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Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish

This piece of research evaluates the presence of protease inhibitors in the macroalga Ulva ohnoi and provides an initial overview of their mode of action. The ability of Ulva protease inhibitors to inhibit digestive proteases of three marine fish species, as well as their capacity to hamper the hydr...

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Autores principales: Vizcaíno, Antonio Jesús, Galafat, Alba, Sáez, María Isabel, Martínez, Tomás Francisco, Alarcón, Francisco Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344585/
https://www.ncbi.nlm.nih.gov/pubmed/32570719
http://dx.doi.org/10.3390/md18060319
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author Vizcaíno, Antonio Jesús
Galafat, Alba
Sáez, María Isabel
Martínez, Tomás Francisco
Alarcón, Francisco Javier
author_facet Vizcaíno, Antonio Jesús
Galafat, Alba
Sáez, María Isabel
Martínez, Tomás Francisco
Alarcón, Francisco Javier
author_sort Vizcaíno, Antonio Jesús
collection PubMed
description This piece of research evaluates the presence of protease inhibitors in the macroalga Ulva ohnoi and provides an initial overview of their mode of action. The ability of Ulva protease inhibitors to inhibit digestive proteases of three marine fish species, as well as their capacity to hamper the hydrolysis of a reference protein by those fish proteases, were assessed. In addition, thermal stability and the mode of inhibition on trypsin and chymotrypsin were also studied. Dose-response inhibition curves and in vitro protein hydrolysis assays revealed a noticeable inhibition of fish enzymes when Ulva concentration increased in the assay. The thermal treatment of Ulva reduced markedly the inhibitory effect on fish digestive protease. Finally, Lineweaver–Burk plots indicated that trypsin and chymotrypsin inhibition consisted of a mixed-type inhibition mechanism in which the inhibitory effect depends on Ulva concentration. Overall, the results confirmed the presence of protease inhibitors in Ulva, though heat treatment was enough for inactivating these compounds.
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spelling pubmed-73445852020-07-09 Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish Vizcaíno, Antonio Jesús Galafat, Alba Sáez, María Isabel Martínez, Tomás Francisco Alarcón, Francisco Javier Mar Drugs Article This piece of research evaluates the presence of protease inhibitors in the macroalga Ulva ohnoi and provides an initial overview of their mode of action. The ability of Ulva protease inhibitors to inhibit digestive proteases of three marine fish species, as well as their capacity to hamper the hydrolysis of a reference protein by those fish proteases, were assessed. In addition, thermal stability and the mode of inhibition on trypsin and chymotrypsin were also studied. Dose-response inhibition curves and in vitro protein hydrolysis assays revealed a noticeable inhibition of fish enzymes when Ulva concentration increased in the assay. The thermal treatment of Ulva reduced markedly the inhibitory effect on fish digestive protease. Finally, Lineweaver–Burk plots indicated that trypsin and chymotrypsin inhibition consisted of a mixed-type inhibition mechanism in which the inhibitory effect depends on Ulva concentration. Overall, the results confirmed the presence of protease inhibitors in Ulva, though heat treatment was enough for inactivating these compounds. MDPI 2020-06-18 /pmc/articles/PMC7344585/ /pubmed/32570719 http://dx.doi.org/10.3390/md18060319 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vizcaíno, Antonio Jesús
Galafat, Alba
Sáez, María Isabel
Martínez, Tomás Francisco
Alarcón, Francisco Javier
Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish
title Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish
title_full Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish
title_fullStr Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish
title_full_unstemmed Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish
title_short Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish
title_sort partial characterization of protease inhibitors of ulva ohnoi and their effect on digestive proteases of marine fish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344585/
https://www.ncbi.nlm.nih.gov/pubmed/32570719
http://dx.doi.org/10.3390/md18060319
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