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A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis
In this report, we describe the development and testing of a new coated plate which improves the sensitivity and accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS). The coated plate was covered with a thin layer of hydrophobic silicon dioxide, whi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345610/ https://www.ncbi.nlm.nih.gov/pubmed/32685536 http://dx.doi.org/10.1155/2020/8597217 |
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author | Huang, He Sun, Lei He, Haiyang Xia, Tianbao |
author_facet | Huang, He Sun, Lei He, Haiyang Xia, Tianbao |
author_sort | Huang, He |
collection | PubMed |
description | In this report, we describe the development and testing of a new coated plate which improves the sensitivity and accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS). The coated plate was covered with a thin layer of hydrophobic silicon dioxide, which enabled sample enrichment due to the water repellent nature of the silicon dioxide surface. Sensitivity and required laser strengths were tested using peptide standards, with the results that these coated plates required lower laser power and showed increased sensitivity than that of common plates. Accuracy was tested using bacteria, saliva, and serum samples. The coated plates showed significantly increased degrees of accuracy through their capacity to reduce mass shift. The importance and necessity of accuracy analysis in the assessment of new sample plates, which is rarely described in other papers, is also discussed. |
format | Online Article Text |
id | pubmed-7345610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-73456102020-07-17 A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis Huang, He Sun, Lei He, Haiyang Xia, Tianbao Biomed Res Int Research Article In this report, we describe the development and testing of a new coated plate which improves the sensitivity and accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS). The coated plate was covered with a thin layer of hydrophobic silicon dioxide, which enabled sample enrichment due to the water repellent nature of the silicon dioxide surface. Sensitivity and required laser strengths were tested using peptide standards, with the results that these coated plates required lower laser power and showed increased sensitivity than that of common plates. Accuracy was tested using bacteria, saliva, and serum samples. The coated plates showed significantly increased degrees of accuracy through their capacity to reduce mass shift. The importance and necessity of accuracy analysis in the assessment of new sample plates, which is rarely described in other papers, is also discussed. Hindawi 2020-06-30 /pmc/articles/PMC7345610/ /pubmed/32685536 http://dx.doi.org/10.1155/2020/8597217 Text en Copyright © 2020 He Huang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Huang, He Sun, Lei He, Haiyang Xia, Tianbao A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis |
title | A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis |
title_full | A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis |
title_fullStr | A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis |
title_full_unstemmed | A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis |
title_short | A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis |
title_sort | new silicon dioxide-coated maldi-tof sample plate for peptide analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345610/ https://www.ncbi.nlm.nih.gov/pubmed/32685536 http://dx.doi.org/10.1155/2020/8597217 |
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