Cargando…

OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip

Improved in vitro models of human organs for predicting drug efficacy, interactions, and disease modelling are crucially needed to minimize the use of animal models, which inevitably display significant differences from the human disease state and metabolism. Inside the body, cells are organized eit...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramadan, Qasem, Gourikutty, Sajay Bhuvanendran Nair, Zhang, Qingxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344814/
https://www.ncbi.nlm.nih.gov/pubmed/32486495
http://dx.doi.org/10.3390/mi11060565
_version_ 1783556031343755264
author Ramadan, Qasem
Gourikutty, Sajay Bhuvanendran Nair
Zhang, Qingxin
author_facet Ramadan, Qasem
Gourikutty, Sajay Bhuvanendran Nair
Zhang, Qingxin
author_sort Ramadan, Qasem
collection PubMed
description Improved in vitro models of human organs for predicting drug efficacy, interactions, and disease modelling are crucially needed to minimize the use of animal models, which inevitably display significant differences from the human disease state and metabolism. Inside the body, cells are organized either in direct contact or in close proximity to other cell types in a tightly controlled architecture that regulates tissue function. To emulate this cellular interface in vitro, an advanced cell culture system is required. In this paper, we describe a set of compartmentalized silicon-based microfluidic chips that enable co-culturing several types of cells in close proximity with enhanced cell–cell interaction. In vivo-like fluid flow into and/or from each compartment, as well as between adjacent compartments, is maintained by micro-engineered porous barriers. This porous structure provides a tool for mimicking the paracrine exchange between cells in the human body. As a demonstrating example, the microfluidic system was tested by culturing human adipose tissue that is infiltrated with immune cells to study the role if the interplay between the two cells in the context of type 2 diabetes. However, the system provides a platform technology for mimicking the structure and function of single- and multi-organ models, which could significantly narrow the gap between in vivo and in vitro conditions.
format Online
Article
Text
id pubmed-7344814
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73448142020-07-09 OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip Ramadan, Qasem Gourikutty, Sajay Bhuvanendran Nair Zhang, Qingxin Micromachines (Basel) Article Improved in vitro models of human organs for predicting drug efficacy, interactions, and disease modelling are crucially needed to minimize the use of animal models, which inevitably display significant differences from the human disease state and metabolism. Inside the body, cells are organized either in direct contact or in close proximity to other cell types in a tightly controlled architecture that regulates tissue function. To emulate this cellular interface in vitro, an advanced cell culture system is required. In this paper, we describe a set of compartmentalized silicon-based microfluidic chips that enable co-culturing several types of cells in close proximity with enhanced cell–cell interaction. In vivo-like fluid flow into and/or from each compartment, as well as between adjacent compartments, is maintained by micro-engineered porous barriers. This porous structure provides a tool for mimicking the paracrine exchange between cells in the human body. As a demonstrating example, the microfluidic system was tested by culturing human adipose tissue that is infiltrated with immune cells to study the role if the interplay between the two cells in the context of type 2 diabetes. However, the system provides a platform technology for mimicking the structure and function of single- and multi-organ models, which could significantly narrow the gap between in vivo and in vitro conditions. MDPI 2020-05-31 /pmc/articles/PMC7344814/ /pubmed/32486495 http://dx.doi.org/10.3390/mi11060565 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramadan, Qasem
Gourikutty, Sajay Bhuvanendran Nair
Zhang, Qingxin
OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip
title OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip
title_full OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip
title_fullStr OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip
title_full_unstemmed OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip
title_short OOCHIP: Compartmentalized Microfluidic Perfusion System with Porous Barriers for Enhanced Cell–Cell Crosstalk in Organ-on-a-Chip
title_sort oochip: compartmentalized microfluidic perfusion system with porous barriers for enhanced cell–cell crosstalk in organ-on-a-chip
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344814/
https://www.ncbi.nlm.nih.gov/pubmed/32486495
http://dx.doi.org/10.3390/mi11060565
work_keys_str_mv AT ramadanqasem oochipcompartmentalizedmicrofluidicperfusionsystemwithporousbarriersforenhancedcellcellcrosstalkinorganonachip
AT gourikuttysajaybhuvanendrannair oochipcompartmentalizedmicrofluidicperfusionsystemwithporousbarriersforenhancedcellcellcrosstalkinorganonachip
AT zhangqingxin oochipcompartmentalizedmicrofluidicperfusionsystemwithporousbarriersforenhancedcellcellcrosstalkinorganonachip