地西他滨对急性T淋巴细胞白血病细胞株Molt4细胞的作用及其机制研究

OBJECTIVE: To explore the effects and possible mechanisms of decitabine on Molt4 in vitro. METHODS: Effects of decitabine on cells proliferation were detected by using CCK-8, the apoptosis by Annexin Ⅴ-FITC, cell cycles by propidium iodide-FACS. Discrepancy genes were screened by RNA-seq technique. The CpG methylation of lactoferrin (LTF) gene in Molt4 cells were identified by Bisulfite sequencing PCR (BSP). The expression of LTF mRNA in Molt4 by RT-PCR and LTF protein expression were analyzed by Western blot. RESULTS: Decitabine effectively inhibited proliferation and induced apoptosis for Molt4 cells by an time- and dose-dependent manners. Cell cycles were arrested at the G(0)/G(1) phase. The promoter methylation degree of LTF gene in Molt4 cells was 72.3% before decitabine treatment and decreased to 45.0% after treatment with 0.50 µmol/L decitabine for 72 h. After the reduction of methylation, expression of its mRNA and protein increased, meanwhile caspase 3 and caspase 9 protein expression levels increased. CONCLUSION: The demethylating drug decitabine can induce apoptosis, detain cell cycle at phase G(0)/G(1), inhibit proliferation and up-regulate LTF gene expression in Molt4 cells. LTF may become a new target for acute T lymphoblastic leukemia.