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Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340381/ https://www.ncbi.nlm.nih.gov/pubmed/32549490 http://dx.doi.org/10.1364/OE.393363 |
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author | Antonello, Jacopo Barbotin, Aurélien Chong, Ee Zhuan Rittscher, Jens Booth, Martin J. |
author_facet | Antonello, Jacopo Barbotin, Aurélien Chong, Ee Zhuan Rittscher, Jens Booth, Martin J. |
author_sort | Antonello, Jacopo |
collection | PubMed |
description | Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose using wavelet analysis to quantify the loss of resolution due to the aberrations in microscope images. By examining the variations of the wavelet coefficients at different scales, we are able to establish a multi-valued image quality metric that can be successfully deployed in different microscopy techniques. To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens. |
format | Online Article Text |
id | pubmed-7340381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-73403812020-09-23 Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy Antonello, Jacopo Barbotin, Aurélien Chong, Ee Zhuan Rittscher, Jens Booth, Martin J. Opt Express Article Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose using wavelet analysis to quantify the loss of resolution due to the aberrations in microscope images. By examining the variations of the wavelet coefficients at different scales, we are able to establish a multi-valued image quality metric that can be successfully deployed in different microscopy techniques. To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens. Optical Society of America 2020-05-19 /pmc/articles/PMC7340381/ /pubmed/32549490 http://dx.doi.org/10.1364/OE.393363 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. |
spellingShingle | Article Antonello, Jacopo Barbotin, Aurélien Chong, Ee Zhuan Rittscher, Jens Booth, Martin J. Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
title | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
title_full | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
title_fullStr | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
title_full_unstemmed | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
title_short | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
title_sort | multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340381/ https://www.ncbi.nlm.nih.gov/pubmed/32549490 http://dx.doi.org/10.1364/OE.393363 |
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