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Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy

Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose...

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Detalles Bibliográficos
Autores principales: Antonello, Jacopo, Barbotin, Aurélien, Chong, Ee Zhuan, Rittscher, Jens, Booth, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340381/
https://www.ncbi.nlm.nih.gov/pubmed/32549490
http://dx.doi.org/10.1364/OE.393363
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author Antonello, Jacopo
Barbotin, Aurélien
Chong, Ee Zhuan
Rittscher, Jens
Booth, Martin J.
author_facet Antonello, Jacopo
Barbotin, Aurélien
Chong, Ee Zhuan
Rittscher, Jens
Booth, Martin J.
author_sort Antonello, Jacopo
collection PubMed
description Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose using wavelet analysis to quantify the loss of resolution due to the aberrations in microscope images. By examining the variations of the wavelet coefficients at different scales, we are able to establish a multi-valued image quality metric that can be successfully deployed in different microscopy techniques. To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens.
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spelling pubmed-73403812020-09-23 Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy Antonello, Jacopo Barbotin, Aurélien Chong, Ee Zhuan Rittscher, Jens Booth, Martin J. Opt Express Article Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose using wavelet analysis to quantify the loss of resolution due to the aberrations in microscope images. By examining the variations of the wavelet coefficients at different scales, we are able to establish a multi-valued image quality metric that can be successfully deployed in different microscopy techniques. To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens. Optical Society of America 2020-05-19 /pmc/articles/PMC7340381/ /pubmed/32549490 http://dx.doi.org/10.1364/OE.393363 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
spellingShingle Article
Antonello, Jacopo
Barbotin, Aurélien
Chong, Ee Zhuan
Rittscher, Jens
Booth, Martin J.
Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
title Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
title_full Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
title_fullStr Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
title_full_unstemmed Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
title_short Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
title_sort multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340381/
https://www.ncbi.nlm.nih.gov/pubmed/32549490
http://dx.doi.org/10.1364/OE.393363
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