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Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards

BACKGROUND AND OBJECTIVES: Cytomegalovirus (CMV) constitutes the most common viral cause of congenital infections in newborns worldwide. There are a significant number of asymptomatic newborns with congenital CMV infection in Iran, which may develop long-term sequelae of infection. Unfortunately, li...

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Autores principales: Noorbakhsh, Samileh, Farhadi, Mohammad, Haghighi, Faezeh, Minaeian, Sara, Hasanabad, Morteza Haghighi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340612/
https://www.ncbi.nlm.nih.gov/pubmed/32685115
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author Noorbakhsh, Samileh
Farhadi, Mohammad
Haghighi, Faezeh
Minaeian, Sara
Hasanabad, Morteza Haghighi
author_facet Noorbakhsh, Samileh
Farhadi, Mohammad
Haghighi, Faezeh
Minaeian, Sara
Hasanabad, Morteza Haghighi
author_sort Noorbakhsh, Samileh
collection PubMed
description BACKGROUND AND OBJECTIVES: Cytomegalovirus (CMV) constitutes the most common viral cause of congenital infections in newborns worldwide. There are a significant number of asymptomatic newborns with congenital CMV infection in Iran, which may develop long-term sequelae of infection. Unfortunately, limited data exsists from Iran on the rate of congenital CMV infection among neonates. The current study was aimed to investigate the prevalence of congenital CMV infection among Iranian neonates by testing Guthrie cards. MATERIALS AND METHODS: Guthrie cards were collected from infants within 2 weeks of life, and total DNA was extracted from samples by thermal shock and evaluated for CMV DNA using nested-PCR assay. CMV infection in newborns was confirmed through a commercial CMV PCR kit. Infected infants underwent further evaluation at the hospital. RESULTS: CMV infection was identified in four of 1174 infants (0.34%) which is approximately 3 cases per 1000 live births. Infected infants were asymptomatic at birth and had a normal hearing status similar to other children. There were no factors in relation with CMV infection among newborns. CONCLUSION: According to the results of this study, infected infants with congenital CMV infection could identify at early stage by testing Guthrie cards (within 21 days of life). Furthermore, since there is a lack of CMV knowledge in our population, educating and effective counseling by obstetricians/ gynecologists to the pregnant women are recommended.
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spelling pubmed-73406122020-07-17 Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards Noorbakhsh, Samileh Farhadi, Mohammad Haghighi, Faezeh Minaeian, Sara Hasanabad, Morteza Haghighi Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Cytomegalovirus (CMV) constitutes the most common viral cause of congenital infections in newborns worldwide. There are a significant number of asymptomatic newborns with congenital CMV infection in Iran, which may develop long-term sequelae of infection. Unfortunately, limited data exsists from Iran on the rate of congenital CMV infection among neonates. The current study was aimed to investigate the prevalence of congenital CMV infection among Iranian neonates by testing Guthrie cards. MATERIALS AND METHODS: Guthrie cards were collected from infants within 2 weeks of life, and total DNA was extracted from samples by thermal shock and evaluated for CMV DNA using nested-PCR assay. CMV infection in newborns was confirmed through a commercial CMV PCR kit. Infected infants underwent further evaluation at the hospital. RESULTS: CMV infection was identified in four of 1174 infants (0.34%) which is approximately 3 cases per 1000 live births. Infected infants were asymptomatic at birth and had a normal hearing status similar to other children. There were no factors in relation with CMV infection among newborns. CONCLUSION: According to the results of this study, infected infants with congenital CMV infection could identify at early stage by testing Guthrie cards (within 21 days of life). Furthermore, since there is a lack of CMV knowledge in our population, educating and effective counseling by obstetricians/ gynecologists to the pregnant women are recommended. Tehran University of Medical Sciences 2020-06 /pmc/articles/PMC7340612/ /pubmed/32685115 Text en Copyright© 2020 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Noorbakhsh, Samileh
Farhadi, Mohammad
Haghighi, Faezeh
Minaeian, Sara
Hasanabad, Morteza Haghighi
Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards
title Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards
title_full Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards
title_fullStr Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards
title_full_unstemmed Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards
title_short Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards
title_sort neonatal screening for congenital cytomegalovirus infection in tehran, iran, using guthrie cards
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340612/
https://www.ncbi.nlm.nih.gov/pubmed/32685115
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