx, Johan
author_sort Wüst, Rob C.I.
collection PubMed
description Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, including whole-body endocrinology and disturbances in the gut microbiome and in mitochondrial and metabolic function.
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spelling pubmed-73374922021-01-06 Confounding factors from inducible systems for spatiotemporal gene expression regulation Wüst, Rob C.I. Houtkooper, Riekelt H. Auwerx, Johan J Cell Biol Viewpoint Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, including whole-body endocrinology and disturbances in the gut microbiome and in mitochondrial and metabolic function. Rockefeller University Press 2020-05-19 /pmc/articles/PMC7337492/ /pubmed/32428200 http://dx.doi.org/10.1083/jcb.202003031 Text en © 2020 Wüst et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Viewpoint
Wüst, Rob C.I.
Houtkooper, Riekelt H.
Auwerx, Johan
Confounding factors from inducible systems for spatiotemporal gene expression regulation
title Confounding factors from inducible systems for spatiotemporal gene expression regulation
title_full Confounding factors from inducible systems for spatiotemporal gene expression regulation
title_fullStr Confounding factors from inducible systems for spatiotemporal gene expression regulation
title_full_unstemmed Confounding factors from inducible systems for spatiotemporal gene expression regulation
title_short Confounding factors from inducible systems for spatiotemporal gene expression regulation
title_sort confounding factors from inducible systems for spatiotemporal gene expression regulation
topic Viewpoint
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337492/
https://www.ncbi.nlm.nih.gov/pubmed/32428200
http://dx.doi.org/10.1083/jcb.202003031
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