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Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging

3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D im...

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Detalles Bibliográficos
Autores principales: Lee, Dongwoo, Kim, Jihye, Song, Eunjoo, Jeong, Ji-Young, Jeon, Eun-chae, Kim, Pilhan, Lee, Wonhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345240/
https://www.ncbi.nlm.nih.gov/pubmed/32532128
http://dx.doi.org/10.3390/mi11060582
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author Lee, Dongwoo
Kim, Jihye
Song, Eunjoo
Jeong, Ji-Young
Jeon, Eun-chae
Kim, Pilhan
Lee, Wonhee
author_facet Lee, Dongwoo
Kim, Jihye
Song, Eunjoo
Jeong, Ji-Young
Jeon, Eun-chae
Kim, Pilhan
Lee, Wonhee
author_sort Lee, Dongwoo
collection PubMed
description 3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures.
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spelling pubmed-73452402020-07-09 Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging Lee, Dongwoo Kim, Jihye Song, Eunjoo Jeong, Ji-Young Jeon, Eun-chae Kim, Pilhan Lee, Wonhee Micromachines (Basel) Article 3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures. MDPI 2020-06-10 /pmc/articles/PMC7345240/ /pubmed/32532128 http://dx.doi.org/10.3390/mi11060582 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Dongwoo
Kim, Jihye
Song, Eunjoo
Jeong, Ji-Young
Jeon, Eun-chae
Kim, Pilhan
Lee, Wonhee
Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging
title Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging
title_full Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging
title_fullStr Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging
title_full_unstemmed Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging
title_short Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging
title_sort micromirror-embedded coverslip assembly for bidirectional microscopic imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345240/
https://www.ncbi.nlm.nih.gov/pubmed/32532128
http://dx.doi.org/10.3390/mi11060582
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