Pregnancy Loss (28–110 Days of Pregnancy) in Holstein Cows: A Retrospective Study

SIMPLE SUMMARY: High-yield dairy cow farms have implemented high technified management for the last few decades, aiming at optimizing productions with the best animal welfare canons. A key point to achieve this is the reproductive performance. Around 12% of cattle suffer pregnancy loss during the late embryonic/early foetal period (between 28 and 110 day of pregnancy). Thus, our objective was to study the pregnancy losses occurring in eight different Spanish high-yielding Holstein dairy herds, in locations with severe heat stress during the summer, to examine the link between pregnancy loss and different management factors. Some factors, previously confirmed as significant ones, such as the technician who performed artificial insemination (AI), fixed-time or after observed oestrus AI, the bull used, type of semen or season, did not affect pregnancy loss in our study. Moreover, older cows (compared to heifers), first artificial inseminations (compared to ≥2nd ones) and pregnancies after fixed-time-AI (compared to AI after observed oestrus and natural breedings) were definitively associated to higher pregnancy loss. Therefore, farmers and consultants should adapt their prevention strategies relating to pregnancy loss, particularly, to the parity of the cattle and to type and rank of AI. ABSTRACT: The objective of this retrospective study was to investigate the prevalence of pregnancy loss (PL; between 28–110 pregnancy days) and its relationship with factors: farm, year (2015–2018), season, artificial insemination (AI)-rank, parity, AI-type (fixed-time vs. oestrus-AI), previous PL, days in milk (DIM), fixed-time-AI protocol, AI-technician, bull, and semen-type (sexed vs. conventional). Data of 19,437 Holstein cattle AIs from eight Spanish farms were studied. Overall conception rate was 34.3% (6696/19,437) and PL 12.3% (822/6696). The PL was more likely to occur in primiparous (10.8%, odds ratio (OR) = 1.35; p = 0.04) and multiparous (15.3%; OR = 2.02, p < 0.01) than in heifers (PL = 6.9%, reference). Pregnancies achieved with AI after observed oestrus and natural breedings were associated with less PL than pregnancies after fixed-time-AI (12.7 vs. 11.9%; OR = 0.12, p = 0.01). First AIs related to higher PL than ≥2nd AIs (PL = 13.8% vs. 11.2; OR = 0.73, p < 0.01). The factors season, fixed-time-AI protocol, DIM, bull, AI-technician, or type of semen were not significantly associated with PL. Therefore, farmers and consultants should adapt their preventive strategies relating to PL, particularly, to the parity of the cattle.