外周血造血干细胞采集对166名健康供者外周血细胞计数的近期影响

OBJECTIVE: To observe the short-term effects of hemogram in donors after peripheral blood stem cell (PBSC) collection and donors' tolerance. METHODS: A total of 166 related allogeneic donors were selected from The First Affiliated Hospital of Medical School of Zhejiang University between January 2013 and December 2014, including 86 male and 80 female. All donors accepted granulocyte-colony-stimulating factor (G-CSF) 5–10 µg·kg(−1)·d(−1) until collection finished and were measured by blood cells count before and after PBSC collection. RESULTS: After PBSC collection, the hemoglobin level decreased from 145 (94–181) g/L to 138 (93–167) g/L, and the platelet counts decreased in all donors from 231 (105–490) ×10(9)/L to 95 (39–210) ×10(9)/L. The amount of hemoglobin contamination in collection products was weak correlated with the decreased hemoglobin in peripheral blood (r=0.297, P=0.017), and the platelet contamination was high correlated with that decreased in peripheral blood (r=0.719, P<0.001). The decline of hemoglobin level after twice PBSC collection was of no significant difference between four groups in different ages (P≥0.05). The decline of platelet counts was out of a significant difference (P>0.05). In addition, the decline of hemoglobin level after once and twice PBSC collection was of a significant difference between four groups in different body mass index (BMI) (P=0.003 and P<0.001), especially in thinner group with obvious decrease. But the decline of platelet counts was out of a significant difference (P>0.05). CONCLUSION: The hemoglobin level decreased mildly in healthy allogeneic hematopoietic stem cell donors after PBSC collection and it is better to adjust parameters every time to ensure their safety for thinner donors. However, it will increase the risk of platelet decline, which is unrelated with ages and BMI and can be tolerated.