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Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles
The sizes of most prokaryotic cells are several microns. It is very difficult to separate cells with similar sizes. A sorter with a contraction–expansion microchannel and applied magnetic field is designed to sort microparticles with diameters of 3, 4 and 5 microns. To evaluate the sorting efficienc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344843/ https://www.ncbi.nlm.nih.gov/pubmed/32486500 http://dx.doi.org/10.3390/mi11060566 |
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author | Du, Jiayou Li, Long Zhuo, Qiuyi Wang, Ruijin Zhu, Zefei |
author_facet | Du, Jiayou Li, Long Zhuo, Qiuyi Wang, Ruijin Zhu, Zefei |
author_sort | Du, Jiayou |
collection | PubMed |
description | The sizes of most prokaryotic cells are several microns. It is very difficult to separate cells with similar sizes. A sorter with a contraction–expansion microchannel and applied magnetic field is designed to sort microparticles with diameters of 3, 4 and 5 microns. To evaluate the sorting efficiency of the designed sorter, numerical simulations for calculating the distributions of microparticles with similar sizes were carried out for various magnetic fields, inlet velocities, sheath flow ratios and structural parameters. The numerical results indicate that micro-particles with diameters of 3, 4 and 5 microns can be sorted efficiently in such a sorter within appropriate parameters. Furthermore, it is shown that a bigger particle size and more powerful magnetic field can result in a greater lateral migration of microparticles. The sorting efficiency of microparticles promotes a lower inlet velocity and greater sheath flow ratios. A smaller contraction–expansion ratio can induce a greater space between particle-bands. Finally, the micro particle image velocity (micro-PIV) experiments were conducted to obtain the bandwidths and spaces between particle-bands. The comparisons between the numerical and experimental results show a good agreement and make the validity of the numerical results certain. |
format | Online Article Text |
id | pubmed-7344843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73448432020-07-09 Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles Du, Jiayou Li, Long Zhuo, Qiuyi Wang, Ruijin Zhu, Zefei Micromachines (Basel) Article The sizes of most prokaryotic cells are several microns. It is very difficult to separate cells with similar sizes. A sorter with a contraction–expansion microchannel and applied magnetic field is designed to sort microparticles with diameters of 3, 4 and 5 microns. To evaluate the sorting efficiency of the designed sorter, numerical simulations for calculating the distributions of microparticles with similar sizes were carried out for various magnetic fields, inlet velocities, sheath flow ratios and structural parameters. The numerical results indicate that micro-particles with diameters of 3, 4 and 5 microns can be sorted efficiently in such a sorter within appropriate parameters. Furthermore, it is shown that a bigger particle size and more powerful magnetic field can result in a greater lateral migration of microparticles. The sorting efficiency of microparticles promotes a lower inlet velocity and greater sheath flow ratios. A smaller contraction–expansion ratio can induce a greater space between particle-bands. Finally, the micro particle image velocity (micro-PIV) experiments were conducted to obtain the bandwidths and spaces between particle-bands. The comparisons between the numerical and experimental results show a good agreement and make the validity of the numerical results certain. MDPI 2020-05-31 /pmc/articles/PMC7344843/ /pubmed/32486500 http://dx.doi.org/10.3390/mi11060566 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Du, Jiayou Li, Long Zhuo, Qiuyi Wang, Ruijin Zhu, Zefei Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles |
title | Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles |
title_full | Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles |
title_fullStr | Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles |
title_full_unstemmed | Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles |
title_short | Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles |
title_sort | investigation on inertial sorter coupled with magnetophoretic effect for nonmagnetic microparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344843/ https://www.ncbi.nlm.nih.gov/pubmed/32486500 http://dx.doi.org/10.3390/mi11060566 |
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