Lethal Outcome of Leptospirosis in Southern Russia: Characterization of Leptospira Interrogans Isolated from a Deсeased Teenager

This article describes a lethal case of leptospirosis that occurred in Southern Russia. The Leptospira strain was isolated and characterized using a microscopic agglutination test, MALDI-TOF mass spectrometry, targeted PCR, and high-throughput sequencing. We show that molecular and mass-spectrometry...

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Detalles Bibliográficos
Autores principales: Samoilov, Andrei E., Stoyanova, Nataliya A., Tokarevich, Nikolai K., Evengard, Birgitta, Zueva, Elena V., Panferova, Yulia A., Ostankova, Yulia V., Zueva, Elena B., Valutite, Diana E., Kovalev, Evgeniy V., Litovko, Anna R., Goncharov, Alexey U., Semenov, Alexandr V., Khafizov, Kamil, Dedkov, Vladimir G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344687/
https://www.ncbi.nlm.nih.gov/pubmed/32545855
http://dx.doi.org/10.3390/ijerph17124238
Descripción
Sumario:This article describes a lethal case of leptospirosis that occurred in Southern Russia. The Leptospira strain was isolated and characterized using a microscopic agglutination test, MALDI-TOF mass spectrometry, targeted PCR, and high-throughput sequencing. We show that molecular and mass-spectrometry methods can be an alternative to conventional methods of leptospirosis diagnostics and Leptospira study, which require highly qualified staff and can be performed only at specialized laboratories. We also report the first whole genome of L. interrogans isolated in Russia.

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