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The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)

Trichomoniasis is caused by Trichomonas vaginalis (T. vaginalis), which is a widespread and serious sexually transmitted pathogen in humans. The procedure of T. vaginalis adherence to the host cell is the precondition for T. vaginalis parasitism and pathogenicity. The AP33 adhesin of T. vaginalis (T...

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Autores principales: Zhang, Zhenchao, Li, Yuhua, Wang, Shuai, Hao, Lixia, Zhu, Yunqing, Li, Haoran, Song, Xiaoxiao, Duan, Yujuan, Sang, Yuhui, Wu, Pucheng, Li, Xiangrui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338309/
https://www.ncbi.nlm.nih.gov/pubmed/32695085
http://dx.doi.org/10.3389/fmicb.2020.01433
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author Zhang, Zhenchao
Li, Yuhua
Wang, Shuai
Hao, Lixia
Zhu, Yunqing
Li, Haoran
Song, Xiaoxiao
Duan, Yujuan
Sang, Yuhui
Wu, Pucheng
Li, Xiangrui
author_facet Zhang, Zhenchao
Li, Yuhua
Wang, Shuai
Hao, Lixia
Zhu, Yunqing
Li, Haoran
Song, Xiaoxiao
Duan, Yujuan
Sang, Yuhui
Wu, Pucheng
Li, Xiangrui
author_sort Zhang, Zhenchao
collection PubMed
description Trichomoniasis is caused by Trichomonas vaginalis (T. vaginalis), which is a widespread and serious sexually transmitted pathogen in humans. The procedure of T. vaginalis adherence to the host cell is the precondition for T. vaginalis parasitism and pathogenicity. The AP33 adhesin of T. vaginalis (TvAP33) plays a key role in the process of adhesion. In this study, the specific primers for polymerase chain reaction (PCR) were designed based on the sequence of TvAP33 (GenBank Accession No. U87098.1) to amplify the open reading frame (ORF), and the ORF was inserted into pET-32a (+) to produce recombinant TvAP33 (rTvAP33). The sequence analysis indicated that the TvAP33 gene encoded a protein of 309 amino acids with 32.53 kDa, and the protein was predicted to have a high antigen index. Western blotting assay showed rTvAP33 was successfully recognized by the sera of mice experimentally infected with T. vaginalis, while native TvAP33 in the somatic extract of T. vaginalis trophozoite was as well detected by sera from rats immunized with the rTvAP33. Immunofluorescence analysis using an antibody against rTvAP33 demonstrated that the protein was expressed and located on the surface of T. vaginalis trophozoites. The recombinant protein was emulsified in Freund’s adjuvant and used to immunize BALB/C mice three times at days 0, 14, and 28. The result of animal challenge experiments revealed the levels of IgG, IgG1, and IgG2a, and IL-4, IL-10, and IL17 among rTvAP33 vaccinated animals were integrally increased. Moreover, the rTvAP33 vaccinated animals were apparently prolonged survival time (26.45 ± 4.10) after challenge infection with this parasite. All these results indicated that TvAP33 could be used as vaccine candidate antigen to induce cell-mediated and humoral immunity.
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spelling pubmed-73383092020-07-20 The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33) Zhang, Zhenchao Li, Yuhua Wang, Shuai Hao, Lixia Zhu, Yunqing Li, Haoran Song, Xiaoxiao Duan, Yujuan Sang, Yuhui Wu, Pucheng Li, Xiangrui Front Microbiol Microbiology Trichomoniasis is caused by Trichomonas vaginalis (T. vaginalis), which is a widespread and serious sexually transmitted pathogen in humans. The procedure of T. vaginalis adherence to the host cell is the precondition for T. vaginalis parasitism and pathogenicity. The AP33 adhesin of T. vaginalis (TvAP33) plays a key role in the process of adhesion. In this study, the specific primers for polymerase chain reaction (PCR) were designed based on the sequence of TvAP33 (GenBank Accession No. U87098.1) to amplify the open reading frame (ORF), and the ORF was inserted into pET-32a (+) to produce recombinant TvAP33 (rTvAP33). The sequence analysis indicated that the TvAP33 gene encoded a protein of 309 amino acids with 32.53 kDa, and the protein was predicted to have a high antigen index. Western blotting assay showed rTvAP33 was successfully recognized by the sera of mice experimentally infected with T. vaginalis, while native TvAP33 in the somatic extract of T. vaginalis trophozoite was as well detected by sera from rats immunized with the rTvAP33. Immunofluorescence analysis using an antibody against rTvAP33 demonstrated that the protein was expressed and located on the surface of T. vaginalis trophozoites. The recombinant protein was emulsified in Freund’s adjuvant and used to immunize BALB/C mice three times at days 0, 14, and 28. The result of animal challenge experiments revealed the levels of IgG, IgG1, and IgG2a, and IL-4, IL-10, and IL17 among rTvAP33 vaccinated animals were integrally increased. Moreover, the rTvAP33 vaccinated animals were apparently prolonged survival time (26.45 ± 4.10) after challenge infection with this parasite. All these results indicated that TvAP33 could be used as vaccine candidate antigen to induce cell-mediated and humoral immunity. Frontiers Media S.A. 2020-06-30 /pmc/articles/PMC7338309/ /pubmed/32695085 http://dx.doi.org/10.3389/fmicb.2020.01433 Text en Copyright © 2020 Zhang, Li, Wang, Hao, Zhu, Li, Song, Duan, Sang, Wu and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Zhenchao
Li, Yuhua
Wang, Shuai
Hao, Lixia
Zhu, Yunqing
Li, Haoran
Song, Xiaoxiao
Duan, Yujuan
Sang, Yuhui
Wu, Pucheng
Li, Xiangrui
The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
title The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
title_full The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
title_fullStr The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
title_full_unstemmed The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
title_short The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
title_sort molecular characterization and immunity identification of trichomonas vaginalis adhesion protein 33 (ap33)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338309/
https://www.ncbi.nlm.nih.gov/pubmed/32695085
http://dx.doi.org/10.3389/fmicb.2020.01433
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