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Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model

SIMPLE SUMMARY: This study was conducted to demonstrate how a vitrified embryo transfer procedure incurs phenotypic and molecular changes throughout life. This study reports the first evidence describing that embryonic manipulation during a vitrified embryo transfer cycle induced molecular modificat...

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Autores principales: Garcia-Dominguez, Ximo, Marco-Jiménez, Francisco, Peñaranda, David S., Vicente, José Salvador
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341293/
https://www.ncbi.nlm.nih.gov/pubmed/32560425
http://dx.doi.org/10.3390/ani10061043
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author Garcia-Dominguez, Ximo
Marco-Jiménez, Francisco
Peñaranda, David S.
Vicente, José Salvador
author_facet Garcia-Dominguez, Ximo
Marco-Jiménez, Francisco
Peñaranda, David S.
Vicente, José Salvador
author_sort Garcia-Dominguez, Ximo
collection PubMed
description SIMPLE SUMMARY: This study was conducted to demonstrate how a vitrified embryo transfer procedure incurs phenotypic and molecular changes throughout life. This study reports the first evidence describing that embryonic manipulation during a vitrified embryo transfer cycle induced molecular modifications, concerning oxidative phosphorylation and dysregulations in zinc and lipid metabolism in liver tissue, which has been reported as responsible for postnatal variations of the phenotype. ABSTRACT: Nowadays, assisted reproductive technologies (ARTs) are considered valuable contributors to our past, but a future without their use is inconceivable. However, in recent years, several studies have evidenced a potential impact of ART on long-term development in mammal species. To date, the long-term follow-up data are still limited. So far, studies have mainly focused on in vitro fertilization or in vitro culture, with information from gametes/embryos cryopreservation field being practically missing. Herein, we report an approach to determine whether a vitrified embryo transfer procedure would have long-term consequences on the offspring. Using the rabbit as a model, we compared animals derived from vitrified-transferred embryos versus those naturally conceived, studying the growth performance, plus the weight throughout life, and the internal organs/tissues phenotype. The healthy status was assessed over the hematological and biochemical parameters in peripheral blood. Additionally, a comparative proteomic analysis was conducted in the liver tissue to investigate molecular cues related to vitrified embryo transfer in an adult tissue. After vitrified embryo transfer, birth weight was increased, and the growth performance was diminished in a sex-specific manner. In addition, vitrified-transferred animals showed significantly lower body, liver and heart weights in adulthood. Molecular analyses revealed that vitrified embryo transfer triggers reprogramming of the liver proteome. Functional analysis of the differentially expressed proteins showed changes in relation to oxidative phosphorylation and dysregulations in the zinc and lipid metabolism, which has been reported as possible causes of a disturbed growth pattern. Therefore, we conclude that vitrified embryo transfer is not a neutral procedure, and it incurs long-term effects in the offspring both at phenotypic and molecular levels. These results described a striking example of the developmental plasticity exhibited by the mammalian embryo.
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spelling pubmed-73412932020-07-14 Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model Garcia-Dominguez, Ximo Marco-Jiménez, Francisco Peñaranda, David S. Vicente, José Salvador Animals (Basel) Article SIMPLE SUMMARY: This study was conducted to demonstrate how a vitrified embryo transfer procedure incurs phenotypic and molecular changes throughout life. This study reports the first evidence describing that embryonic manipulation during a vitrified embryo transfer cycle induced molecular modifications, concerning oxidative phosphorylation and dysregulations in zinc and lipid metabolism in liver tissue, which has been reported as responsible for postnatal variations of the phenotype. ABSTRACT: Nowadays, assisted reproductive technologies (ARTs) are considered valuable contributors to our past, but a future without their use is inconceivable. However, in recent years, several studies have evidenced a potential impact of ART on long-term development in mammal species. To date, the long-term follow-up data are still limited. So far, studies have mainly focused on in vitro fertilization or in vitro culture, with information from gametes/embryos cryopreservation field being practically missing. Herein, we report an approach to determine whether a vitrified embryo transfer procedure would have long-term consequences on the offspring. Using the rabbit as a model, we compared animals derived from vitrified-transferred embryos versus those naturally conceived, studying the growth performance, plus the weight throughout life, and the internal organs/tissues phenotype. The healthy status was assessed over the hematological and biochemical parameters in peripheral blood. Additionally, a comparative proteomic analysis was conducted in the liver tissue to investigate molecular cues related to vitrified embryo transfer in an adult tissue. After vitrified embryo transfer, birth weight was increased, and the growth performance was diminished in a sex-specific manner. In addition, vitrified-transferred animals showed significantly lower body, liver and heart weights in adulthood. Molecular analyses revealed that vitrified embryo transfer triggers reprogramming of the liver proteome. Functional analysis of the differentially expressed proteins showed changes in relation to oxidative phosphorylation and dysregulations in the zinc and lipid metabolism, which has been reported as possible causes of a disturbed growth pattern. Therefore, we conclude that vitrified embryo transfer is not a neutral procedure, and it incurs long-term effects in the offspring both at phenotypic and molecular levels. These results described a striking example of the developmental plasticity exhibited by the mammalian embryo. MDPI 2020-06-17 /pmc/articles/PMC7341293/ /pubmed/32560425 http://dx.doi.org/10.3390/ani10061043 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garcia-Dominguez, Ximo
Marco-Jiménez, Francisco
Peñaranda, David S.
Vicente, José Salvador
Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model
title Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model
title_full Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model
title_fullStr Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model
title_full_unstemmed Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model
title_short Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model
title_sort long-term phenotypic and proteomic changes following vitrified embryo transfer in the rabbit model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341293/
https://www.ncbi.nlm.nih.gov/pubmed/32560425
http://dx.doi.org/10.3390/ani10061043
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