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Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义
OBJECTIVE: To investigate the expression characteristics of Tim-3 on natural killer (NK) cells of peripheral blood in patients with acute myeloid leukemia (AML) and its clinical significance. METHODS: Peripheral blood was obtained from 39 patients with newly diagnosed AML before intervention, with p...
Formato: | Online Artículo Texto |
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Lenguaje: | English |
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Editorial office of Chinese Journal of Hematology
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342451/ https://www.ncbi.nlm.nih.gov/pubmed/31648478 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.09.009 |
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collection | PubMed |
description | OBJECTIVE: To investigate the expression characteristics of Tim-3 on natural killer (NK) cells of peripheral blood in patients with acute myeloid leukemia (AML) and its clinical significance. METHODS: Peripheral blood was obtained from 39 patients with newly diagnosed AML before intervention, with peripheral blood from 28 cases of healthy volunteers collected as normal control. Using CD3, CD56 and Tim-3 as markers, expression levels of Tim-3 on the peripheral blood NK cells were detected by immune fluorescence labeling and flow cytometry. RESULTS: The ratio of the peripheral blood CD3(−)CD56(+) NK cells in newly diagnosed AML patients (5.74±5.31) %decreased significantly, compared with the normal control (12.55±6.33) % (t=4.596, P<0.001). Tim-3 expression on the peripheral blood NK cells in newly diagnosed AML patients (42.67±19.08) % decreased significantly, compared with the normal control group (60.99±20.69) % (t=3.781, P<0.001). CD3(−)CD56(+)NK cell ratio of peripheral blood in AML patients was significantly correlated with Chromosome karyotype (t=2.915,P<0.005). Expression level of Tim-3 on NK cells in the peripheral blood of AML patients had significant correlation with ratio of CR and NCCN high risk group (P<0.05). CONCLUSION: The rate of NK cells in peripheral blood and the expression level of Tim-3 on NK cells in AML patients decreased significantly.The lower expression level of Tim-3 on NK cells correlate with prognosis of AML. |
format | Online Article Text |
id | pubmed-7342451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Editorial office of Chinese Journal of Hematology |
record_format | MEDLINE/PubMed |
spelling | pubmed-73424512020-07-16 Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To investigate the expression characteristics of Tim-3 on natural killer (NK) cells of peripheral blood in patients with acute myeloid leukemia (AML) and its clinical significance. METHODS: Peripheral blood was obtained from 39 patients with newly diagnosed AML before intervention, with peripheral blood from 28 cases of healthy volunteers collected as normal control. Using CD3, CD56 and Tim-3 as markers, expression levels of Tim-3 on the peripheral blood NK cells were detected by immune fluorescence labeling and flow cytometry. RESULTS: The ratio of the peripheral blood CD3(−)CD56(+) NK cells in newly diagnosed AML patients (5.74±5.31) %decreased significantly, compared with the normal control (12.55±6.33) % (t=4.596, P<0.001). Tim-3 expression on the peripheral blood NK cells in newly diagnosed AML patients (42.67±19.08) % decreased significantly, compared with the normal control group (60.99±20.69) % (t=3.781, P<0.001). CD3(−)CD56(+)NK cell ratio of peripheral blood in AML patients was significantly correlated with Chromosome karyotype (t=2.915,P<0.005). Expression level of Tim-3 on NK cells in the peripheral blood of AML patients had significant correlation with ratio of CR and NCCN high risk group (P<0.05). CONCLUSION: The rate of NK cells in peripheral blood and the expression level of Tim-3 on NK cells in AML patients decreased significantly.The lower expression level of Tim-3 on NK cells correlate with prognosis of AML. Editorial office of Chinese Journal of Hematology 2019-09 /pmc/articles/PMC7342451/ /pubmed/31648478 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.09.009 Text en 2019年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal. |
spellingShingle | 论著 Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义 |
title | Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义 |
title_full | Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义 |
title_fullStr | Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义 |
title_full_unstemmed | Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义 |
title_short | Tim-3在急性髓系白血病患者NK细胞上的表达特征及其临床意义 |
title_sort | tim-3在急性髓系白血病患者nk细胞上的表达特征及其临床意义 |
topic | 论著 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342451/ https://www.ncbi.nlm.nih.gov/pubmed/31648478 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.09.009 |
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