Cargando…

Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration

Numerous tissue-engineered constructs have been investigated as bone scaffolds in regenerative medicine. However, it remains challenging to non-invasively monitor the biodegradation and remodeling of bone grafts after implantation. Herein, silk fibroin/hydroxyapatite scaffolds incorporated with ultr...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Qin, Feng, Longbao, Chen, Zelong, Lan, Yong, Liu, Yu, Li, Dan, Yan, Chenggong, Xu, Yikai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338306/
https://www.ncbi.nlm.nih.gov/pubmed/32695767
http://dx.doi.org/10.3389/fbioe.2020.00697
_version_ 1783554647064051712
author Liu, Qin
Feng, Longbao
Chen, Zelong
Lan, Yong
Liu, Yu
Li, Dan
Yan, Chenggong
Xu, Yikai
author_facet Liu, Qin
Feng, Longbao
Chen, Zelong
Lan, Yong
Liu, Yu
Li, Dan
Yan, Chenggong
Xu, Yikai
author_sort Liu, Qin
collection PubMed
description Numerous tissue-engineered constructs have been investigated as bone scaffolds in regenerative medicine. However, it remains challenging to non-invasively monitor the biodegradation and remodeling of bone grafts after implantation. Herein, silk fibroin/hydroxyapatite scaffolds incorporated with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles were successfully synthesized, characterized, and implanted subcutaneously into the back of nude mice. The USPIO labeled scaffolds showed good three-dimensional porous structures and mechanical property, thermal stability for bone repair. After loaded with bone marrow-derived mesenchymal stem cells (BMSCs), the multifunctional scaffolds promoted cell adhesion and growth, and facilitated osteogenesis by showing increased levels of alkaline phosphatase activity and up-regulation of osteoblastic genes. Furthermore, in vivo quantitative magnetic resonance imaging (MRI) results provided valuable information on scaffolds degradation and bone formation simultaneously, which was further confirmed by computed tomography and histological examination. These findings demonstrated that the incorporation of USPIO into BMSCs-loaded multifunctional scaffold system could be feasible to noninvasively monitor bone regeneration by quantitative MRI. This tissue engineering strategy provides a promising tool for translational application of bone defect repair in clinical scenarios.
format Online
Article
Text
id pubmed-7338306
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-73383062020-07-20 Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration Liu, Qin Feng, Longbao Chen, Zelong Lan, Yong Liu, Yu Li, Dan Yan, Chenggong Xu, Yikai Front Bioeng Biotechnol Bioengineering and Biotechnology Numerous tissue-engineered constructs have been investigated as bone scaffolds in regenerative medicine. However, it remains challenging to non-invasively monitor the biodegradation and remodeling of bone grafts after implantation. Herein, silk fibroin/hydroxyapatite scaffolds incorporated with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles were successfully synthesized, characterized, and implanted subcutaneously into the back of nude mice. The USPIO labeled scaffolds showed good three-dimensional porous structures and mechanical property, thermal stability for bone repair. After loaded with bone marrow-derived mesenchymal stem cells (BMSCs), the multifunctional scaffolds promoted cell adhesion and growth, and facilitated osteogenesis by showing increased levels of alkaline phosphatase activity and up-regulation of osteoblastic genes. Furthermore, in vivo quantitative magnetic resonance imaging (MRI) results provided valuable information on scaffolds degradation and bone formation simultaneously, which was further confirmed by computed tomography and histological examination. These findings demonstrated that the incorporation of USPIO into BMSCs-loaded multifunctional scaffold system could be feasible to noninvasively monitor bone regeneration by quantitative MRI. This tissue engineering strategy provides a promising tool for translational application of bone defect repair in clinical scenarios. Frontiers Media S.A. 2020-06-30 /pmc/articles/PMC7338306/ /pubmed/32695767 http://dx.doi.org/10.3389/fbioe.2020.00697 Text en Copyright © 2020 Liu, Feng, Chen, Lan, Liu, Li, Yan and Xu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liu, Qin
Feng, Longbao
Chen, Zelong
Lan, Yong
Liu, Yu
Li, Dan
Yan, Chenggong
Xu, Yikai
Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
title Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
title_full Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
title_fullStr Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
title_full_unstemmed Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
title_short Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
title_sort ultrasmall superparamagnetic iron oxide labeled silk fibroin/hydroxyapatite multifunctional scaffold loaded with bone marrow-derived mesenchymal stem cells for bone regeneration
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338306/
https://www.ncbi.nlm.nih.gov/pubmed/32695767
http://dx.doi.org/10.3389/fbioe.2020.00697
work_keys_str_mv AT liuqin ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration
AT fenglongbao ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration
AT chenzelong ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration
AT lanyong ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration
AT liuyu ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration
AT lidan ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration
AT yanchenggong ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration
AT xuyikai ultrasmallsuperparamagneticironoxidelabeledsilkfibroinhydroxyapatitemultifunctionalscaffoldloadedwithbonemarrowderivedmesenchymalstemcellsforboneregeneration