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Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study

BACKGROUND: The three-parent assisted reproductive technique may increase oocyte competence. OBJECTIVE: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asy...

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Autores principales: Darbandi, Sara, Darbandi, Mahsa, Agarwal, Ashok, khorshid, Hamid Reza Khorram, Sadeghi, Mohammad Reza, Esteves, Sandro C., Sengupta, Pallav, Dutta, Sulagna, Fathi, Zohreh, Zeraati, Hojjat, Mehdi Akhondi, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Knowledge E 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340984/
https://www.ncbi.nlm.nih.gov/pubmed/32754678
http://dx.doi.org/10.18502/ijrm.v13i6.7284
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author Darbandi, Sara
Darbandi, Mahsa
Agarwal, Ashok
khorshid, Hamid Reza Khorram
Sadeghi, Mohammad Reza
Esteves, Sandro C.
Sengupta, Pallav
Dutta, Sulagna
Fathi, Zohreh
Zeraati, Hojjat
Mehdi Akhondi, Mohammad
author_facet Darbandi, Sara
Darbandi, Mahsa
Agarwal, Ashok
khorshid, Hamid Reza Khorram
Sadeghi, Mohammad Reza
Esteves, Sandro C.
Sengupta, Pallav
Dutta, Sulagna
Fathi, Zohreh
Zeraati, Hojjat
Mehdi Akhondi, Mohammad
author_sort Darbandi, Sara
collection PubMed
description BACKGROUND: The three-parent assisted reproductive technique may increase oocyte competence. OBJECTIVE: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated. MATERIALS AND METHODS: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups. RESULTS: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001). CONCLUSION: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes.
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spelling pubmed-73409842020-08-03 Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study Darbandi, Sara Darbandi, Mahsa Agarwal, Ashok khorshid, Hamid Reza Khorram Sadeghi, Mohammad Reza Esteves, Sandro C. Sengupta, Pallav Dutta, Sulagna Fathi, Zohreh Zeraati, Hojjat Mehdi Akhondi, Mohammad Int J Reprod Biomed Research Article BACKGROUND: The three-parent assisted reproductive technique may increase oocyte competence. OBJECTIVE: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated. MATERIALS AND METHODS: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups. RESULTS: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001). CONCLUSION: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes. Knowledge E 2020-06-30 /pmc/articles/PMC7340984/ /pubmed/32754678 http://dx.doi.org/10.18502/ijrm.v13i6.7284 Text en Copyright © 2020 Darbandi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Article
Darbandi, Sara
Darbandi, Mahsa
Agarwal, Ashok
khorshid, Hamid Reza Khorram
Sadeghi, Mohammad Reza
Esteves, Sandro C.
Sengupta, Pallav
Dutta, Sulagna
Fathi, Zohreh
Zeraati, Hojjat
Mehdi Akhondi, Mohammad
Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
title Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
title_full Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
title_fullStr Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
title_full_unstemmed Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
title_short Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
title_sort comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: a case-control study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340984/
https://www.ncbi.nlm.nih.gov/pubmed/32754678
http://dx.doi.org/10.18502/ijrm.v13i6.7284
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