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5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

A key aim in exploiting CRISPR–Cas is gRNA engineering to introduce additional functionalities, ranging from individual nucleotide changes that increase efficiency of on-target binding to the inclusion of larger functional RNA aptamers or ribonucleoproteins (RNPs). Cas9–gRNA interactions are crucial...

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Detalles Bibliográficos
Autores principales:	Mullally, Grace, van Aelst, Kara, Naqvi, Mohsin M, Diffin, Fiona M, Karvelis, Tautvydas, Gasiunas, Giedrius, Siksnys, Virginijus, Szczelkun, Mark D
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337959/ https://www.ncbi.nlm.nih.gov/pubmed/32496535 http://dx.doi.org/10.1093/nar/gkaa477

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