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Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2)

AIMS: Electronic (e)-cigarettes have been marketed as a ‘healthy’ alternative to traditional combustible cigarettes and as an effective method of smoking cessation. There are, however, a paucity of data to support these claims. In fact, e-cigarettes are implicated in endothelial dysfunction and oxid...

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Autores principales: Kuntic, Marin, Oelze, Matthias, Steven, Sebastian, Kröller-Schön, Swenja, Stamm, Paul, Kalinovic, Sanela, Frenis, Katie, Vujacic-Mirski, Ksenija, Bayo Jimenez, Maria Teresa, Kvandova, Miroslava, Filippou, Konstantina, Al Zuabi, Ahmad, Brückl, Vivienne, Hahad, Omar, Daub, Steffen, Varveri, Franco, Gori, Tommaso, Huesmann, Regina, Hoffmann, Thorsten, Schmidt, Frank P, Keaney, John F, Daiber, Andreas, Münzel, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340357/
https://www.ncbi.nlm.nih.gov/pubmed/31715629
http://dx.doi.org/10.1093/eurheartj/ehz772
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author Kuntic, Marin
Oelze, Matthias
Steven, Sebastian
Kröller-Schön, Swenja
Stamm, Paul
Kalinovic, Sanela
Frenis, Katie
Vujacic-Mirski, Ksenija
Bayo Jimenez, Maria Teresa
Kvandova, Miroslava
Filippou, Konstantina
Al Zuabi, Ahmad
Brückl, Vivienne
Hahad, Omar
Daub, Steffen
Varveri, Franco
Gori, Tommaso
Huesmann, Regina
Hoffmann, Thorsten
Schmidt, Frank P
Keaney, John F
Daiber, Andreas
Münzel, Thomas
author_facet Kuntic, Marin
Oelze, Matthias
Steven, Sebastian
Kröller-Schön, Swenja
Stamm, Paul
Kalinovic, Sanela
Frenis, Katie
Vujacic-Mirski, Ksenija
Bayo Jimenez, Maria Teresa
Kvandova, Miroslava
Filippou, Konstantina
Al Zuabi, Ahmad
Brückl, Vivienne
Hahad, Omar
Daub, Steffen
Varveri, Franco
Gori, Tommaso
Huesmann, Regina
Hoffmann, Thorsten
Schmidt, Frank P
Keaney, John F
Daiber, Andreas
Münzel, Thomas
author_sort Kuntic, Marin
collection PubMed
description AIMS: Electronic (e)-cigarettes have been marketed as a ‘healthy’ alternative to traditional combustible cigarettes and as an effective method of smoking cessation. There are, however, a paucity of data to support these claims. In fact, e-cigarettes are implicated in endothelial dysfunction and oxidative stress in the vasculature and the lungs. The mechanisms underlying these side effects remain unclear. Here, we investigated the effects of e-cigarette vapour on vascular function in smokers and experimental animals to determine the underlying mechanisms. METHODS AND RESULTS: Acute e-cigarette smoking produced a marked impairment of endothelial function in chronic smokers determined by flow-mediated dilation. In mice, e-cigarette vapour without nicotine had more detrimental effects on endothelial function, markers of oxidative stress, inflammation, and lipid peroxidation than vapour containing nicotine. These effects of e-cigarette vapour were largely absent in mice lacking phagocytic NADPH oxidase (NOX-2) or upon treatment with the endothelin receptor blocker macitentan or the FOXO3 activator bepridil. We also established that the e-cigarette product acrolein, a reactive aldehyde, recapitulated many of the NOX-2-dependent effects of e-cigarette vapour using in vitro blood vessel incubation. CONCLUSIONS: E-cigarette vapour exposure increases vascular, cerebral, and pulmonary oxidative stress via a NOX-2-dependent mechanism. Our study identifies the toxic aldehyde acrolein as a key mediator of the observed adverse vascular consequences. Thus, e-cigarettes have the potential to induce marked adverse cardiovascular, pulmonary, and cerebrovascular consequences. Since e-cigarette use is increasing, particularly amongst youth, our data suggest that aggressive steps are warranted to limit their health risks.
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spelling pubmed-73403572020-07-13 Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2) Kuntic, Marin Oelze, Matthias Steven, Sebastian Kröller-Schön, Swenja Stamm, Paul Kalinovic, Sanela Frenis, Katie Vujacic-Mirski, Ksenija Bayo Jimenez, Maria Teresa Kvandova, Miroslava Filippou, Konstantina Al Zuabi, Ahmad Brückl, Vivienne Hahad, Omar Daub, Steffen Varveri, Franco Gori, Tommaso Huesmann, Regina Hoffmann, Thorsten Schmidt, Frank P Keaney, John F Daiber, Andreas Münzel, Thomas Eur Heart J Basic Science AIMS: Electronic (e)-cigarettes have been marketed as a ‘healthy’ alternative to traditional combustible cigarettes and as an effective method of smoking cessation. There are, however, a paucity of data to support these claims. In fact, e-cigarettes are implicated in endothelial dysfunction and oxidative stress in the vasculature and the lungs. The mechanisms underlying these side effects remain unclear. Here, we investigated the effects of e-cigarette vapour on vascular function in smokers and experimental animals to determine the underlying mechanisms. METHODS AND RESULTS: Acute e-cigarette smoking produced a marked impairment of endothelial function in chronic smokers determined by flow-mediated dilation. In mice, e-cigarette vapour without nicotine had more detrimental effects on endothelial function, markers of oxidative stress, inflammation, and lipid peroxidation than vapour containing nicotine. These effects of e-cigarette vapour were largely absent in mice lacking phagocytic NADPH oxidase (NOX-2) or upon treatment with the endothelin receptor blocker macitentan or the FOXO3 activator bepridil. We also established that the e-cigarette product acrolein, a reactive aldehyde, recapitulated many of the NOX-2-dependent effects of e-cigarette vapour using in vitro blood vessel incubation. CONCLUSIONS: E-cigarette vapour exposure increases vascular, cerebral, and pulmonary oxidative stress via a NOX-2-dependent mechanism. Our study identifies the toxic aldehyde acrolein as a key mediator of the observed adverse vascular consequences. Thus, e-cigarettes have the potential to induce marked adverse cardiovascular, pulmonary, and cerebrovascular consequences. Since e-cigarette use is increasing, particularly amongst youth, our data suggest that aggressive steps are warranted to limit their health risks. Oxford University Press 2020-07-07 2019-11-13 /pmc/articles/PMC7340357/ /pubmed/31715629 http://dx.doi.org/10.1093/eurheartj/ehz772 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Cardiology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Basic Science
Kuntic, Marin
Oelze, Matthias
Steven, Sebastian
Kröller-Schön, Swenja
Stamm, Paul
Kalinovic, Sanela
Frenis, Katie
Vujacic-Mirski, Ksenija
Bayo Jimenez, Maria Teresa
Kvandova, Miroslava
Filippou, Konstantina
Al Zuabi, Ahmad
Brückl, Vivienne
Hahad, Omar
Daub, Steffen
Varveri, Franco
Gori, Tommaso
Huesmann, Regina
Hoffmann, Thorsten
Schmidt, Frank P
Keaney, John F
Daiber, Andreas
Münzel, Thomas
Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2)
title Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2)
title_full Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2)
title_fullStr Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2)
title_full_unstemmed Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2)
title_short Short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic NADPH oxidase (NOX-2)
title_sort short-term e-cigarette vapour exposure causes vascular oxidative stress and dysfunction: evidence for a close connection to brain damage and a key role of the phagocytic nadph oxidase (nox-2)
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340357/
https://www.ncbi.nlm.nih.gov/pubmed/31715629
http://dx.doi.org/10.1093/eurheartj/ehz772
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