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Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles

Ovarian aging affects female reproductive potential and is characterized by alterations in proteins, mRNAs and non-coding RNAs inside the ovarian follicle. Ovarian somatic cells and the oocyte communicate with each other secreting different molecules into the follicular fluid, by extracellular vesic...

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Autores principales: Battaglia, Rosalia, Musumeci, Paolo, Ragusa, Marco, Barbagallo, Davide, Scalia, Marina, Zimbone, Massimo, Lo Faro, Josè Maria, Borzì, Placido, Scollo, Paolo, Purrello, Michele, Vento, Elena Maria, Di Pietro, Cinzia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343446/
https://www.ncbi.nlm.nih.gov/pubmed/32554857
http://dx.doi.org/10.18632/aging.103441
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author Battaglia, Rosalia
Musumeci, Paolo
Ragusa, Marco
Barbagallo, Davide
Scalia, Marina
Zimbone, Massimo
Lo Faro, Josè Maria
Borzì, Placido
Scollo, Paolo
Purrello, Michele
Vento, Elena Maria
Di Pietro, Cinzia
author_facet Battaglia, Rosalia
Musumeci, Paolo
Ragusa, Marco
Barbagallo, Davide
Scalia, Marina
Zimbone, Massimo
Lo Faro, Josè Maria
Borzì, Placido
Scollo, Paolo
Purrello, Michele
Vento, Elena Maria
Di Pietro, Cinzia
author_sort Battaglia, Rosalia
collection PubMed
description Ovarian aging affects female reproductive potential and is characterized by alterations in proteins, mRNAs and non-coding RNAs inside the ovarian follicle. Ovarian somatic cells and the oocyte communicate with each other secreting different molecules into the follicular fluid, by extracellular vesicles. The cargo of follicular fluid vesicles may influence female reproductive ability; accordingly, analysis of extracellular vesicle content could provide information about the quality of the female germ cell. In order to identify the most significant deregulated microRNAs in reproductive aging, we quantified the small extracellular vesicles in human follicular fluid from older and younger women and analyzed the expression of microRNAs enclosed inside the vesicles. We found twice as many small extracellular vesicles in the follicular fluid from older women and several differentially expressed microRNAs. Correlating microRNA expression profiles with vesicle number, we selected 46 deregulated microRNAs associated with aging. Bioinformatic analyses allowed us to identify six miRNAs involved in TP53 signaling pathways. Specifically, miR-16-5p, miR214-3p and miR-449a were downregulated and miR-125b, miR-155-5p and miR-372 were upregulated, influencing vesicle release, oocyte maturation and stress response. We believe that this approach allowed us to identify a battery of microRNAs strictly related to female reproductive aging.
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spelling pubmed-73434462020-07-15 Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles Battaglia, Rosalia Musumeci, Paolo Ragusa, Marco Barbagallo, Davide Scalia, Marina Zimbone, Massimo Lo Faro, Josè Maria Borzì, Placido Scollo, Paolo Purrello, Michele Vento, Elena Maria Di Pietro, Cinzia Aging (Albany NY) Research Paper Ovarian aging affects female reproductive potential and is characterized by alterations in proteins, mRNAs and non-coding RNAs inside the ovarian follicle. Ovarian somatic cells and the oocyte communicate with each other secreting different molecules into the follicular fluid, by extracellular vesicles. The cargo of follicular fluid vesicles may influence female reproductive ability; accordingly, analysis of extracellular vesicle content could provide information about the quality of the female germ cell. In order to identify the most significant deregulated microRNAs in reproductive aging, we quantified the small extracellular vesicles in human follicular fluid from older and younger women and analyzed the expression of microRNAs enclosed inside the vesicles. We found twice as many small extracellular vesicles in the follicular fluid from older women and several differentially expressed microRNAs. Correlating microRNA expression profiles with vesicle number, we selected 46 deregulated microRNAs associated with aging. Bioinformatic analyses allowed us to identify six miRNAs involved in TP53 signaling pathways. Specifically, miR-16-5p, miR214-3p and miR-449a were downregulated and miR-125b, miR-155-5p and miR-372 were upregulated, influencing vesicle release, oocyte maturation and stress response. We believe that this approach allowed us to identify a battery of microRNAs strictly related to female reproductive aging. Impact Journals 2020-06-17 /pmc/articles/PMC7343446/ /pubmed/32554857 http://dx.doi.org/10.18632/aging.103441 Text en Copyright © 2020 Battaglia et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Battaglia, Rosalia
Musumeci, Paolo
Ragusa, Marco
Barbagallo, Davide
Scalia, Marina
Zimbone, Massimo
Lo Faro, Josè Maria
Borzì, Placido
Scollo, Paolo
Purrello, Michele
Vento, Elena Maria
Di Pietro, Cinzia
Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles
title Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles
title_full Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles
title_fullStr Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles
title_full_unstemmed Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles
title_short Ovarian aging increases small extracellular vesicle CD81(+) release in human follicular fluid and influences miRNA profiles
title_sort ovarian aging increases small extracellular vesicle cd81(+) release in human follicular fluid and influences mirna profiles
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343446/
https://www.ncbi.nlm.nih.gov/pubmed/32554857
http://dx.doi.org/10.18632/aging.103441
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