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Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid

SIMPLE SUMMARY: The aim of this study was to investigate the simultaneous measurement of two different renal markers (iohexol and p-aminohippuric acid) in the plasma of different poultry species as the gold standard method. The two markers reflect three different renal processes: glomerular filtrati...

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Autores principales: Stroobant, Lenka, Croubels, Siska, Dhondt, Laura, Millecam, Joske, De Baere, Siegrid, Gasthuys, Elke, Morrens, Joachim, Antonissen, Gunther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341488/
https://www.ncbi.nlm.nih.gov/pubmed/32545683
http://dx.doi.org/10.3390/ani10061027
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author Stroobant, Lenka
Croubels, Siska
Dhondt, Laura
Millecam, Joske
De Baere, Siegrid
Gasthuys, Elke
Morrens, Joachim
Antonissen, Gunther
author_facet Stroobant, Lenka
Croubels, Siska
Dhondt, Laura
Millecam, Joske
De Baere, Siegrid
Gasthuys, Elke
Morrens, Joachim
Antonissen, Gunther
author_sort Stroobant, Lenka
collection PubMed
description SIMPLE SUMMARY: The aim of this study was to investigate the simultaneous measurement of two different renal markers (iohexol and p-aminohippuric acid) in the plasma of different poultry species as the gold standard method. The two markers reflect three different renal processes: glomerular filtration, effective renal plasma flow, and tubular secretion. The rate at which the kidneys filter blood is called the glomerular filtration rate. The effective renal plasma flow is the volume of plasma that reaches the kidney per time unit. Tubular secretion can be defined as active transport from the peritubular capillaries to the renal tubules. A moderate correlation was observed between tubular secretion and the glomerular filtration rate. A good correlation was demonstrated between the effective renal plasma flow and the glomerular filtration rate. This might be useful to model both renal processes. This approach could support the further development and validation of clinical renal biomarkers. These markers can be useful in the case of a chronic renal disease or renal failure, for which repeated evaluations of the renal function are required. ABSTRACT: The aim of the current study was to investigate the simultaneous measurement of plasma p-aminohippuric acid (PAH) clearance as a potential marker to assess effective renal plasma flow (eRPF) and tubular secretion (TS), and the plasma clearance of iohexol (IOH) as a marker of the glomerular filtration rate in poultry species. The PAH was administered intravenously (IV) to broiler chickens, layers, turkeys, Muscovy ducks, and pigeons. Each animal received successively a single bolus dose of 10 mg PAH/kg bodyweight (BW) and 100 mg PAH/kg BW to assess the eRPF and TS, respectively. Simultaneously with both PAH administrations, a single IV bolus of 64.7 mg/kg BW of IOH was administered. A high linear correlation (R(2) = 0.79) between eRPF, based on the clearance of the low dose of PAH, and BW was observed for the poultry species. The correlation between TS, based on the clearance of the high dose of PAH, and BW was moderate (R(2) = 0.50). Finally, a moderate correlation (R(2) = 0.68) was demonstrated between GFR and eRPF and between GFR and TS (R(2) = 0.56). This presented pharmacokinetic approach of the simultaneous administration of IOH and PAH enabled a simultaneous evaluation of eRPF/TS and GFR, respectively, in different poultry species.
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spelling pubmed-73414882020-07-14 Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid Stroobant, Lenka Croubels, Siska Dhondt, Laura Millecam, Joske De Baere, Siegrid Gasthuys, Elke Morrens, Joachim Antonissen, Gunther Animals (Basel) Article SIMPLE SUMMARY: The aim of this study was to investigate the simultaneous measurement of two different renal markers (iohexol and p-aminohippuric acid) in the plasma of different poultry species as the gold standard method. The two markers reflect three different renal processes: glomerular filtration, effective renal plasma flow, and tubular secretion. The rate at which the kidneys filter blood is called the glomerular filtration rate. The effective renal plasma flow is the volume of plasma that reaches the kidney per time unit. Tubular secretion can be defined as active transport from the peritubular capillaries to the renal tubules. A moderate correlation was observed between tubular secretion and the glomerular filtration rate. A good correlation was demonstrated between the effective renal plasma flow and the glomerular filtration rate. This might be useful to model both renal processes. This approach could support the further development and validation of clinical renal biomarkers. These markers can be useful in the case of a chronic renal disease or renal failure, for which repeated evaluations of the renal function are required. ABSTRACT: The aim of the current study was to investigate the simultaneous measurement of plasma p-aminohippuric acid (PAH) clearance as a potential marker to assess effective renal plasma flow (eRPF) and tubular secretion (TS), and the plasma clearance of iohexol (IOH) as a marker of the glomerular filtration rate in poultry species. The PAH was administered intravenously (IV) to broiler chickens, layers, turkeys, Muscovy ducks, and pigeons. Each animal received successively a single bolus dose of 10 mg PAH/kg bodyweight (BW) and 100 mg PAH/kg BW to assess the eRPF and TS, respectively. Simultaneously with both PAH administrations, a single IV bolus of 64.7 mg/kg BW of IOH was administered. A high linear correlation (R(2) = 0.79) between eRPF, based on the clearance of the low dose of PAH, and BW was observed for the poultry species. The correlation between TS, based on the clearance of the high dose of PAH, and BW was moderate (R(2) = 0.50). Finally, a moderate correlation (R(2) = 0.68) was demonstrated between GFR and eRPF and between GFR and TS (R(2) = 0.56). This presented pharmacokinetic approach of the simultaneous administration of IOH and PAH enabled a simultaneous evaluation of eRPF/TS and GFR, respectively, in different poultry species. MDPI 2020-06-12 /pmc/articles/PMC7341488/ /pubmed/32545683 http://dx.doi.org/10.3390/ani10061027 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stroobant, Lenka
Croubels, Siska
Dhondt, Laura
Millecam, Joske
De Baere, Siegrid
Gasthuys, Elke
Morrens, Joachim
Antonissen, Gunther
Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid
title Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid
title_full Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid
title_fullStr Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid
title_full_unstemmed Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid
title_short Simultaneous Measurement of Glomerular Filtration Rate, Effective Renal Plasma Flow and Tubular Secretion in Different Poultry Species by Single Intravenous Bolus of Iohexol and Para-Aminohippuric Acid
title_sort simultaneous measurement of glomerular filtration rate, effective renal plasma flow and tubular secretion in different poultry species by single intravenous bolus of iohexol and para-aminohippuric acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341488/
https://www.ncbi.nlm.nih.gov/pubmed/32545683
http://dx.doi.org/10.3390/ani10061027
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