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Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats

BACKGROUND: Ketogenic diet has been used as supportive therapy in a range of conditions including epilepsy, diabetes mellitus, and cancer. OBJECTIVE: This study aimed to investigate the effects of long-term consumption of ketogenic diet on blood gas, hematological profiles, organ functions, and supe...

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Autores principales: Arsyad, Aryadi, Idris, Irfan, Rasyid, Andi A., Usman, Rezky A., Faradillah, Kiki R., Latif, Wa Ode U., Lubis, Zidni I., Aminuddin, Aminuddin, Yustisia, Ika, Djabir, Yulia Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341377/
https://www.ncbi.nlm.nih.gov/pubmed/32685205
http://dx.doi.org/10.1155/2020/3642035
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author Arsyad, Aryadi
Idris, Irfan
Rasyid, Andi A.
Usman, Rezky A.
Faradillah, Kiki R.
Latif, Wa Ode U.
Lubis, Zidni I.
Aminuddin, Aminuddin
Yustisia, Ika
Djabir, Yulia Y.
author_facet Arsyad, Aryadi
Idris, Irfan
Rasyid, Andi A.
Usman, Rezky A.
Faradillah, Kiki R.
Latif, Wa Ode U.
Lubis, Zidni I.
Aminuddin, Aminuddin
Yustisia, Ika
Djabir, Yulia Y.
author_sort Arsyad, Aryadi
collection PubMed
description BACKGROUND: Ketogenic diet has been used as supportive therapy in a range of conditions including epilepsy, diabetes mellitus, and cancer. OBJECTIVE: This study aimed to investigate the effects of long-term consumption of ketogenic diet on blood gas, hematological profiles, organ functions, and superoxide dismutase level in a rat model. MATERIALS AND METHODS: Fifteen male Wistar rats were divided into control (n = 8) and ketogenic (n = 7) groups. Controls received standard diet contained 52.20% of carbohydrates, 7.00% fat, and 15.25% protein; meanwhile, the ketogenic group received a high-fat-low-carbohydrate diet which contained 5.66% of carbohydrate, 86.19% fat, and 8.15% protein. All rats were caged individually and received 30g of either standard or high-fat-low-carbohydrate pellets. The experiment was carried out for 60 days before the blood samples were taken and analyzed to obtain blood gas, cell counts, organ biomarkers, and plasma antioxidant superoxide dismutase (SOD) levels. RESULTS: The rats subjected to ketogenic diet experienced a marked decrease in body weight, blood sugar, and increased blood ketones (p < 0.05). The average blood pH was 7.36 ± 0.02 and base excess was −5.57 ± 2.39 mOsm/L, which were significantly lower than controls (p < 0.05). Hematological analysis showed significantly lower erythrocyte, hemoglobin, and hematocrit levels. No significant changes were found in alanine aminotransferase, aspartate aminotransferase, urea, and creatinine levels, indicating normal liver and kidney functions. Nevertheless, plasma SOD level significantly reduced with ketogenic diet. CONCLUSION: Long-term ketogenic diet induces metabolic acidosis, anemia, and reduced antioxidant enzyme level in rats following 60 days of consuming high-fat-low-carbohydrate diet.
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spelling pubmed-73413772020-07-16 Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats Arsyad, Aryadi Idris, Irfan Rasyid, Andi A. Usman, Rezky A. Faradillah, Kiki R. Latif, Wa Ode U. Lubis, Zidni I. Aminuddin, Aminuddin Yustisia, Ika Djabir, Yulia Y. J Nutr Metab Research Article BACKGROUND: Ketogenic diet has been used as supportive therapy in a range of conditions including epilepsy, diabetes mellitus, and cancer. OBJECTIVE: This study aimed to investigate the effects of long-term consumption of ketogenic diet on blood gas, hematological profiles, organ functions, and superoxide dismutase level in a rat model. MATERIALS AND METHODS: Fifteen male Wistar rats were divided into control (n = 8) and ketogenic (n = 7) groups. Controls received standard diet contained 52.20% of carbohydrates, 7.00% fat, and 15.25% protein; meanwhile, the ketogenic group received a high-fat-low-carbohydrate diet which contained 5.66% of carbohydrate, 86.19% fat, and 8.15% protein. All rats were caged individually and received 30g of either standard or high-fat-low-carbohydrate pellets. The experiment was carried out for 60 days before the blood samples were taken and analyzed to obtain blood gas, cell counts, organ biomarkers, and plasma antioxidant superoxide dismutase (SOD) levels. RESULTS: The rats subjected to ketogenic diet experienced a marked decrease in body weight, blood sugar, and increased blood ketones (p < 0.05). The average blood pH was 7.36 ± 0.02 and base excess was −5.57 ± 2.39 mOsm/L, which were significantly lower than controls (p < 0.05). Hematological analysis showed significantly lower erythrocyte, hemoglobin, and hematocrit levels. No significant changes were found in alanine aminotransferase, aspartate aminotransferase, urea, and creatinine levels, indicating normal liver and kidney functions. Nevertheless, plasma SOD level significantly reduced with ketogenic diet. CONCLUSION: Long-term ketogenic diet induces metabolic acidosis, anemia, and reduced antioxidant enzyme level in rats following 60 days of consuming high-fat-low-carbohydrate diet. Hindawi 2020-06-19 /pmc/articles/PMC7341377/ /pubmed/32685205 http://dx.doi.org/10.1155/2020/3642035 Text en Copyright © 2020 Aryadi Arsyad et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arsyad, Aryadi
Idris, Irfan
Rasyid, Andi A.
Usman, Rezky A.
Faradillah, Kiki R.
Latif, Wa Ode U.
Lubis, Zidni I.
Aminuddin, Aminuddin
Yustisia, Ika
Djabir, Yulia Y.
Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats
title Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats
title_full Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats
title_fullStr Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats
title_full_unstemmed Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats
title_short Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats
title_sort long-term ketogenic diet induces metabolic acidosis, anemia, and oxidative stress in healthy wistar rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341377/
https://www.ncbi.nlm.nih.gov/pubmed/32685205
http://dx.doi.org/10.1155/2020/3642035
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