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Factors affecting stability and infectivity of SARS-CoV-2

BACKGROUND: In late 2019, a novel human coronavirus – severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) – emerged in Wuhan, China. This virus has caused a global pandemic involving more than 200 countries. SARS-CoV-2 is highly adapted to humans and readily transmits from person-to-person....

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Autores principales: Chan, K-H., Sridhar, S., Zhang, R.R., Chu, H., Fung, A.Y-F., Chan, G., Chan, J.F-W., To, K.K-W., Hung, I.F-N., Cheng, V.C-C., Yuen, K-Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd on behalf of The Healthcare Infection Society. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343644/
https://www.ncbi.nlm.nih.gov/pubmed/32652214
http://dx.doi.org/10.1016/j.jhin.2020.07.009
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author Chan, K-H.
Sridhar, S.
Zhang, R.R.
Chu, H.
Fung, A.Y-F.
Chan, G.
Chan, J.F-W.
To, K.K-W.
Hung, I.F-N.
Cheng, V.C-C.
Yuen, K-Y.
author_facet Chan, K-H.
Sridhar, S.
Zhang, R.R.
Chu, H.
Fung, A.Y-F.
Chan, G.
Chan, J.F-W.
To, K.K-W.
Hung, I.F-N.
Cheng, V.C-C.
Yuen, K-Y.
author_sort Chan, K-H.
collection PubMed
description BACKGROUND: In late 2019, a novel human coronavirus – severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) – emerged in Wuhan, China. This virus has caused a global pandemic involving more than 200 countries. SARS-CoV-2 is highly adapted to humans and readily transmits from person-to-person. AIM: To investigate the infectivity of SARS-CoV-2 under various environmental and pH conditions. The efficacies of various laboratory virus inactivation methods and home disinfectants against SARS-CoV-2 were investigated. METHODS: The residual virus in dried form or in solution was titrated on to Vero E6 cells on days 0, 1, 3, 5 and 7 after incubation at different temperatures. Viral viability was determined after treatment with various disinfectants and pH solutions at room temperature (20–25(o)C). FINDINGS: SARS-CoV-2 was able to retain viability for 3–5 days in dried form or 7 days in solution at room temperature. SARS-CoV-2 could be detected under a wide range of pH conditions from pH 4 to pH 11 for several days, and for 1–2 days in stool at room temperature but lost 5 logs of infectivity. A variety of commonly used disinfectants and laboratory inactivation procedures were found to reduce viral viability effectively. CONCLUSION: This study demonstrated the stability of SARS-CoV-2 on environmental surfaces, and raises the possibility of faecal–oral transmission. Commonly used fixatives, nucleic acid extraction methods and heat inactivation were found to reduce viral infectivity significantly, which could ensure hospital and laboratory safety during the SARS-CoV-2 pandemic.
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spelling pubmed-73436442020-07-09 Factors affecting stability and infectivity of SARS-CoV-2 Chan, K-H. Sridhar, S. Zhang, R.R. Chu, H. Fung, A.Y-F. Chan, G. Chan, J.F-W. To, K.K-W. Hung, I.F-N. Cheng, V.C-C. Yuen, K-Y. J Hosp Infect Article BACKGROUND: In late 2019, a novel human coronavirus – severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) – emerged in Wuhan, China. This virus has caused a global pandemic involving more than 200 countries. SARS-CoV-2 is highly adapted to humans and readily transmits from person-to-person. AIM: To investigate the infectivity of SARS-CoV-2 under various environmental and pH conditions. The efficacies of various laboratory virus inactivation methods and home disinfectants against SARS-CoV-2 were investigated. METHODS: The residual virus in dried form or in solution was titrated on to Vero E6 cells on days 0, 1, 3, 5 and 7 after incubation at different temperatures. Viral viability was determined after treatment with various disinfectants and pH solutions at room temperature (20–25(o)C). FINDINGS: SARS-CoV-2 was able to retain viability for 3–5 days in dried form or 7 days in solution at room temperature. SARS-CoV-2 could be detected under a wide range of pH conditions from pH 4 to pH 11 for several days, and for 1–2 days in stool at room temperature but lost 5 logs of infectivity. A variety of commonly used disinfectants and laboratory inactivation procedures were found to reduce viral viability effectively. CONCLUSION: This study demonstrated the stability of SARS-CoV-2 on environmental surfaces, and raises the possibility of faecal–oral transmission. Commonly used fixatives, nucleic acid extraction methods and heat inactivation were found to reduce viral infectivity significantly, which could ensure hospital and laboratory safety during the SARS-CoV-2 pandemic. The Authors. Published by Elsevier Ltd on behalf of The Healthcare Infection Society. 2020-10 2020-07-09 /pmc/articles/PMC7343644/ /pubmed/32652214 http://dx.doi.org/10.1016/j.jhin.2020.07.009 Text en © 2020 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Chan, K-H.
Sridhar, S.
Zhang, R.R.
Chu, H.
Fung, A.Y-F.
Chan, G.
Chan, J.F-W.
To, K.K-W.
Hung, I.F-N.
Cheng, V.C-C.
Yuen, K-Y.
Factors affecting stability and infectivity of SARS-CoV-2
title Factors affecting stability and infectivity of SARS-CoV-2
title_full Factors affecting stability and infectivity of SARS-CoV-2
title_fullStr Factors affecting stability and infectivity of SARS-CoV-2
title_full_unstemmed Factors affecting stability and infectivity of SARS-CoV-2
title_short Factors affecting stability and infectivity of SARS-CoV-2
title_sort factors affecting stability and infectivity of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343644/
https://www.ncbi.nlm.nih.gov/pubmed/32652214
http://dx.doi.org/10.1016/j.jhin.2020.07.009
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