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Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance

In West Africa, Aedes aegypti remains the major vector of dengue virus. Since 2013, dengue fever has been reemerging in Burkina Faso with annual outbreaks, thus becoming a major public health problem. Its control relies on vector control, which is unfortunately facing the problem of insecticide resi...

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Autores principales: Namountougou, Moussa, Soma, Dieudonné Diloma, Balboné, Mahamoudou, Kaboré, Didier Alexandre, Kientega, Mahamadi, Hien, Aristide, Coulibaly, Ahmed, Ouattara, Parfait Eric, Meda, Benson Georges, Drabo, Samuel, Koala, Lassane, Nignan, Charles, Kagoné, Thérèse, Diabaté, Abdoulaye, Fournet, Florence, Gnankiné, Olivier, Dabiré, Roch Kounbobr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345320/
https://www.ncbi.nlm.nih.gov/pubmed/32471266
http://dx.doi.org/10.3390/tropicalmed5020084
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author Namountougou, Moussa
Soma, Dieudonné Diloma
Balboné, Mahamoudou
Kaboré, Didier Alexandre
Kientega, Mahamadi
Hien, Aristide
Coulibaly, Ahmed
Ouattara, Parfait Eric
Meda, Benson Georges
Drabo, Samuel
Koala, Lassane
Nignan, Charles
Kagoné, Thérèse
Diabaté, Abdoulaye
Fournet, Florence
Gnankiné, Olivier
Dabiré, Roch Kounbobr
author_facet Namountougou, Moussa
Soma, Dieudonné Diloma
Balboné, Mahamoudou
Kaboré, Didier Alexandre
Kientega, Mahamadi
Hien, Aristide
Coulibaly, Ahmed
Ouattara, Parfait Eric
Meda, Benson Georges
Drabo, Samuel
Koala, Lassane
Nignan, Charles
Kagoné, Thérèse
Diabaté, Abdoulaye
Fournet, Florence
Gnankiné, Olivier
Dabiré, Roch Kounbobr
author_sort Namountougou, Moussa
collection PubMed
description In West Africa, Aedes aegypti remains the major vector of dengue virus. Since 2013, dengue fever has been reemerging in Burkina Faso with annual outbreaks, thus becoming a major public health problem. Its control relies on vector control, which is unfortunately facing the problem of insecticide resistance. At the time of this study, although data on phenotypic resistance were available, information related to the metabolic resistance in Aedes populations from Burkina Faso remained very scarce. Here, we assessed the phenotypic and the metabolic resistance of Ae. aegypti populations sampled from the two main urban areas (Ouagadougou and Bobo-Dioulasso) of Burkina Faso. Insecticide susceptibility bioassays to chlorpyriphos-methyl 0.4%, bendiocarb 0.1% and deltamethrin 0.05% were performed on natural populations of Ae. aegypti using the WHO protocol. The activity of enzymes involved in the rapid detoxification of insecticides, especially non-specific esterases, oxidases (cytochrome P450) and glutathione-S-transferases, was measured on individual mosquitos. The mortality rates for deltamethrin 0.05% were low and ranged from 20.72% to 89.62% in the Bobo-Dioulasso and Ouagadougou sites, respectively. When bendiocarb 0.1% was tested, the mortality rates ranged from 7.73% to 71.23%. Interestingly, in the two urban areas, mosquitoes were found to be fully susceptible to chlorpyriphos-methyl 0.4%. Elevated activity of non-specific esterases and glutathione-S-transferases was reported, suggesting multiple resistance mechanisms involved in Ae. aegypti populations from Bobo-Dioulasso and Ouagadougou (including cytochrome P450). This update to the insecticide resistance status within Ae. aegypti populations in the two biggest cities is important to better plan dengue vectors control in the country and provides valuable information for improving vector control strategies in Burkina Faso, West Africa.
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spelling pubmed-73453202020-07-09 Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance Namountougou, Moussa Soma, Dieudonné Diloma Balboné, Mahamoudou Kaboré, Didier Alexandre Kientega, Mahamadi Hien, Aristide Coulibaly, Ahmed Ouattara, Parfait Eric Meda, Benson Georges Drabo, Samuel Koala, Lassane Nignan, Charles Kagoné, Thérèse Diabaté, Abdoulaye Fournet, Florence Gnankiné, Olivier Dabiré, Roch Kounbobr Trop Med Infect Dis Article In West Africa, Aedes aegypti remains the major vector of dengue virus. Since 2013, dengue fever has been reemerging in Burkina Faso with annual outbreaks, thus becoming a major public health problem. Its control relies on vector control, which is unfortunately facing the problem of insecticide resistance. At the time of this study, although data on phenotypic resistance were available, information related to the metabolic resistance in Aedes populations from Burkina Faso remained very scarce. Here, we assessed the phenotypic and the metabolic resistance of Ae. aegypti populations sampled from the two main urban areas (Ouagadougou and Bobo-Dioulasso) of Burkina Faso. Insecticide susceptibility bioassays to chlorpyriphos-methyl 0.4%, bendiocarb 0.1% and deltamethrin 0.05% were performed on natural populations of Ae. aegypti using the WHO protocol. The activity of enzymes involved in the rapid detoxification of insecticides, especially non-specific esterases, oxidases (cytochrome P450) and glutathione-S-transferases, was measured on individual mosquitos. The mortality rates for deltamethrin 0.05% were low and ranged from 20.72% to 89.62% in the Bobo-Dioulasso and Ouagadougou sites, respectively. When bendiocarb 0.1% was tested, the mortality rates ranged from 7.73% to 71.23%. Interestingly, in the two urban areas, mosquitoes were found to be fully susceptible to chlorpyriphos-methyl 0.4%. Elevated activity of non-specific esterases and glutathione-S-transferases was reported, suggesting multiple resistance mechanisms involved in Ae. aegypti populations from Bobo-Dioulasso and Ouagadougou (including cytochrome P450). This update to the insecticide resistance status within Ae. aegypti populations in the two biggest cities is important to better plan dengue vectors control in the country and provides valuable information for improving vector control strategies in Burkina Faso, West Africa. MDPI 2020-05-27 /pmc/articles/PMC7345320/ /pubmed/32471266 http://dx.doi.org/10.3390/tropicalmed5020084 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Namountougou, Moussa
Soma, Dieudonné Diloma
Balboné, Mahamoudou
Kaboré, Didier Alexandre
Kientega, Mahamadi
Hien, Aristide
Coulibaly, Ahmed
Ouattara, Parfait Eric
Meda, Benson Georges
Drabo, Samuel
Koala, Lassane
Nignan, Charles
Kagoné, Thérèse
Diabaté, Abdoulaye
Fournet, Florence
Gnankiné, Olivier
Dabiré, Roch Kounbobr
Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance
title Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance
title_full Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance
title_fullStr Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance
title_full_unstemmed Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance
title_short Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance
title_sort monitoring insecticide susceptibility in aedes aegypti populations from the two biggest cities, ouagadougou and bobo-dioulasso, in burkina faso: implication of metabolic resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345320/
https://www.ncbi.nlm.nih.gov/pubmed/32471266
http://dx.doi.org/10.3390/tropicalmed5020084
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