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Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance
In West Africa, Aedes aegypti remains the major vector of dengue virus. Since 2013, dengue fever has been reemerging in Burkina Faso with annual outbreaks, thus becoming a major public health problem. Its control relies on vector control, which is unfortunately facing the problem of insecticide resi...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345320/ https://www.ncbi.nlm.nih.gov/pubmed/32471266 http://dx.doi.org/10.3390/tropicalmed5020084 |
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author | Namountougou, Moussa Soma, Dieudonné Diloma Balboné, Mahamoudou Kaboré, Didier Alexandre Kientega, Mahamadi Hien, Aristide Coulibaly, Ahmed Ouattara, Parfait Eric Meda, Benson Georges Drabo, Samuel Koala, Lassane Nignan, Charles Kagoné, Thérèse Diabaté, Abdoulaye Fournet, Florence Gnankiné, Olivier Dabiré, Roch Kounbobr |
author_facet | Namountougou, Moussa Soma, Dieudonné Diloma Balboné, Mahamoudou Kaboré, Didier Alexandre Kientega, Mahamadi Hien, Aristide Coulibaly, Ahmed Ouattara, Parfait Eric Meda, Benson Georges Drabo, Samuel Koala, Lassane Nignan, Charles Kagoné, Thérèse Diabaté, Abdoulaye Fournet, Florence Gnankiné, Olivier Dabiré, Roch Kounbobr |
author_sort | Namountougou, Moussa |
collection | PubMed |
description | In West Africa, Aedes aegypti remains the major vector of dengue virus. Since 2013, dengue fever has been reemerging in Burkina Faso with annual outbreaks, thus becoming a major public health problem. Its control relies on vector control, which is unfortunately facing the problem of insecticide resistance. At the time of this study, although data on phenotypic resistance were available, information related to the metabolic resistance in Aedes populations from Burkina Faso remained very scarce. Here, we assessed the phenotypic and the metabolic resistance of Ae. aegypti populations sampled from the two main urban areas (Ouagadougou and Bobo-Dioulasso) of Burkina Faso. Insecticide susceptibility bioassays to chlorpyriphos-methyl 0.4%, bendiocarb 0.1% and deltamethrin 0.05% were performed on natural populations of Ae. aegypti using the WHO protocol. The activity of enzymes involved in the rapid detoxification of insecticides, especially non-specific esterases, oxidases (cytochrome P450) and glutathione-S-transferases, was measured on individual mosquitos. The mortality rates for deltamethrin 0.05% were low and ranged from 20.72% to 89.62% in the Bobo-Dioulasso and Ouagadougou sites, respectively. When bendiocarb 0.1% was tested, the mortality rates ranged from 7.73% to 71.23%. Interestingly, in the two urban areas, mosquitoes were found to be fully susceptible to chlorpyriphos-methyl 0.4%. Elevated activity of non-specific esterases and glutathione-S-transferases was reported, suggesting multiple resistance mechanisms involved in Ae. aegypti populations from Bobo-Dioulasso and Ouagadougou (including cytochrome P450). This update to the insecticide resistance status within Ae. aegypti populations in the two biggest cities is important to better plan dengue vectors control in the country and provides valuable information for improving vector control strategies in Burkina Faso, West Africa. |
format | Online Article Text |
id | pubmed-7345320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73453202020-07-09 Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance Namountougou, Moussa Soma, Dieudonné Diloma Balboné, Mahamoudou Kaboré, Didier Alexandre Kientega, Mahamadi Hien, Aristide Coulibaly, Ahmed Ouattara, Parfait Eric Meda, Benson Georges Drabo, Samuel Koala, Lassane Nignan, Charles Kagoné, Thérèse Diabaté, Abdoulaye Fournet, Florence Gnankiné, Olivier Dabiré, Roch Kounbobr Trop Med Infect Dis Article In West Africa, Aedes aegypti remains the major vector of dengue virus. Since 2013, dengue fever has been reemerging in Burkina Faso with annual outbreaks, thus becoming a major public health problem. Its control relies on vector control, which is unfortunately facing the problem of insecticide resistance. At the time of this study, although data on phenotypic resistance were available, information related to the metabolic resistance in Aedes populations from Burkina Faso remained very scarce. Here, we assessed the phenotypic and the metabolic resistance of Ae. aegypti populations sampled from the two main urban areas (Ouagadougou and Bobo-Dioulasso) of Burkina Faso. Insecticide susceptibility bioassays to chlorpyriphos-methyl 0.4%, bendiocarb 0.1% and deltamethrin 0.05% were performed on natural populations of Ae. aegypti using the WHO protocol. The activity of enzymes involved in the rapid detoxification of insecticides, especially non-specific esterases, oxidases (cytochrome P450) and glutathione-S-transferases, was measured on individual mosquitos. The mortality rates for deltamethrin 0.05% were low and ranged from 20.72% to 89.62% in the Bobo-Dioulasso and Ouagadougou sites, respectively. When bendiocarb 0.1% was tested, the mortality rates ranged from 7.73% to 71.23%. Interestingly, in the two urban areas, mosquitoes were found to be fully susceptible to chlorpyriphos-methyl 0.4%. Elevated activity of non-specific esterases and glutathione-S-transferases was reported, suggesting multiple resistance mechanisms involved in Ae. aegypti populations from Bobo-Dioulasso and Ouagadougou (including cytochrome P450). This update to the insecticide resistance status within Ae. aegypti populations in the two biggest cities is important to better plan dengue vectors control in the country and provides valuable information for improving vector control strategies in Burkina Faso, West Africa. MDPI 2020-05-27 /pmc/articles/PMC7345320/ /pubmed/32471266 http://dx.doi.org/10.3390/tropicalmed5020084 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Namountougou, Moussa Soma, Dieudonné Diloma Balboné, Mahamoudou Kaboré, Didier Alexandre Kientega, Mahamadi Hien, Aristide Coulibaly, Ahmed Ouattara, Parfait Eric Meda, Benson Georges Drabo, Samuel Koala, Lassane Nignan, Charles Kagoné, Thérèse Diabaté, Abdoulaye Fournet, Florence Gnankiné, Olivier Dabiré, Roch Kounbobr Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance |
title | Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance |
title_full | Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance |
title_fullStr | Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance |
title_full_unstemmed | Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance |
title_short | Monitoring Insecticide Susceptibility in Aedes Aegypti Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance |
title_sort | monitoring insecticide susceptibility in aedes aegypti populations from the two biggest cities, ouagadougou and bobo-dioulasso, in burkina faso: implication of metabolic resistance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345320/ https://www.ncbi.nlm.nih.gov/pubmed/32471266 http://dx.doi.org/10.3390/tropicalmed5020084 |
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