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Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail
SIMPLE SUMMARY: Quail is an economically important type of poultry, valued for its high meat quality and abundant egg nutrition. It is also an important laboratory research animal, widely used in developmental biology and toxicology tests. Since the gut microbiota plays a vital role in the host’s gr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341218/ https://www.ncbi.nlm.nih.gov/pubmed/32526858 http://dx.doi.org/10.3390/ani10061006 |
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author | Du, Xizhong Xiang, Yun Lou, Fangfang Tu, Pingguang Zhang, Xiaojun Hu, Xujin Lyu, Wentao Xiao, Yingping |
author_facet | Du, Xizhong Xiang, Yun Lou, Fangfang Tu, Pingguang Zhang, Xiaojun Hu, Xujin Lyu, Wentao Xiao, Yingping |
author_sort | Du, Xizhong |
collection | PubMed |
description | SIMPLE SUMMARY: Quail is an economically important type of poultry, valued for its high meat quality and abundant egg nutrition. It is also an important laboratory research animal, widely used in developmental biology and toxicology tests. Since the gut microbiota plays a vital role in the host’s growth and health, we investigated the microbiota inhabiting the duodenum, jejunum, ileum, cecum, and colorectum of quail in the present study, using 16S rRNA gene sequencing and qPCR. The concentrations of short-chain fatty acids (SCFAs) were evaluated using gas chromatography. We found that the microbiota in the cecum was different from other intestinal sections and the enriched inhabitants of SCFA-producing bacterial genera made cecum the core locations of SCFA production in quail. The results of this study will provide fundamental data for further quail microbiology and functional studies. ABSTRACT: Quail is raised throughout China for egg and meat production. To deeply understand the gastrointestinal microbial composition and metabolites of quail, the present study characterized the microbiota inhabiting five intestinal locations of eight-week-old quail using 16S rRNA gene sequencing and qPCR, and evaluated the concentrations of short-chain fatty acids (SCFAs) in each individual location using gas chromatography. The results showed that Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Deferribacteres were the five most abundant phyla in the intestinal tract of quail. Firmicutes was largely dominant (>95%) in the small intestine, whereas Bacteroidetes increased significantly in the cecum (19.19%) and colorectum (8.09%). At the genus level, Lactobacillus was predominant in almost all sections (>50%) except in the cecum (7.26%), where Megamonas, Faecalibacterium, and Bacteroides were dominant. qPCR data indicated that the population sizes of both the total bacteria and proportions of the Firmicutes, Bacteroidetes, and Bacteroides group increased going from the proximal toward the distal end of the intestine in quail. The SCFA-producing bacterial genera Bacteroides, Faecalibacterium, Alistipes, Blautia, Parabacteroides, and Clostridium were of higher richness in the cecum and colorectum, where, accordingly, more SCFAs were produced. These findings will be helpful for the future study of quail microbiology, as well as its relationship with productive performance and health. |
format | Online Article Text |
id | pubmed-7341218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73412182020-07-14 Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail Du, Xizhong Xiang, Yun Lou, Fangfang Tu, Pingguang Zhang, Xiaojun Hu, Xujin Lyu, Wentao Xiao, Yingping Animals (Basel) Article SIMPLE SUMMARY: Quail is an economically important type of poultry, valued for its high meat quality and abundant egg nutrition. It is also an important laboratory research animal, widely used in developmental biology and toxicology tests. Since the gut microbiota plays a vital role in the host’s growth and health, we investigated the microbiota inhabiting the duodenum, jejunum, ileum, cecum, and colorectum of quail in the present study, using 16S rRNA gene sequencing and qPCR. The concentrations of short-chain fatty acids (SCFAs) were evaluated using gas chromatography. We found that the microbiota in the cecum was different from other intestinal sections and the enriched inhabitants of SCFA-producing bacterial genera made cecum the core locations of SCFA production in quail. The results of this study will provide fundamental data for further quail microbiology and functional studies. ABSTRACT: Quail is raised throughout China for egg and meat production. To deeply understand the gastrointestinal microbial composition and metabolites of quail, the present study characterized the microbiota inhabiting five intestinal locations of eight-week-old quail using 16S rRNA gene sequencing and qPCR, and evaluated the concentrations of short-chain fatty acids (SCFAs) in each individual location using gas chromatography. The results showed that Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Deferribacteres were the five most abundant phyla in the intestinal tract of quail. Firmicutes was largely dominant (>95%) in the small intestine, whereas Bacteroidetes increased significantly in the cecum (19.19%) and colorectum (8.09%). At the genus level, Lactobacillus was predominant in almost all sections (>50%) except in the cecum (7.26%), where Megamonas, Faecalibacterium, and Bacteroides were dominant. qPCR data indicated that the population sizes of both the total bacteria and proportions of the Firmicutes, Bacteroidetes, and Bacteroides group increased going from the proximal toward the distal end of the intestine in quail. The SCFA-producing bacterial genera Bacteroides, Faecalibacterium, Alistipes, Blautia, Parabacteroides, and Clostridium were of higher richness in the cecum and colorectum, where, accordingly, more SCFAs were produced. These findings will be helpful for the future study of quail microbiology, as well as its relationship with productive performance and health. MDPI 2020-06-09 /pmc/articles/PMC7341218/ /pubmed/32526858 http://dx.doi.org/10.3390/ani10061006 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Du, Xizhong Xiang, Yun Lou, Fangfang Tu, Pingguang Zhang, Xiaojun Hu, Xujin Lyu, Wentao Xiao, Yingping Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail |
title | Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail |
title_full | Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail |
title_fullStr | Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail |
title_full_unstemmed | Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail |
title_short | Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail |
title_sort | microbial community and short-chain fatty acid mapping in the intestinal tract of quail |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341218/ https://www.ncbi.nlm.nih.gov/pubmed/32526858 http://dx.doi.org/10.3390/ani10061006 |
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