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shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究

OBJECTIVE: To investigate the effect of silencing LSD1 gene by RNA interference on the proliferation, apoptosis on human lymphocytic leukemia Jurkat cell line and its mechanism. METHODS: The hairpin-like oligonucleotide sequences targeting LSD1 gene was transfected into Jurkat cells by lipofectamine...

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Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial office of Chinese Journal of Hematology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342296/
https://www.ncbi.nlm.nih.gov/pubmed/26876255
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2016.01.011
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collection PubMed
description OBJECTIVE: To investigate the effect of silencing LSD1 gene by RNA interference on the proliferation, apoptosis on human lymphocytic leukemia Jurkat cell line and its mechanism. METHODS: The hairpin-like oligonucleotide sequences targeting LSD1 gene was transfected into Jurkat cells by lipofectamine™ 2000. The LSD1 mRNA and protein were detected by RQ-PCR and Western blot. Cell growth was determined by MTT. Cell apoptosis was analyzed by flow cytometry. The expression of Bcl-2, Bax, procaspase-3, and histone H3K4me, H3K4me2, H3K4me3, Act-H3, H3K9me were detected by Western blot. RESULTS: LSD1 mRNA was markedly suppressed by the shRNA targeting LSD1. LSD1 shRNA suppressed the proliferation and induced cells apoptosis of Jurkat cells. The cell apoptotic rate was (41.34±3.58)%, (3.45±1.54)%, (1.76±0.52)% in LSD1 shRNA, Neg-shRNA and Blank respectively, the difference among them was statistically significant (P<0.05). LSD1 shRNA down-regulated the expressions of Bcl-2 and procaspase-3, and up-regulated the expression of Bax. The methylation of H3K4me1, me2 and acetylation of Act-H3 improved without change of the methylation of H3K4me3. CONCLUSION: Deplete of LSD1 gene maybe through modifying the methylation of histone H3K4 to promote the cell apoptosis and inhibit cell growth in Jurkat cell line.
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spelling pubmed-73422962020-07-16 shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To investigate the effect of silencing LSD1 gene by RNA interference on the proliferation, apoptosis on human lymphocytic leukemia Jurkat cell line and its mechanism. METHODS: The hairpin-like oligonucleotide sequences targeting LSD1 gene was transfected into Jurkat cells by lipofectamine™ 2000. The LSD1 mRNA and protein were detected by RQ-PCR and Western blot. Cell growth was determined by MTT. Cell apoptosis was analyzed by flow cytometry. The expression of Bcl-2, Bax, procaspase-3, and histone H3K4me, H3K4me2, H3K4me3, Act-H3, H3K9me were detected by Western blot. RESULTS: LSD1 mRNA was markedly suppressed by the shRNA targeting LSD1. LSD1 shRNA suppressed the proliferation and induced cells apoptosis of Jurkat cells. The cell apoptotic rate was (41.34±3.58)%, (3.45±1.54)%, (1.76±0.52)% in LSD1 shRNA, Neg-shRNA and Blank respectively, the difference among them was statistically significant (P<0.05). LSD1 shRNA down-regulated the expressions of Bcl-2 and procaspase-3, and up-regulated the expression of Bax. The methylation of H3K4me1, me2 and acetylation of Act-H3 improved without change of the methylation of H3K4me3. CONCLUSION: Deplete of LSD1 gene maybe through modifying the methylation of histone H3K4 to promote the cell apoptosis and inhibit cell growth in Jurkat cell line. Editorial office of Chinese Journal of Hematology 2016-01 /pmc/articles/PMC7342296/ /pubmed/26876255 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2016.01.011 Text en 2016年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal.
spellingShingle 论著
shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究
title shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究
title_full shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究
title_fullStr shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究
title_full_unstemmed shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究
title_short shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究
title_sort shrna沉默lsd1基因对jurkat细胞增殖的影响及机制研究
topic 论著
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342296/
https://www.ncbi.nlm.nih.gov/pubmed/26876255
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2016.01.011
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