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Phosphoinositide Lipids in Ocular Tissues
Inositol phospholipids play an important role in cell physiology. The inositol head groups are reversibly phosphorylated to produce seven distinct phosphorylated inositides, commonly referred to as phosphoinositides (PIs). These seven PIs are dynamically interconverted from one PI to another by the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345453/ https://www.ncbi.nlm.nih.gov/pubmed/32545642 http://dx.doi.org/10.3390/biology9060125 |
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author | Rajala, Ammaji McCauley, Austin Brush, Richard S. Nguyen, Khuong Rajala, Raju V.S. |
author_facet | Rajala, Ammaji McCauley, Austin Brush, Richard S. Nguyen, Khuong Rajala, Raju V.S. |
author_sort | Rajala, Ammaji |
collection | PubMed |
description | Inositol phospholipids play an important role in cell physiology. The inositol head groups are reversibly phosphorylated to produce seven distinct phosphorylated inositides, commonly referred to as phosphoinositides (PIs). These seven PIs are dynamically interconverted from one PI to another by the action of PI kinases and PI phosphatases. The PI signals regulate a wide variety of cellular functions, including organelle distinction, vesicular transport, cytoskeletal organization, nuclear events, regulation of ion channels, cell signaling, and host–pathogen interactions. Most of the studies of PIs in ocular tissues are based on the PI enzymes and PI phosphatases. In this study, we examined the PI levels in the cornea, retinal pigment epithelium (RPE), and retina using PI-binding protein as probes. We have examined the lipids PI(3)P, PI(4)P, PI(3,4)P(2), PI(4,5)P(2), and PI(3,4,5)P(3), and each is present in the cornea, RPE, and retina. Alterations in the levels of these PIs in mouse models of retinal disease and corneal infections have been reported, and the results of our study will help in the management of anomalous phosphoinositide metabolism in ocular tissues. |
format | Online Article Text |
id | pubmed-7345453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73454532020-07-09 Phosphoinositide Lipids in Ocular Tissues Rajala, Ammaji McCauley, Austin Brush, Richard S. Nguyen, Khuong Rajala, Raju V.S. Biology (Basel) Article Inositol phospholipids play an important role in cell physiology. The inositol head groups are reversibly phosphorylated to produce seven distinct phosphorylated inositides, commonly referred to as phosphoinositides (PIs). These seven PIs are dynamically interconverted from one PI to another by the action of PI kinases and PI phosphatases. The PI signals regulate a wide variety of cellular functions, including organelle distinction, vesicular transport, cytoskeletal organization, nuclear events, regulation of ion channels, cell signaling, and host–pathogen interactions. Most of the studies of PIs in ocular tissues are based on the PI enzymes and PI phosphatases. In this study, we examined the PI levels in the cornea, retinal pigment epithelium (RPE), and retina using PI-binding protein as probes. We have examined the lipids PI(3)P, PI(4)P, PI(3,4)P(2), PI(4,5)P(2), and PI(3,4,5)P(3), and each is present in the cornea, RPE, and retina. Alterations in the levels of these PIs in mouse models of retinal disease and corneal infections have been reported, and the results of our study will help in the management of anomalous phosphoinositide metabolism in ocular tissues. MDPI 2020-06-12 /pmc/articles/PMC7345453/ /pubmed/32545642 http://dx.doi.org/10.3390/biology9060125 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rajala, Ammaji McCauley, Austin Brush, Richard S. Nguyen, Khuong Rajala, Raju V.S. Phosphoinositide Lipids in Ocular Tissues |
title | Phosphoinositide Lipids in Ocular Tissues |
title_full | Phosphoinositide Lipids in Ocular Tissues |
title_fullStr | Phosphoinositide Lipids in Ocular Tissues |
title_full_unstemmed | Phosphoinositide Lipids in Ocular Tissues |
title_short | Phosphoinositide Lipids in Ocular Tissues |
title_sort | phosphoinositide lipids in ocular tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345453/ https://www.ncbi.nlm.nih.gov/pubmed/32545642 http://dx.doi.org/10.3390/biology9060125 |
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