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Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach
Given their photoluminescent character, portable quantum dot readers are often sophisticated and relatively expensive. In response, we engineered a “do it yourself” fluorescence reader employing paper materials and a mid-range smartphone camera. Black paperboard facilitated a versatile, lightweight...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345677/ https://www.ncbi.nlm.nih.gov/pubmed/32498366 http://dx.doi.org/10.3390/bios10060060 |
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author | Ireta-Muñoz, Laura Alejandra Morales-Narváez, Eden |
author_facet | Ireta-Muñoz, Laura Alejandra Morales-Narváez, Eden |
author_sort | Ireta-Muñoz, Laura Alejandra |
collection | PubMed |
description | Given their photoluminescent character, portable quantum dot readers are often sophisticated and relatively expensive. In response, we engineered a “do it yourself” fluorescence reader employing paper materials and a mid-range smartphone camera. Black paperboard facilitated a versatile, lightweight and foldable case; whereas cellophane paper was observed to behave as a simple, yet effective, optical bandpass filter leading to an advantageous device for the quantitative interrogation of quantum dot nanocrystals concentrations (from 2.5 to 20 nM), which are suitable for optical point-of-care biosensing. The streptavidin-coated nanocrystals employed are commercially available and the developed reader was benchmarked with a standard portable quantum dot reader, thereby demonstrating advantages in terms of cost and linear analytical range. |
format | Online Article Text |
id | pubmed-7345677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73456772020-07-09 Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach Ireta-Muñoz, Laura Alejandra Morales-Narváez, Eden Biosensors (Basel) Communication Given their photoluminescent character, portable quantum dot readers are often sophisticated and relatively expensive. In response, we engineered a “do it yourself” fluorescence reader employing paper materials and a mid-range smartphone camera. Black paperboard facilitated a versatile, lightweight and foldable case; whereas cellophane paper was observed to behave as a simple, yet effective, optical bandpass filter leading to an advantageous device for the quantitative interrogation of quantum dot nanocrystals concentrations (from 2.5 to 20 nM), which are suitable for optical point-of-care biosensing. The streptavidin-coated nanocrystals employed are commercially available and the developed reader was benchmarked with a standard portable quantum dot reader, thereby demonstrating advantages in terms of cost and linear analytical range. MDPI 2020-06-02 /pmc/articles/PMC7345677/ /pubmed/32498366 http://dx.doi.org/10.3390/bios10060060 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ireta-Muñoz, Laura Alejandra Morales-Narváez, Eden Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach |
title | Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach |
title_full | Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach |
title_fullStr | Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach |
title_full_unstemmed | Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach |
title_short | Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach |
title_sort | smartphone and paper-based fluorescence reader: a do it yourself approach |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345677/ https://www.ncbi.nlm.nih.gov/pubmed/32498366 http://dx.doi.org/10.3390/bios10060060 |
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