Cargando…
A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells
Neuroregenerative medicine has witnessed impressive technological breakthroughs in recent years, but the currently available scaffold materials still have limitations regarding the development of effective treatment strategies for neurological diseases. Electrically conductive micropatterned materia...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344254/ https://www.ncbi.nlm.nih.gov/pubmed/32714901 http://dx.doi.org/10.3389/fbioe.2020.00616 |
_version_ | 1783555907115810816 |
---|---|
author | Yang, Yanru Zhang, Yuhua Chai, Renjie Gu, Zhongze |
author_facet | Yang, Yanru Zhang, Yuhua Chai, Renjie Gu, Zhongze |
author_sort | Yang, Yanru |
collection | PubMed |
description | Neuroregenerative medicine has witnessed impressive technological breakthroughs in recent years, but the currently available scaffold materials still have limitations regarding the development of effective treatment strategies for neurological diseases. Electrically conductive micropatterned materials have gained popularity in recent years due to their significant effects on neural stem cell fate. Polydopamine (PDA)—modified materials can also enhance the differentiation of neurons. In this work, we show that PDA-modified carbon microfiber skeleton composites have the appropriate conductivity, three-dimensional structure, and microenvironment regulation that are crucial for the growth of neural stem cells. The design we present is low-cost and easy to make and shows great promise for studying the growth and development of mouse neural stem cells. Our results show that the PDA-mediated formation of electrically conductive and viscous nanofiber webs promoted the adhesion, organization, and intercellular coupling of neural stem cells relative to the control group. PDA induced massive proliferation of neural stem cells and promoted the expression of Ki-67. Together, our results suggest that the composite material can be used as a multifunctional neural scaffold for clinical treatment and in vitro research by improving the structure, conductivity, and mechanical integrity of the regenerated tissues. |
format | Online Article Text |
id | pubmed-7344254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73442542020-07-25 A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells Yang, Yanru Zhang, Yuhua Chai, Renjie Gu, Zhongze Front Bioeng Biotechnol Bioengineering and Biotechnology Neuroregenerative medicine has witnessed impressive technological breakthroughs in recent years, but the currently available scaffold materials still have limitations regarding the development of effective treatment strategies for neurological diseases. Electrically conductive micropatterned materials have gained popularity in recent years due to their significant effects on neural stem cell fate. Polydopamine (PDA)—modified materials can also enhance the differentiation of neurons. In this work, we show that PDA-modified carbon microfiber skeleton composites have the appropriate conductivity, three-dimensional structure, and microenvironment regulation that are crucial for the growth of neural stem cells. The design we present is low-cost and easy to make and shows great promise for studying the growth and development of mouse neural stem cells. Our results show that the PDA-mediated formation of electrically conductive and viscous nanofiber webs promoted the adhesion, organization, and intercellular coupling of neural stem cells relative to the control group. PDA induced massive proliferation of neural stem cells and promoted the expression of Ki-67. Together, our results suggest that the composite material can be used as a multifunctional neural scaffold for clinical treatment and in vitro research by improving the structure, conductivity, and mechanical integrity of the regenerated tissues. Frontiers Media S.A. 2020-06-23 /pmc/articles/PMC7344254/ /pubmed/32714901 http://dx.doi.org/10.3389/fbioe.2020.00616 Text en Copyright © 2020 Yang, Zhang, Chai and Gu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Yang, Yanru Zhang, Yuhua Chai, Renjie Gu, Zhongze A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells |
title | A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells |
title_full | A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells |
title_fullStr | A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells |
title_full_unstemmed | A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells |
title_short | A Polydopamine-Functionalized Carbon Microfibrous Scaffold Accelerates the Development of Neural Stem Cells |
title_sort | polydopamine-functionalized carbon microfibrous scaffold accelerates the development of neural stem cells |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344254/ https://www.ncbi.nlm.nih.gov/pubmed/32714901 http://dx.doi.org/10.3389/fbioe.2020.00616 |
work_keys_str_mv | AT yangyanru apolydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells AT zhangyuhua apolydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells AT chairenjie apolydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells AT guzhongze apolydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells AT yangyanru polydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells AT zhangyuhua polydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells AT chairenjie polydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells AT guzhongze polydopaminefunctionalizedcarbonmicrofibrousscaffoldacceleratesthedevelopmentofneuralstemcells |