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Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)
The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks and spo...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344944/ https://www.ncbi.nlm.nih.gov/pubmed/32498448 http://dx.doi.org/10.3390/md18060297 |
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author | Nowacki, Krzysztof Stępniak, Izabela Langer, Enrico Tsurkan, Mikhail Wysokowski, Marcin Petrenko, Iaroslav Khrunyk, Yuliya Fursov, Andriy Bo, Marzia Bavestrello, Giorgio Joseph, Yvonne Ehrlich, Hermann |
author_facet | Nowacki, Krzysztof Stępniak, Izabela Langer, Enrico Tsurkan, Mikhail Wysokowski, Marcin Petrenko, Iaroslav Khrunyk, Yuliya Fursov, Andriy Bo, Marzia Bavestrello, Giorgio Joseph, Yvonne Ehrlich, Hermann |
author_sort | Nowacki, Krzysztof |
collection | PubMed |
description | The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks and sponges, there are only a few reports concerning its isolation from corals, and especially black corals (Antipatharia). In this work, we report the stepwise isolation and identification of chitin from Cirrhipathes sp. (Antipatharia, Antipathidae) for the first time. The proposed method, aiming at the extraction of the chitinous scaffold from the skeleton of black coral species, combined a well-known chemical treatment with in situ electrolysis, using a concentrated Na(2)SO(4) aqueous solution as the electrolyte. This novel method allows the isolation of α-chitin in the form of a microporous membrane-like material. Moreover, the extracted chitinous scaffold, with a well-preserved, unique pore distribution, has been extracted in an astoundingly short time (12 h) compared to the earlier reported attempts at chitin isolation from Antipatharia corals. |
format | Online Article Text |
id | pubmed-7344944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73449442020-07-09 Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia) Nowacki, Krzysztof Stępniak, Izabela Langer, Enrico Tsurkan, Mikhail Wysokowski, Marcin Petrenko, Iaroslav Khrunyk, Yuliya Fursov, Andriy Bo, Marzia Bavestrello, Giorgio Joseph, Yvonne Ehrlich, Hermann Mar Drugs Article The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks and sponges, there are only a few reports concerning its isolation from corals, and especially black corals (Antipatharia). In this work, we report the stepwise isolation and identification of chitin from Cirrhipathes sp. (Antipatharia, Antipathidae) for the first time. The proposed method, aiming at the extraction of the chitinous scaffold from the skeleton of black coral species, combined a well-known chemical treatment with in situ electrolysis, using a concentrated Na(2)SO(4) aqueous solution as the electrolyte. This novel method allows the isolation of α-chitin in the form of a microporous membrane-like material. Moreover, the extracted chitinous scaffold, with a well-preserved, unique pore distribution, has been extracted in an astoundingly short time (12 h) compared to the earlier reported attempts at chitin isolation from Antipatharia corals. MDPI 2020-06-02 /pmc/articles/PMC7344944/ /pubmed/32498448 http://dx.doi.org/10.3390/md18060297 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nowacki, Krzysztof Stępniak, Izabela Langer, Enrico Tsurkan, Mikhail Wysokowski, Marcin Petrenko, Iaroslav Khrunyk, Yuliya Fursov, Andriy Bo, Marzia Bavestrello, Giorgio Joseph, Yvonne Ehrlich, Hermann Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia) |
title | Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia) |
title_full | Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia) |
title_fullStr | Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia) |
title_full_unstemmed | Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia) |
title_short | Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia) |
title_sort | electrochemical approach for isolation of chitin from the skeleton of the black coral cirrhipathes sp. (antipatharia) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344944/ https://www.ncbi.nlm.nih.gov/pubmed/32498448 http://dx.doi.org/10.3390/md18060297 |
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