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Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme

As a supramolecular micromachine with information flow, a giant vesicle (GV)-based artificial cell that exhibits a linked proliferation between GV reproduction and internal DNA amplification has been explored in this study. The linked proliferation is controlled by a complex consisting of GV membran...

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Autores principales: Matsuo, Muneyuki, Hirata, Yuiko, Kurihara, Kensuke, Toyota, Taro, Miura, Toru, Suzuki, Kentaro, Sugawara, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344958/
https://www.ncbi.nlm.nih.gov/pubmed/32580457
http://dx.doi.org/10.3390/mi11060606
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author Matsuo, Muneyuki
Hirata, Yuiko
Kurihara, Kensuke
Toyota, Taro
Miura, Toru
Suzuki, Kentaro
Sugawara, Tadashi
author_facet Matsuo, Muneyuki
Hirata, Yuiko
Kurihara, Kensuke
Toyota, Taro
Miura, Toru
Suzuki, Kentaro
Sugawara, Tadashi
author_sort Matsuo, Muneyuki
collection PubMed
description As a supramolecular micromachine with information flow, a giant vesicle (GV)-based artificial cell that exhibits a linked proliferation between GV reproduction and internal DNA amplification has been explored in this study. The linked proliferation is controlled by a complex consisting of GV membrane-intruded DNA with acidic amphiphilic catalysts, working overall as a lipo-deoxyribozyme. Here, we investigated how a GV-based artificial cell containing this lipo-deoxyribozyme responds to diverse external and internal environments, changing its proliferative dynamics. We observed morphological changes (phenotypic expression) in GVs induced by the addition of membrane precursors with different intervals of addition (starvation periods). First, we focused on a new phenotype, the “multiple tubulated” form, which emerged after a long starvation period. Compared to other forms, the multiple tubulated form is characterized by a larger membrane surface with a heavily cationic charge. A second consideration is the effect of the chain length of encapsulated DNA on competitive proliferation. The competitive proliferation among three different species of artificial cells containing different lengths of DNA was investigated. The results clearly showed a distinct intervention in the proliferation dynamics of the artificial cells with each other. In this sense, our GV-based artificial cell can be regarded as an intelligent supramolecular machine responding to external and internal environments, providing a new concept for developing molecular machines and robotics.
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spelling pubmed-73449582020-07-09 Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme Matsuo, Muneyuki Hirata, Yuiko Kurihara, Kensuke Toyota, Taro Miura, Toru Suzuki, Kentaro Sugawara, Tadashi Micromachines (Basel) Article As a supramolecular micromachine with information flow, a giant vesicle (GV)-based artificial cell that exhibits a linked proliferation between GV reproduction and internal DNA amplification has been explored in this study. The linked proliferation is controlled by a complex consisting of GV membrane-intruded DNA with acidic amphiphilic catalysts, working overall as a lipo-deoxyribozyme. Here, we investigated how a GV-based artificial cell containing this lipo-deoxyribozyme responds to diverse external and internal environments, changing its proliferative dynamics. We observed morphological changes (phenotypic expression) in GVs induced by the addition of membrane precursors with different intervals of addition (starvation periods). First, we focused on a new phenotype, the “multiple tubulated” form, which emerged after a long starvation period. Compared to other forms, the multiple tubulated form is characterized by a larger membrane surface with a heavily cationic charge. A second consideration is the effect of the chain length of encapsulated DNA on competitive proliferation. The competitive proliferation among three different species of artificial cells containing different lengths of DNA was investigated. The results clearly showed a distinct intervention in the proliferation dynamics of the artificial cells with each other. In this sense, our GV-based artificial cell can be regarded as an intelligent supramolecular machine responding to external and internal environments, providing a new concept for developing molecular machines and robotics. MDPI 2020-06-22 /pmc/articles/PMC7344958/ /pubmed/32580457 http://dx.doi.org/10.3390/mi11060606 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matsuo, Muneyuki
Hirata, Yuiko
Kurihara, Kensuke
Toyota, Taro
Miura, Toru
Suzuki, Kentaro
Sugawara, Tadashi
Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme
title Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme
title_full Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme
title_fullStr Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme
title_full_unstemmed Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme
title_short Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme
title_sort environment-sensitive intelligent self-reproducing artificial cell with a modification-active lipo-deoxyribozyme
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344958/
https://www.ncbi.nlm.nih.gov/pubmed/32580457
http://dx.doi.org/10.3390/mi11060606
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