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Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles

The sizes of most prokaryotic cells are several microns. It is very difficult to separate cells with similar sizes. A sorter with a contraction–expansion microchannel and applied magnetic field is designed to sort microparticles with diameters of 3, 4 and 5 microns. To evaluate the sorting efficienc...

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Detalles Bibliográficos
Autores principales: Du, Jiayou, Li, Long, Zhuo, Qiuyi, Wang, Ruijin, Zhu, Zefei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344843/
https://www.ncbi.nlm.nih.gov/pubmed/32486500
http://dx.doi.org/10.3390/mi11060566
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author Du, Jiayou
Li, Long
Zhuo, Qiuyi
Wang, Ruijin
Zhu, Zefei
author_facet Du, Jiayou
Li, Long
Zhuo, Qiuyi
Wang, Ruijin
Zhu, Zefei
author_sort Du, Jiayou
collection PubMed
description The sizes of most prokaryotic cells are several microns. It is very difficult to separate cells with similar sizes. A sorter with a contraction–expansion microchannel and applied magnetic field is designed to sort microparticles with diameters of 3, 4 and 5 microns. To evaluate the sorting efficiency of the designed sorter, numerical simulations for calculating the distributions of microparticles with similar sizes were carried out for various magnetic fields, inlet velocities, sheath flow ratios and structural parameters. The numerical results indicate that micro-particles with diameters of 3, 4 and 5 microns can be sorted efficiently in such a sorter within appropriate parameters. Furthermore, it is shown that a bigger particle size and more powerful magnetic field can result in a greater lateral migration of microparticles. The sorting efficiency of microparticles promotes a lower inlet velocity and greater sheath flow ratios. A smaller contraction–expansion ratio can induce a greater space between particle-bands. Finally, the micro particle image velocity (micro-PIV) experiments were conducted to obtain the bandwidths and spaces between particle-bands. The comparisons between the numerical and experimental results show a good agreement and make the validity of the numerical results certain.
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spelling pubmed-73448432020-07-09 Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles Du, Jiayou Li, Long Zhuo, Qiuyi Wang, Ruijin Zhu, Zefei Micromachines (Basel) Article The sizes of most prokaryotic cells are several microns. It is very difficult to separate cells with similar sizes. A sorter with a contraction–expansion microchannel and applied magnetic field is designed to sort microparticles with diameters of 3, 4 and 5 microns. To evaluate the sorting efficiency of the designed sorter, numerical simulations for calculating the distributions of microparticles with similar sizes were carried out for various magnetic fields, inlet velocities, sheath flow ratios and structural parameters. The numerical results indicate that micro-particles with diameters of 3, 4 and 5 microns can be sorted efficiently in such a sorter within appropriate parameters. Furthermore, it is shown that a bigger particle size and more powerful magnetic field can result in a greater lateral migration of microparticles. The sorting efficiency of microparticles promotes a lower inlet velocity and greater sheath flow ratios. A smaller contraction–expansion ratio can induce a greater space between particle-bands. Finally, the micro particle image velocity (micro-PIV) experiments were conducted to obtain the bandwidths and spaces between particle-bands. The comparisons between the numerical and experimental results show a good agreement and make the validity of the numerical results certain. MDPI 2020-05-31 /pmc/articles/PMC7344843/ /pubmed/32486500 http://dx.doi.org/10.3390/mi11060566 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Du, Jiayou
Li, Long
Zhuo, Qiuyi
Wang, Ruijin
Zhu, Zefei
Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles
title Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles
title_full Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles
title_fullStr Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles
title_full_unstemmed Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles
title_short Investigation on Inertial Sorter Coupled with Magnetophoretic Effect for Nonmagnetic Microparticles
title_sort investigation on inertial sorter coupled with magnetophoretic effect for nonmagnetic microparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344843/
https://www.ncbi.nlm.nih.gov/pubmed/32486500
http://dx.doi.org/10.3390/mi11060566
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