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Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum)

Flax for oil seed is a crop of increasing popularity, but its cultivation needs technical improvement. Important agronomic traits such as productivity and resistance to stresses are to be regarded as the result of the combined responses of individual organs and their inter-communication. Ultimately,...

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Autores principales: Pontarin, Nicole, Molinié, Roland, Mathiron, David, Tchoumtchoua, Job, Bassard, Solène, Gagneul, David, Thiombiano, Benjamin, Demailly, Hervé, Fontaine, Jean-Xavier, Guillot, Xavier, Sarazin, Vivien, Quéro, Anthony, Mesnard, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345097/
https://www.ncbi.nlm.nih.gov/pubmed/32466546
http://dx.doi.org/10.3390/metabo10060218
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author Pontarin, Nicole
Molinié, Roland
Mathiron, David
Tchoumtchoua, Job
Bassard, Solène
Gagneul, David
Thiombiano, Benjamin
Demailly, Hervé
Fontaine, Jean-Xavier
Guillot, Xavier
Sarazin, Vivien
Quéro, Anthony
Mesnard, François
author_facet Pontarin, Nicole
Molinié, Roland
Mathiron, David
Tchoumtchoua, Job
Bassard, Solène
Gagneul, David
Thiombiano, Benjamin
Demailly, Hervé
Fontaine, Jean-Xavier
Guillot, Xavier
Sarazin, Vivien
Quéro, Anthony
Mesnard, François
author_sort Pontarin, Nicole
collection PubMed
description Flax for oil seed is a crop of increasing popularity, but its cultivation needs technical improvement. Important agronomic traits such as productivity and resistance to stresses are to be regarded as the result of the combined responses of individual organs and their inter-communication. Ultimately, these responses directly reflect the metabolic profile at the cellular level. Above ground, the complexity of the plant phenotype is governed by leaves at different developmental stages, and their ability to synthesise and exchange metabolites. In this study, the metabolic profile of differently-developed leaves was used firstly to discriminate flax leaf developmental stages, and secondly to analyse the allocation of the metabolites within and between leaves. For this purpose, the concentration of 52 metabolites, both primary and specialized, was followed by gas chromatography (GC-) and liquid chromatography coupled to mass spectrometry (LC-MS) in alternate pairs of flax leaves. On the basis of their metabolic content, three populations of leaves in different growth stages could be distinguished. Primary and specialized metabolites showed characteristic distribution patterns, and compounds similarly evolving with leaf age could be grouped by the aid of the Kohonen self-organising map (SOM) algorithm. Ultimately, visualisation of the correlations between metabolites via hierarchical cluster analysis (HCA) allowed the assessment of the metabolic fluxes characterising different leaf developmental stages, and the investigation of the relationships between primary and specialized metabolites.
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spelling pubmed-73450972020-07-09 Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum) Pontarin, Nicole Molinié, Roland Mathiron, David Tchoumtchoua, Job Bassard, Solène Gagneul, David Thiombiano, Benjamin Demailly, Hervé Fontaine, Jean-Xavier Guillot, Xavier Sarazin, Vivien Quéro, Anthony Mesnard, François Metabolites Article Flax for oil seed is a crop of increasing popularity, but its cultivation needs technical improvement. Important agronomic traits such as productivity and resistance to stresses are to be regarded as the result of the combined responses of individual organs and their inter-communication. Ultimately, these responses directly reflect the metabolic profile at the cellular level. Above ground, the complexity of the plant phenotype is governed by leaves at different developmental stages, and their ability to synthesise and exchange metabolites. In this study, the metabolic profile of differently-developed leaves was used firstly to discriminate flax leaf developmental stages, and secondly to analyse the allocation of the metabolites within and between leaves. For this purpose, the concentration of 52 metabolites, both primary and specialized, was followed by gas chromatography (GC-) and liquid chromatography coupled to mass spectrometry (LC-MS) in alternate pairs of flax leaves. On the basis of their metabolic content, three populations of leaves in different growth stages could be distinguished. Primary and specialized metabolites showed characteristic distribution patterns, and compounds similarly evolving with leaf age could be grouped by the aid of the Kohonen self-organising map (SOM) algorithm. Ultimately, visualisation of the correlations between metabolites via hierarchical cluster analysis (HCA) allowed the assessment of the metabolic fluxes characterising different leaf developmental stages, and the investigation of the relationships between primary and specialized metabolites. MDPI 2020-05-26 /pmc/articles/PMC7345097/ /pubmed/32466546 http://dx.doi.org/10.3390/metabo10060218 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pontarin, Nicole
Molinié, Roland
Mathiron, David
Tchoumtchoua, Job
Bassard, Solène
Gagneul, David
Thiombiano, Benjamin
Demailly, Hervé
Fontaine, Jean-Xavier
Guillot, Xavier
Sarazin, Vivien
Quéro, Anthony
Mesnard, François
Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum)
title Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum)
title_full Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum)
title_fullStr Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum)
title_full_unstemmed Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum)
title_short Age-Dependent Metabolic Profiles Unravel the Metabolic Relationships within and between Flax Leaves (Linum usitatissimum)
title_sort age-dependent metabolic profiles unravel the metabolic relationships within and between flax leaves (linum usitatissimum)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345097/
https://www.ncbi.nlm.nih.gov/pubmed/32466546
http://dx.doi.org/10.3390/metabo10060218
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