KIR3DL1基因在92例造血干细胞供者中的表达

OBJECTIVE: To study KIR3DL1 expression level on NK cell surface of normal donors for hematopoietic stem cell transplantation (HSCT). METHODS: Ninety-two donors were performed by using of KIR genotyping, HLA high resolution genotyping and KIR3DL1 expression level using sequence-based testing (SBT), PCR-sequence specific primer (SSP) and flow cytometry methods. RESULTS: In 92 donors, the frequencies of KIR-A/A, Bx1, Bx2 for common genotypes were 46.74% (43/92), 18.48% (17/92) and 9.78% (9/92) respectively (P<0.001) ; KIR-A, B1, B2, B3 for common KIR haplo-type were 70.33% (128/182), 10.99% (20/182), 7.14% (13/182) and 4.39% (8/182) respectively (P<0.001) ; the frequencies of HLA-BW4/BW4, HLA-BW4/BW6, BW6/BW6 ligands were 13.79%, 67.81% and 18.39% respectively (P<0.001). KIR3DL1 middle expression level among haplo-type KIR-A/A and KIR-Bx, KIR-B/B were 18.77% (3.11%–49.24%), 13.14% (1.70%–63.32%) and 0.37% (0.20%–2.60%) respectively (P<0.05). KIR3DL1 expression level[18.77% (3.11%–49.24%) ] in haplo-type KIR-A/A was higher than haplo-type KIR-Bx at the same time did not express 2DL2 group [11.20% (3.50%–36.08%) ] (P=0.019). KIR3DL1 expression level in recognition group (HLA-BW4 positive group) [17.61% (1.40%–49.24%) ] was higher than KIR3DL1 unrecognized group (HLA-BW4 negative group) [10.60% (3.50%–18.56%) ] (P=0.006). CONCLUSION: The expression levels of KIR3DL1 in different KIR genotypes, haplotypes and HLA ligands were statistically significance.