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Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
The nucleolus is a membrane-less nuclear structure that disassembles when cells undergo mitosis. During mitosis, nucleolar factors are thus released from the nucleolus and dynamically change their subcellular localization; however, their functions remain largely uncharacterised. Here, we found that...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337965/ https://www.ncbi.nlm.nih.gov/pubmed/32479628 http://dx.doi.org/10.1093/nar/gkaa449 |
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author | Fujimura, Akiko Hayashi, Yuki Kato, Kazashi Kogure, Yuichiro Kameyama, Mutsuro Shimamoto, Haruka Daitoku, Hiroaki Fukamizu, Akiyoshi Hirota, Toru Kimura, Keiji |
author_facet | Fujimura, Akiko Hayashi, Yuki Kato, Kazashi Kogure, Yuichiro Kameyama, Mutsuro Shimamoto, Haruka Daitoku, Hiroaki Fukamizu, Akiyoshi Hirota, Toru Kimura, Keiji |
author_sort | Fujimura, Akiko |
collection | PubMed |
description | The nucleolus is a membrane-less nuclear structure that disassembles when cells undergo mitosis. During mitosis, nucleolar factors are thus released from the nucleolus and dynamically change their subcellular localization; however, their functions remain largely uncharacterised. Here, we found that a nucleolar factor called nucleolar protein 11 (NOL11) forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. This complex, referred to here as the NWC (NOL11-WDR43-Cirhin) complex, exists in nucleoli during interphase and translocates to the periphery of mitotic chromosomes, i.e., perichromosomal regions. During mitotic progression, both the congression of chromosomes to the metaphase plate and sister chromatid cohesion are impaired in the absence of the NWC complex, as it is required for the centromeric enrichment of Aurora B and the associating phosphorylation of histone H3 at threonine 3. These results reveal the characteristics of a novel protein complex consisting of nucleolar proteins, which is required for regulating kinetochores and centromeres to ensure faithful chromosome segregation. |
format | Online Article Text |
id | pubmed-7337965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-73379652020-07-13 Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B Fujimura, Akiko Hayashi, Yuki Kato, Kazashi Kogure, Yuichiro Kameyama, Mutsuro Shimamoto, Haruka Daitoku, Hiroaki Fukamizu, Akiyoshi Hirota, Toru Kimura, Keiji Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The nucleolus is a membrane-less nuclear structure that disassembles when cells undergo mitosis. During mitosis, nucleolar factors are thus released from the nucleolus and dynamically change their subcellular localization; however, their functions remain largely uncharacterised. Here, we found that a nucleolar factor called nucleolar protein 11 (NOL11) forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. This complex, referred to here as the NWC (NOL11-WDR43-Cirhin) complex, exists in nucleoli during interphase and translocates to the periphery of mitotic chromosomes, i.e., perichromosomal regions. During mitotic progression, both the congression of chromosomes to the metaphase plate and sister chromatid cohesion are impaired in the absence of the NWC complex, as it is required for the centromeric enrichment of Aurora B and the associating phosphorylation of histone H3 at threonine 3. These results reveal the characteristics of a novel protein complex consisting of nucleolar proteins, which is required for regulating kinetochores and centromeres to ensure faithful chromosome segregation. Oxford University Press 2020-07-09 2020-06-01 /pmc/articles/PMC7337965/ /pubmed/32479628 http://dx.doi.org/10.1093/nar/gkaa449 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Gene regulation, Chromatin and Epigenetics Fujimura, Akiko Hayashi, Yuki Kato, Kazashi Kogure, Yuichiro Kameyama, Mutsuro Shimamoto, Haruka Daitoku, Hiroaki Fukamizu, Akiyoshi Hirota, Toru Kimura, Keiji Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B |
title | Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B |
title_full | Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B |
title_fullStr | Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B |
title_full_unstemmed | Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B |
title_short | Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B |
title_sort | identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of aurora b |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337965/ https://www.ncbi.nlm.nih.gov/pubmed/32479628 http://dx.doi.org/10.1093/nar/gkaa449 |
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