A marine photosynthetic microbial cell factory as a platform for spider silk production

Photosynthetic microorganisms such as cyanobacteria, purple bacteria and microalgae have attracted great interest as promising platforms for economical and sustainable production of bioenergy, biochemicals, and biopolymers. Here, we demonstrate heterotrophic production of spider dragline silk protei...

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Autores principales: Foong, Choon Pin, Higuchi-Takeuchi, Mieko, Malay, Ali D., Oktaviani, Nur Alia, Thagun, Chonprakun, Numata, Keiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343832/
https://www.ncbi.nlm.nih.gov/pubmed/32641733
http://dx.doi.org/10.1038/s42003-020-1099-6
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author Foong, Choon Pin
Higuchi-Takeuchi, Mieko
Malay, Ali D.
Oktaviani, Nur Alia
Thagun, Chonprakun
Numata, Keiji
author_facet Foong, Choon Pin
Higuchi-Takeuchi, Mieko
Malay, Ali D.
Oktaviani, Nur Alia
Thagun, Chonprakun
Numata, Keiji
author_sort Foong, Choon Pin
collection PubMed
description Photosynthetic microorganisms such as cyanobacteria, purple bacteria and microalgae have attracted great interest as promising platforms for economical and sustainable production of bioenergy, biochemicals, and biopolymers. Here, we demonstrate heterotrophic production of spider dragline silk proteins, major ampullate spidroins (MaSp), in a marine photosynthetic purple bacterium, Rhodovulum sulfidophilum, under both photoheterotrophic and photoautotrophic growth conditions. Spider silk is a biodegradable and biocompatible material with remarkable mechanical properties. R. sulfidophilum grow by utilizing abundant and renewable nonfood bioresources such as seawater, sunlight, and gaseous CO(2) and N(2), thus making this photosynthetic microbial cell factory a promising green and sustainable production platform for proteins and biopolymers, including spider silks.
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spelling pubmed-73438322020-07-13 A marine photosynthetic microbial cell factory as a platform for spider silk production Foong, Choon Pin Higuchi-Takeuchi, Mieko Malay, Ali D. Oktaviani, Nur Alia Thagun, Chonprakun Numata, Keiji Commun Biol Article Photosynthetic microorganisms such as cyanobacteria, purple bacteria and microalgae have attracted great interest as promising platforms for economical and sustainable production of bioenergy, biochemicals, and biopolymers. Here, we demonstrate heterotrophic production of spider dragline silk proteins, major ampullate spidroins (MaSp), in a marine photosynthetic purple bacterium, Rhodovulum sulfidophilum, under both photoheterotrophic and photoautotrophic growth conditions. Spider silk is a biodegradable and biocompatible material with remarkable mechanical properties. R. sulfidophilum grow by utilizing abundant and renewable nonfood bioresources such as seawater, sunlight, and gaseous CO(2) and N(2), thus making this photosynthetic microbial cell factory a promising green and sustainable production platform for proteins and biopolymers, including spider silks. Nature Publishing Group UK 2020-07-08 /pmc/articles/PMC7343832/ /pubmed/32641733 http://dx.doi.org/10.1038/s42003-020-1099-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Foong, Choon Pin
Higuchi-Takeuchi, Mieko
Malay, Ali D.
Oktaviani, Nur Alia
Thagun, Chonprakun
Numata, Keiji
A marine photosynthetic microbial cell factory as a platform for spider silk production
title A marine photosynthetic microbial cell factory as a platform for spider silk production
title_full A marine photosynthetic microbial cell factory as a platform for spider silk production
title_fullStr A marine photosynthetic microbial cell factory as a platform for spider silk production
title_full_unstemmed A marine photosynthetic microbial cell factory as a platform for spider silk production
title_short A marine photosynthetic microbial cell factory as a platform for spider silk production
title_sort marine photosynthetic microbial cell factory as a platform for spider silk production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343832/
https://www.ncbi.nlm.nih.gov/pubmed/32641733
http://dx.doi.org/10.1038/s42003-020-1099-6
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