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Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture

Abnormal microRNA (miRNA) expression has been implicated in spinal cord injury (SCI), but the underlying mechanisms are poorly understood. To observe the effect of electroacupuncture (EA) on miRNA expression profiles in SCI rats and investigate the potential mechanisms involved in this process, Spra...

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Autores principales: Zhou, Zhidong, Li, Hejian, Li, Hongchun, Zhang, Jing, Fu, Kaiwen, Cao, Cao, Deng, Fumou, Luo, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339738/
https://www.ncbi.nlm.nih.gov/pubmed/32468009
http://dx.doi.org/10.3892/mmr.2020.11161
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author Zhou, Zhidong
Li, Hejian
Li, Hongchun
Zhang, Jing
Fu, Kaiwen
Cao, Cao
Deng, Fumou
Luo, Jun
author_facet Zhou, Zhidong
Li, Hejian
Li, Hongchun
Zhang, Jing
Fu, Kaiwen
Cao, Cao
Deng, Fumou
Luo, Jun
author_sort Zhou, Zhidong
collection PubMed
description Abnormal microRNA (miRNA) expression has been implicated in spinal cord injury (SCI), but the underlying mechanisms are poorly understood. To observe the effect of electroacupuncture (EA) on miRNA expression profiles in SCI rats and investigate the potential mechanisms involved in this process, Sprague-Dawley rats were divided into sham, SCI and SCI+EA groups (n=6 each). Basso, Beattie and Bresnahan (BBB) scoring and hematoxylin-eosin staining of cortical tissues were used to evaluate spinal cord recovery with EA treatment 21 days post-surgery across the three groups. To investigate miRNA expression profiles, 6 Sprague-Dawley rats were randomly divided into SCI and SCI+EA groups (n=3 in each group) and examined using next-generation sequencing. Integrated miRNA-mRNA-pathway network analysis was performed to elucidate the interaction network of the candidate miRNAs, their target genes and the involved pathways. Behavioral scores suggested that hindlimb motor functions improved with EA treatments. Apoptotic indices were lower in the SCI+EA group compared with the SCI group. It was also observed that 168 miRNAs were differentially expressed between the SCI and SCI+EA groups, with 29 upregulated and 139 downregulated miRNAs in the SCI+EA group. Changes in miRNA expression are involved in SCI physiopathology, including inflammation and apoptosis. Reverse transcription-quantitative PCR measurement of the five candidate miRNAs, namely rno-miR-219a-5p, rno-miR-486, rno-miR-136-5p, rno-miR-128-3p, and rno-miR-7b, was consistent with RNA sequencing data. Integrated miRNA-mRNA-pathway analysis suggested that the MAPK, Wnt and NF-κB signaling pathways were involved in EA-mediated recovery from SCI. The present study evaluated the miRNA expression profiles involved in EA-treated SCI rats and demonstrated the potential mechanism and functional role of miRNAs in SCI in rats.
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spelling pubmed-73397382020-07-09 Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture Zhou, Zhidong Li, Hejian Li, Hongchun Zhang, Jing Fu, Kaiwen Cao, Cao Deng, Fumou Luo, Jun Mol Med Rep Articles Abnormal microRNA (miRNA) expression has been implicated in spinal cord injury (SCI), but the underlying mechanisms are poorly understood. To observe the effect of electroacupuncture (EA) on miRNA expression profiles in SCI rats and investigate the potential mechanisms involved in this process, Sprague-Dawley rats were divided into sham, SCI and SCI+EA groups (n=6 each). Basso, Beattie and Bresnahan (BBB) scoring and hematoxylin-eosin staining of cortical tissues were used to evaluate spinal cord recovery with EA treatment 21 days post-surgery across the three groups. To investigate miRNA expression profiles, 6 Sprague-Dawley rats were randomly divided into SCI and SCI+EA groups (n=3 in each group) and examined using next-generation sequencing. Integrated miRNA-mRNA-pathway network analysis was performed to elucidate the interaction network of the candidate miRNAs, their target genes and the involved pathways. Behavioral scores suggested that hindlimb motor functions improved with EA treatments. Apoptotic indices were lower in the SCI+EA group compared with the SCI group. It was also observed that 168 miRNAs were differentially expressed between the SCI and SCI+EA groups, with 29 upregulated and 139 downregulated miRNAs in the SCI+EA group. Changes in miRNA expression are involved in SCI physiopathology, including inflammation and apoptosis. Reverse transcription-quantitative PCR measurement of the five candidate miRNAs, namely rno-miR-219a-5p, rno-miR-486, rno-miR-136-5p, rno-miR-128-3p, and rno-miR-7b, was consistent with RNA sequencing data. Integrated miRNA-mRNA-pathway analysis suggested that the MAPK, Wnt and NF-κB signaling pathways were involved in EA-mediated recovery from SCI. The present study evaluated the miRNA expression profiles involved in EA-treated SCI rats and demonstrated the potential mechanism and functional role of miRNAs in SCI in rats. D.A. Spandidos 2020-08 2020-05-20 /pmc/articles/PMC7339738/ /pubmed/32468009 http://dx.doi.org/10.3892/mmr.2020.11161 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhou, Zhidong
Li, Hejian
Li, Hongchun
Zhang, Jing
Fu, Kaiwen
Cao, Cao
Deng, Fumou
Luo, Jun
Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture
title Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture
title_full Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture
title_fullStr Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture
title_full_unstemmed Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture
title_short Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture
title_sort comprehensive analysis of the differential expression profile of micrornas in rats with spinal cord injury treated by electroacupuncture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339738/
https://www.ncbi.nlm.nih.gov/pubmed/32468009
http://dx.doi.org/10.3892/mmr.2020.11161
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