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前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响

OBJECTIVE: To investigate the effects of angular pyranocoumarin (±)-4′-O-acetyl-3′-O-angeloyl-cis-khellactone (APC) extracted from peucedanum praeruptoruon on the proliferation and apoptosis of U266 cells, and to explore its related mechanism. METHODS: APC was extracted by petroleum ether technique,...

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Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial office of Chinese Journal of Hematology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342426/
https://www.ncbi.nlm.nih.gov/pubmed/26632467
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2015.11.010
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collection PubMed
description OBJECTIVE: To investigate the effects of angular pyranocoumarin (±)-4′-O-acetyl-3′-O-angeloyl-cis-khellactone (APC) extracted from peucedanum praeruptoruon on the proliferation and apoptosis of U266 cells, and to explore its related mechanism. METHODS: APC was extracted by petroleum ether technique, and its purity was tested by high performance liquid chromatography, and its chemical structure was identified by magnetic resonance spectroscopy. U266 cells were treated with APC in various concentrations (0, 10, 20, 30, 40 µg/ml) for different durations (24 and 48 h). The inhibitive effect of APC on cell growth was detected by CCK-8 method. After U266 cells were incubated with APC (0, 10, 20, 30, 40 µg/ml) for 24 h, the apoptosis of cells were observed by flow cytometry stained with Annexin Ⅴ/PI and Hochest33342; the expression levels of caspase-3, 8, ERK, p-ERK, AKT and p-AKT protein were assayed by Western blot; the expression of hTERT mRNA was measured by RT-PCR. RESULTS: The purity of APC identified by magnetic resonance imaging was 98.8%. The proliferation of U266 cells was inhibited, and the apoptosis was induced in a time-and/or dose-dependent manner after treatment with APC. APC could upregulate the caspase-8, 3 protein expression and downregulate the p-ERK, p-AKT protein expression along with the increase of APC dose. APC also could downregulate the hTERT mRNA expression. CONCLUSION: Angular pyranocoumarin APC could inhibit the proliferation and induce the apoptosis of U266 cells. The probable mechanism might be achieved by upregulating caspase-8, 3 protein expression and downregulating p-ERK, P-AKT protein and the hTERT mRNA expression.
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spelling pubmed-73424262020-07-16 前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To investigate the effects of angular pyranocoumarin (±)-4′-O-acetyl-3′-O-angeloyl-cis-khellactone (APC) extracted from peucedanum praeruptoruon on the proliferation and apoptosis of U266 cells, and to explore its related mechanism. METHODS: APC was extracted by petroleum ether technique, and its purity was tested by high performance liquid chromatography, and its chemical structure was identified by magnetic resonance spectroscopy. U266 cells were treated with APC in various concentrations (0, 10, 20, 30, 40 µg/ml) for different durations (24 and 48 h). The inhibitive effect of APC on cell growth was detected by CCK-8 method. After U266 cells were incubated with APC (0, 10, 20, 30, 40 µg/ml) for 24 h, the apoptosis of cells were observed by flow cytometry stained with Annexin Ⅴ/PI and Hochest33342; the expression levels of caspase-3, 8, ERK, p-ERK, AKT and p-AKT protein were assayed by Western blot; the expression of hTERT mRNA was measured by RT-PCR. RESULTS: The purity of APC identified by magnetic resonance imaging was 98.8%. The proliferation of U266 cells was inhibited, and the apoptosis was induced in a time-and/or dose-dependent manner after treatment with APC. APC could upregulate the caspase-8, 3 protein expression and downregulate the p-ERK, p-AKT protein expression along with the increase of APC dose. APC also could downregulate the hTERT mRNA expression. CONCLUSION: Angular pyranocoumarin APC could inhibit the proliferation and induce the apoptosis of U266 cells. The probable mechanism might be achieved by upregulating caspase-8, 3 protein expression and downregulating p-ERK, P-AKT protein and the hTERT mRNA expression. Editorial office of Chinese Journal of Hematology 2015-11 /pmc/articles/PMC7342426/ /pubmed/26632467 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2015.11.010 Text en 2015年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal.
spellingShingle 论著
前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响
title 前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响
title_full 前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响
title_fullStr 前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响
title_full_unstemmed 前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响
title_short 前胡提取物角型吡喃骈香豆素对U266细胞增殖和凋亡的影响
title_sort 前胡提取物角型吡喃骈香豆素对u266细胞增殖和凋亡的影响
topic 论著
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342426/
https://www.ncbi.nlm.nih.gov/pubmed/26632467
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2015.11.010
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