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Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study
Recent advances in phytomedicine have explored some potential candidates for nerve regeneration, including hydroxytyrosol (HT). This study was undertaken to explore the potential effects of HT on human Schwann cells’ proliferation. Methods: The primary human Schwann cell (hSC) was characterized, and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344605/ https://www.ncbi.nlm.nih.gov/pubmed/32575426 http://dx.doi.org/10.3390/ijerph17124404 |
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author | Kamil, Khidhir Yazid, Muhammad Dain Idrus, Ruszymah Bt Hj Kumar, Jaya |
author_facet | Kamil, Khidhir Yazid, Muhammad Dain Idrus, Ruszymah Bt Hj Kumar, Jaya |
author_sort | Kamil, Khidhir |
collection | PubMed |
description | Recent advances in phytomedicine have explored some potential candidates for nerve regeneration, including hydroxytyrosol (HT). This study was undertaken to explore the potential effects of HT on human Schwann cells’ proliferation. Methods: The primary human Schwann cell (hSC) was characterized, and the proliferation rate of hSC supplemented with various concentrations of HT was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis and protein expression of glial fibrillary acidic protein (GFAP) and p75 nerve growth factor receptor (p75 NGFR) were evaluated via the immunofluorescence technique. Results: In vitro culture of hSCs revealed spindle-like, bipolar morphology with the expression of specific markers of hSC. Hydroxytyrosol at 10 and 20 ng/mL significantly increased the proliferation of hSCs by 30.12 ± 5.9% and 47.8 ± 6.7% compared to control (p < 0.05). Cell cycle analysis showed that HT-treated hSCs have a higher proliferation index (16.2 ± 0.2%) than the control (12.4 ± 0.4%) (p < 0.01). In addition, HT significantly increased the protein expression of GFAP and p75NGFR (p < 0.05). Conclusion: HT stimulates the proliferation of hSCs in vitro, indicated by a significant increase in the hSC proliferation index and protein expression of hSCs’ proliferation markers, namely p75 NGFR and GFAP. |
format | Online Article Text |
id | pubmed-7344605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73446052020-07-09 Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study Kamil, Khidhir Yazid, Muhammad Dain Idrus, Ruszymah Bt Hj Kumar, Jaya Int J Environ Res Public Health Article Recent advances in phytomedicine have explored some potential candidates for nerve regeneration, including hydroxytyrosol (HT). This study was undertaken to explore the potential effects of HT on human Schwann cells’ proliferation. Methods: The primary human Schwann cell (hSC) was characterized, and the proliferation rate of hSC supplemented with various concentrations of HT was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis and protein expression of glial fibrillary acidic protein (GFAP) and p75 nerve growth factor receptor (p75 NGFR) were evaluated via the immunofluorescence technique. Results: In vitro culture of hSCs revealed spindle-like, bipolar morphology with the expression of specific markers of hSC. Hydroxytyrosol at 10 and 20 ng/mL significantly increased the proliferation of hSCs by 30.12 ± 5.9% and 47.8 ± 6.7% compared to control (p < 0.05). Cell cycle analysis showed that HT-treated hSCs have a higher proliferation index (16.2 ± 0.2%) than the control (12.4 ± 0.4%) (p < 0.01). In addition, HT significantly increased the protein expression of GFAP and p75NGFR (p < 0.05). Conclusion: HT stimulates the proliferation of hSCs in vitro, indicated by a significant increase in the hSC proliferation index and protein expression of hSCs’ proliferation markers, namely p75 NGFR and GFAP. MDPI 2020-06-19 2020-06 /pmc/articles/PMC7344605/ /pubmed/32575426 http://dx.doi.org/10.3390/ijerph17124404 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kamil, Khidhir Yazid, Muhammad Dain Idrus, Ruszymah Bt Hj Kumar, Jaya Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study |
title | Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study |
title_full | Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study |
title_fullStr | Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study |
title_full_unstemmed | Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study |
title_short | Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study |
title_sort | hydroxytyrosol promotes proliferation of human schwann cells: an in vitro study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344605/ https://www.ncbi.nlm.nih.gov/pubmed/32575426 http://dx.doi.org/10.3390/ijerph17124404 |
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