Cargando…

Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer

INTRODUCTION: Immune therapy has shown good results in small-cell lung cancer (SCLC), but the impact of immune microenvironment of the disease is unclear. In this work, we detected expression of programmed death 1 (PD-1), PD-ligand 1 (PD-L1), and other immune biomarkers of cancer. We also analyzed t...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Chenglong, Zhang, Liping, Zhang, Wei, Liu, Yu, Chen, Bin, Zhao, Sha, Li, Wei, Wang, Lei, Ye, Lingyun, Jia, Keyi, Wang, Hao, Wu, Chunyan, He, Yayi, Zhou, Caicun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342461/
https://www.ncbi.nlm.nih.gov/pubmed/32753888
http://dx.doi.org/10.2147/OTT.S252031
_version_ 1783555494033489920
author Sun, Chenglong
Zhang, Liping
Zhang, Wei
Liu, Yu
Chen, Bin
Zhao, Sha
Li, Wei
Wang, Lei
Ye, Lingyun
Jia, Keyi
Wang, Hao
Wu, Chunyan
He, Yayi
Zhou, Caicun
author_facet Sun, Chenglong
Zhang, Liping
Zhang, Wei
Liu, Yu
Chen, Bin
Zhao, Sha
Li, Wei
Wang, Lei
Ye, Lingyun
Jia, Keyi
Wang, Hao
Wu, Chunyan
He, Yayi
Zhou, Caicun
author_sort Sun, Chenglong
collection PubMed
description INTRODUCTION: Immune therapy has shown good results in small-cell lung cancer (SCLC), but the impact of immune microenvironment of the disease is unclear. In this work, we detected expression of programmed death 1 (PD-1), PD-ligand 1 (PD-L1), and other immune biomarkers of cancer. We also analyzed the correlations between these markers and survival in SCLC. PATIENTS AND METHODS: Protein expression of PD-1, PD-L1, PD-L2, CD3, CD4, CD8, and FOXP3 was analyzed in surgical tissues from 102 SCLC patients by immunohistochemistry. RESULTS: Positive expression of PD-1 on tumor-infiltrating lymphocytes (TILs) was found in 40.2% of patients; 37.3% of patients showed positive expression of PD-L1 on TILs; and 3.9% showed positive expression of PD-L1 on tumor cells. PD-L2 protein was not expressed on tumor cells or TILs. Survival analysis showed that positive expression of PD-L1 on TILs was correlated with longer relapse-free survival (RFS) (p=0.004). Positive expression of PD-1 combined with a high ratio of lymphocytes (CD3, p=0.004; CD4, p=0.011; CD8, p=0.009; FOXP3, p=0.009) was associated with significantly better RFS than negative expression of PD-1 combined with a lower ratio of lymphocytes. Positive expression of PD-L1 combined with a high ratio of lymphocytes (CD3, p<0.001; CD4, p=0.001; CD8, p=0.002; FOXP3, p=0.001) was associated with significantly better RFS than negative expression of PD-L1 combined with a lower ratio of lymphocytes. All patients’ stage were between I and III. CONCLUSION: PD-1 and PD-L1 expression might be good prognostic factors in SCLC.
format Online
Article
Text
id pubmed-7342461
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-73424612020-08-03 Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer Sun, Chenglong Zhang, Liping Zhang, Wei Liu, Yu Chen, Bin Zhao, Sha Li, Wei Wang, Lei Ye, Lingyun Jia, Keyi Wang, Hao Wu, Chunyan He, Yayi Zhou, Caicun Onco Targets Ther Original Research INTRODUCTION: Immune therapy has shown good results in small-cell lung cancer (SCLC), but the impact of immune microenvironment of the disease is unclear. In this work, we detected expression of programmed death 1 (PD-1), PD-ligand 1 (PD-L1), and other immune biomarkers of cancer. We also analyzed the correlations between these markers and survival in SCLC. PATIENTS AND METHODS: Protein expression of PD-1, PD-L1, PD-L2, CD3, CD4, CD8, and FOXP3 was analyzed in surgical tissues from 102 SCLC patients by immunohistochemistry. RESULTS: Positive expression of PD-1 on tumor-infiltrating lymphocytes (TILs) was found in 40.2% of patients; 37.3% of patients showed positive expression of PD-L1 on TILs; and 3.9% showed positive expression of PD-L1 on tumor cells. PD-L2 protein was not expressed on tumor cells or TILs. Survival analysis showed that positive expression of PD-L1 on TILs was correlated with longer relapse-free survival (RFS) (p=0.004). Positive expression of PD-1 combined with a high ratio of lymphocytes (CD3, p=0.004; CD4, p=0.011; CD8, p=0.009; FOXP3, p=0.009) was associated with significantly better RFS than negative expression of PD-1 combined with a lower ratio of lymphocytes. Positive expression of PD-L1 combined with a high ratio of lymphocytes (CD3, p<0.001; CD4, p=0.001; CD8, p=0.002; FOXP3, p=0.001) was associated with significantly better RFS than negative expression of PD-L1 combined with a lower ratio of lymphocytes. All patients’ stage were between I and III. CONCLUSION: PD-1 and PD-L1 expression might be good prognostic factors in SCLC. Dove 2020-07-03 /pmc/articles/PMC7342461/ /pubmed/32753888 http://dx.doi.org/10.2147/OTT.S252031 Text en © 2020 Sun et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Sun, Chenglong
Zhang, Liping
Zhang, Wei
Liu, Yu
Chen, Bin
Zhao, Sha
Li, Wei
Wang, Lei
Ye, Lingyun
Jia, Keyi
Wang, Hao
Wu, Chunyan
He, Yayi
Zhou, Caicun
Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer
title Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer
title_full Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer
title_fullStr Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer
title_full_unstemmed Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer
title_short Expression of PD-1 and PD-L1 on Tumor-Infiltrating Lymphocytes Predicts Prognosis in Patients with Small-Cell Lung Cancer
title_sort expression of pd-1 and pd-l1 on tumor-infiltrating lymphocytes predicts prognosis in patients with small-cell lung cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342461/
https://www.ncbi.nlm.nih.gov/pubmed/32753888
http://dx.doi.org/10.2147/OTT.S252031
work_keys_str_mv AT sunchenglong expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT zhangliping expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT zhangwei expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT liuyu expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT chenbin expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT zhaosha expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT liwei expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT wanglei expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT yelingyun expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT jiakeyi expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT wanghao expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT wuchunyan expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT heyayi expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer
AT zhoucaicun expressionofpd1andpdl1ontumorinfiltratinglymphocytespredictsprognosisinpatientswithsmallcelllungcancer