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New trends in single-molecule bioanalytical detection
Single-molecule sensing is becoming a major driver in biomarker assays as it is foreseen to enable precision medicine to enter into everyday clinical practice. However, among the single-molecule detection methods proposed so far, only a few are fully exploitable for the ultrasensitive label-free ass...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338812/ https://www.ncbi.nlm.nih.gov/pubmed/32185439 http://dx.doi.org/10.1007/s00216-020-02540-9 |
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author | Macchia, Eleonora Manoli, Kyriaki Di Franco, Cincia Scamarcio, Gaetano Torsi, Luisa |
author_facet | Macchia, Eleonora Manoli, Kyriaki Di Franco, Cincia Scamarcio, Gaetano Torsi, Luisa |
author_sort | Macchia, Eleonora |
collection | PubMed |
description | Single-molecule sensing is becoming a major driver in biomarker assays as it is foreseen to enable precision medicine to enter into everyday clinical practice. However, among the single-molecule detection methods proposed so far, only a few are fully exploitable for the ultrasensitive label-free assay of biofluids. Firstly introduced single-molecule sensing platforms encompass low-background-noise fluorescent microscopy as well as plasmonic and electrical nanotransducers; these are generally able to sense at the nanomolar concentration level or higher. Label-based single-molecule technologies relying on optical transduction and microbeads that can scavenge and detect a few biomarkers in the bulk of real biofluids, reaching ultralow detection limits, have been recently commercialized. These assays, thanks to the extremely high sensitivity and convenient handling, are new trends in the field as they are paving the way to a revolution in early diagnostics. Very recently, another new trend is the label-free, organic bioelectronic electrolyte-gated large transistors that can potentially be produced by means of large-area low-cost technologies and have been proven capable to detect a protein at the physical limit in real bovine serum. This article offers a bird’s-eye view on some of the more significant single-molecule bioanalytical technologies and highlights their sensing principles and figures-of-merit such as limit of detection, need for a labelling step, and possibility to operate, also as an array, directly in real biofluids. We also discuss the new trend towards single-molecule proof-of-principle extremely sensitive technologies that can detect a protein at the zeptomolar concentration level involving label-free devices that potentially offer low-cost production and easy scalability. |
format | Online Article Text |
id | pubmed-7338812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-73388122020-07-09 New trends in single-molecule bioanalytical detection Macchia, Eleonora Manoli, Kyriaki Di Franco, Cincia Scamarcio, Gaetano Torsi, Luisa Anal Bioanal Chem Trends Single-molecule sensing is becoming a major driver in biomarker assays as it is foreseen to enable precision medicine to enter into everyday clinical practice. However, among the single-molecule detection methods proposed so far, only a few are fully exploitable for the ultrasensitive label-free assay of biofluids. Firstly introduced single-molecule sensing platforms encompass low-background-noise fluorescent microscopy as well as plasmonic and electrical nanotransducers; these are generally able to sense at the nanomolar concentration level or higher. Label-based single-molecule technologies relying on optical transduction and microbeads that can scavenge and detect a few biomarkers in the bulk of real biofluids, reaching ultralow detection limits, have been recently commercialized. These assays, thanks to the extremely high sensitivity and convenient handling, are new trends in the field as they are paving the way to a revolution in early diagnostics. Very recently, another new trend is the label-free, organic bioelectronic electrolyte-gated large transistors that can potentially be produced by means of large-area low-cost technologies and have been proven capable to detect a protein at the physical limit in real bovine serum. This article offers a bird’s-eye view on some of the more significant single-molecule bioanalytical technologies and highlights their sensing principles and figures-of-merit such as limit of detection, need for a labelling step, and possibility to operate, also as an array, directly in real biofluids. We also discuss the new trend towards single-molecule proof-of-principle extremely sensitive technologies that can detect a protein at the zeptomolar concentration level involving label-free devices that potentially offer low-cost production and easy scalability. Springer Berlin Heidelberg 2020-03-17 2020 /pmc/articles/PMC7338812/ /pubmed/32185439 http://dx.doi.org/10.1007/s00216-020-02540-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Trends Macchia, Eleonora Manoli, Kyriaki Di Franco, Cincia Scamarcio, Gaetano Torsi, Luisa New trends in single-molecule bioanalytical detection |
title | New trends in single-molecule bioanalytical detection |
title_full | New trends in single-molecule bioanalytical detection |
title_fullStr | New trends in single-molecule bioanalytical detection |
title_full_unstemmed | New trends in single-molecule bioanalytical detection |
title_short | New trends in single-molecule bioanalytical detection |
title_sort | new trends in single-molecule bioanalytical detection |
topic | Trends |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338812/ https://www.ncbi.nlm.nih.gov/pubmed/32185439 http://dx.doi.org/10.1007/s00216-020-02540-9 |
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