Sensitivity of yeast to lithium chloride connects the activity of YTA6 and YPR096C to translation of structured mRNAs

Lithium Chloride (LiCl) toxicity, mode of action and cellular responses have been the subject of active investigations over the past decades. In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar meta...

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Detalles Bibliográficos
Autores principales: Hajikarimlou, Maryam, Moteshareie, Houman, Omidi, Katayoun, Hooshyar, Mohsen, Shaikho, Sarah, Kazmirchuk, Tom, Burnside, Daniel, Takallou, Sarah, Zare, Narges, Jagadeesan, Sasi Kumar, Puchacz, Nathalie, Babu, Mohan, Smith, Myron, Holcik, Martin, Samanfar, Bahram, Golshani, Ashkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343135/
https://www.ncbi.nlm.nih.gov/pubmed/32639961
http://dx.doi.org/10.1371/journal.pone.0235033
Descripción
Sumario:Lithium Chloride (LiCl) toxicity, mode of action and cellular responses have been the subject of active investigations over the past decades. In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar metabolism. Reduced activity of phosphoglucomutase in the presence of galactose causes an accumulation of intermediate metabolites of galactose metabolism leading to a number of phenotypes including growth defect. In the current study, we identify two understudied yeast genes, YTA6 and YPR096C that when deleted, cell sensitivity to LiCl is increased when galactose is used as a carbon source. The 5’-UTR of PGM2 mRNA is structured. Using this region, we show that YTA6 and YPR096C influence the translation of PGM2 mRNA.

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