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Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
BACKGROUND: The three-parent assisted reproductive technique may increase oocyte competence. OBJECTIVE: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asy...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Knowledge E
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340984/ https://www.ncbi.nlm.nih.gov/pubmed/32754678 http://dx.doi.org/10.18502/ijrm.v13i6.7284 |
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author | Darbandi, Sara Darbandi, Mahsa Agarwal, Ashok khorshid, Hamid Reza Khorram Sadeghi, Mohammad Reza Esteves, Sandro C. Sengupta, Pallav Dutta, Sulagna Fathi, Zohreh Zeraati, Hojjat Mehdi Akhondi, Mohammad |
author_facet | Darbandi, Sara Darbandi, Mahsa Agarwal, Ashok khorshid, Hamid Reza Khorram Sadeghi, Mohammad Reza Esteves, Sandro C. Sengupta, Pallav Dutta, Sulagna Fathi, Zohreh Zeraati, Hojjat Mehdi Akhondi, Mohammad |
author_sort | Darbandi, Sara |
collection | PubMed |
description | BACKGROUND: The three-parent assisted reproductive technique may increase oocyte competence. OBJECTIVE: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated. MATERIALS AND METHODS: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups. RESULTS: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001). CONCLUSION: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes. |
format | Online Article Text |
id | pubmed-7340984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Knowledge E |
record_format | MEDLINE/PubMed |
spelling | pubmed-73409842020-08-03 Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study Darbandi, Sara Darbandi, Mahsa Agarwal, Ashok khorshid, Hamid Reza Khorram Sadeghi, Mohammad Reza Esteves, Sandro C. Sengupta, Pallav Dutta, Sulagna Fathi, Zohreh Zeraati, Hojjat Mehdi Akhondi, Mohammad Int J Reprod Biomed Research Article BACKGROUND: The three-parent assisted reproductive technique may increase oocyte competence. OBJECTIVE: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated. MATERIALS AND METHODS: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups. RESULTS: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001). CONCLUSION: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes. Knowledge E 2020-06-30 /pmc/articles/PMC7340984/ /pubmed/32754678 http://dx.doi.org/10.18502/ijrm.v13i6.7284 Text en Copyright © 2020 Darbandi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Article Darbandi, Sara Darbandi, Mahsa Agarwal, Ashok khorshid, Hamid Reza Khorram Sadeghi, Mohammad Reza Esteves, Sandro C. Sengupta, Pallav Dutta, Sulagna Fathi, Zohreh Zeraati, Hojjat Mehdi Akhondi, Mohammad Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study |
title | Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study |
title_full | Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study |
title_fullStr | Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study |
title_full_unstemmed | Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study |
title_short | Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study |
title_sort | comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: a case-control study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340984/ https://www.ncbi.nlm.nih.gov/pubmed/32754678 http://dx.doi.org/10.18502/ijrm.v13i6.7284 |
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