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Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping

Gene introgression from wild species has been shown to be a feasible approach for fiber quality improvement in Upland cotton. Previously, we developed an interspecific G. mustelinum × G. hirsutum advanced-backcross population and mapped over one hundred QTL for fiber quality traits. In the current s...

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Autores principales: Chen, Qi, Wang, Wei, Wang, Caixiang, Zhang, Mi, Yu, Jiwen, Zhang, Yifei, Yuan, Baotong, Ding, Yunyun, Jones, Don C., Paterson, Andrew H., Chee, Peng W., Wang, Baohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341125/
https://www.ncbi.nlm.nih.gov/pubmed/32393539
http://dx.doi.org/10.1534/g3.120.401125
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author Chen, Qi
Wang, Wei
Wang, Caixiang
Zhang, Mi
Yu, Jiwen
Zhang, Yifei
Yuan, Baotong
Ding, Yunyun
Jones, Don C.
Paterson, Andrew H.
Chee, Peng W.
Wang, Baohua
author_facet Chen, Qi
Wang, Wei
Wang, Caixiang
Zhang, Mi
Yu, Jiwen
Zhang, Yifei
Yuan, Baotong
Ding, Yunyun
Jones, Don C.
Paterson, Andrew H.
Chee, Peng W.
Wang, Baohua
author_sort Chen, Qi
collection PubMed
description Gene introgression from wild species has been shown to be a feasible approach for fiber quality improvement in Upland cotton. Previously, we developed an interspecific G. mustelinum × G. hirsutum advanced-backcross population and mapped over one hundred QTL for fiber quality traits. In the current study, a trait-based selective genotyping approach was utilized to prioritize a small subset of introgression lines with high phenotypic values for different fiber quality traits, to simultaneously validate multiple fiber quality QTL in a single experiment. A total of 75 QTL were detected by CIM and/or single-marker analysis, including 11 significant marker-trait associations (P < 0.001) and three putative associations (P < 0.005) also reported in earlier studies. The QTL that have been validated include three each for fiber length, micronaire, and elongation, and one each for fiber strength and uniformity. Collectively, about 10% of the QTL previously reported have been validated here, indicating that selective genotyping has the potential to validate multiple marker-trait associations for different traits, especially those with a moderate to large-effect detected simultaneously in one experimental population. The G. mustelinum alleles contributed to improved fiber quality for all validated loci. The results from this study will lay the foundation for further fine mapping, marker-assisted selection and map-based gene cloning.
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spelling pubmed-73411252020-07-21 Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping Chen, Qi Wang, Wei Wang, Caixiang Zhang, Mi Yu, Jiwen Zhang, Yifei Yuan, Baotong Ding, Yunyun Jones, Don C. Paterson, Andrew H. Chee, Peng W. Wang, Baohua G3 (Bethesda) Investigations Gene introgression from wild species has been shown to be a feasible approach for fiber quality improvement in Upland cotton. Previously, we developed an interspecific G. mustelinum × G. hirsutum advanced-backcross population and mapped over one hundred QTL for fiber quality traits. In the current study, a trait-based selective genotyping approach was utilized to prioritize a small subset of introgression lines with high phenotypic values for different fiber quality traits, to simultaneously validate multiple fiber quality QTL in a single experiment. A total of 75 QTL were detected by CIM and/or single-marker analysis, including 11 significant marker-trait associations (P < 0.001) and three putative associations (P < 0.005) also reported in earlier studies. The QTL that have been validated include three each for fiber length, micronaire, and elongation, and one each for fiber strength and uniformity. Collectively, about 10% of the QTL previously reported have been validated here, indicating that selective genotyping has the potential to validate multiple marker-trait associations for different traits, especially those with a moderate to large-effect detected simultaneously in one experimental population. The G. mustelinum alleles contributed to improved fiber quality for all validated loci. The results from this study will lay the foundation for further fine mapping, marker-assisted selection and map-based gene cloning. Genetics Society of America 2020-05-11 /pmc/articles/PMC7341125/ /pubmed/32393539 http://dx.doi.org/10.1534/g3.120.401125 Text en Copyright © 2020 Chen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Chen, Qi
Wang, Wei
Wang, Caixiang
Zhang, Mi
Yu, Jiwen
Zhang, Yifei
Yuan, Baotong
Ding, Yunyun
Jones, Don C.
Paterson, Andrew H.
Chee, Peng W.
Wang, Baohua
Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping
title Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping
title_full Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping
title_fullStr Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping
title_full_unstemmed Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping
title_short Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping
title_sort validation of qtls for fiber quality introgressed from gossypium mustelinum by selective genotyping
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341125/
https://www.ncbi.nlm.nih.gov/pubmed/32393539
http://dx.doi.org/10.1534/g3.120.401125
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