Cargando…

Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B

The nucleolus is a membrane-less nuclear structure that disassembles when cells undergo mitosis. During mitosis, nucleolar factors are thus released from the nucleolus and dynamically change their subcellular localization; however, their functions remain largely uncharacterised. Here, we found that...

Descripción completa

Detalles Bibliográficos
Autores principales: Fujimura, Akiko, Hayashi, Yuki, Kato, Kazashi, Kogure, Yuichiro, Kameyama, Mutsuro, Shimamoto, Haruka, Daitoku, Hiroaki, Fukamizu, Akiyoshi, Hirota, Toru, Kimura, Keiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337965/
https://www.ncbi.nlm.nih.gov/pubmed/32479628
http://dx.doi.org/10.1093/nar/gkaa449
_version_ 1783554588806217728
author Fujimura, Akiko
Hayashi, Yuki
Kato, Kazashi
Kogure, Yuichiro
Kameyama, Mutsuro
Shimamoto, Haruka
Daitoku, Hiroaki
Fukamizu, Akiyoshi
Hirota, Toru
Kimura, Keiji
author_facet Fujimura, Akiko
Hayashi, Yuki
Kato, Kazashi
Kogure, Yuichiro
Kameyama, Mutsuro
Shimamoto, Haruka
Daitoku, Hiroaki
Fukamizu, Akiyoshi
Hirota, Toru
Kimura, Keiji
author_sort Fujimura, Akiko
collection PubMed
description The nucleolus is a membrane-less nuclear structure that disassembles when cells undergo mitosis. During mitosis, nucleolar factors are thus released from the nucleolus and dynamically change their subcellular localization; however, their functions remain largely uncharacterised. Here, we found that a nucleolar factor called nucleolar protein 11 (NOL11) forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. This complex, referred to here as the NWC (NOL11-WDR43-Cirhin) complex, exists in nucleoli during interphase and translocates to the periphery of mitotic chromosomes, i.e., perichromosomal regions. During mitotic progression, both the congression of chromosomes to the metaphase plate and sister chromatid cohesion are impaired in the absence of the NWC complex, as it is required for the centromeric enrichment of Aurora B and the associating phosphorylation of histone H3 at threonine 3. These results reveal the characteristics of a novel protein complex consisting of nucleolar proteins, which is required for regulating kinetochores and centromeres to ensure faithful chromosome segregation.
format Online
Article
Text
id pubmed-7337965
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-73379652020-07-13 Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B Fujimura, Akiko Hayashi, Yuki Kato, Kazashi Kogure, Yuichiro Kameyama, Mutsuro Shimamoto, Haruka Daitoku, Hiroaki Fukamizu, Akiyoshi Hirota, Toru Kimura, Keiji Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The nucleolus is a membrane-less nuclear structure that disassembles when cells undergo mitosis. During mitosis, nucleolar factors are thus released from the nucleolus and dynamically change their subcellular localization; however, their functions remain largely uncharacterised. Here, we found that a nucleolar factor called nucleolar protein 11 (NOL11) forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. This complex, referred to here as the NWC (NOL11-WDR43-Cirhin) complex, exists in nucleoli during interphase and translocates to the periphery of mitotic chromosomes, i.e., perichromosomal regions. During mitotic progression, both the congression of chromosomes to the metaphase plate and sister chromatid cohesion are impaired in the absence of the NWC complex, as it is required for the centromeric enrichment of Aurora B and the associating phosphorylation of histone H3 at threonine 3. These results reveal the characteristics of a novel protein complex consisting of nucleolar proteins, which is required for regulating kinetochores and centromeres to ensure faithful chromosome segregation. Oxford University Press 2020-07-09 2020-06-01 /pmc/articles/PMC7337965/ /pubmed/32479628 http://dx.doi.org/10.1093/nar/gkaa449 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Gene regulation, Chromatin and Epigenetics
Fujimura, Akiko
Hayashi, Yuki
Kato, Kazashi
Kogure, Yuichiro
Kameyama, Mutsuro
Shimamoto, Haruka
Daitoku, Hiroaki
Fukamizu, Akiyoshi
Hirota, Toru
Kimura, Keiji
Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
title Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
title_full Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
title_fullStr Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
title_full_unstemmed Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
title_short Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
title_sort identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of aurora b
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337965/
https://www.ncbi.nlm.nih.gov/pubmed/32479628
http://dx.doi.org/10.1093/nar/gkaa449
work_keys_str_mv AT fujimuraakiko identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT hayashiyuki identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT katokazashi identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT kogureyuichiro identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT kameyamamutsuro identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT shimamotoharuka identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT daitokuhiroaki identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT fukamizuakiyoshi identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT hirotatoru identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab
AT kimurakeiji identificationofanovelnucleolarproteincomplexrequiredformitoticchromosomesegregationthroughcentromericaccumulationofaurorab