Cargando…

Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach

Given their photoluminescent character, portable quantum dot readers are often sophisticated and relatively expensive. In response, we engineered a “do it yourself” fluorescence reader employing paper materials and a mid-range smartphone camera. Black paperboard facilitated a versatile, lightweight...

Descripción completa

Detalles Bibliográficos
Autores principales: Ireta-Muñoz, Laura Alejandra, Morales-Narváez, Eden
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345677/
https://www.ncbi.nlm.nih.gov/pubmed/32498366
http://dx.doi.org/10.3390/bios10060060
_version_ 1783556239260647424
author Ireta-Muñoz, Laura Alejandra
Morales-Narváez, Eden
author_facet Ireta-Muñoz, Laura Alejandra
Morales-Narváez, Eden
author_sort Ireta-Muñoz, Laura Alejandra
collection PubMed
description Given their photoluminescent character, portable quantum dot readers are often sophisticated and relatively expensive. In response, we engineered a “do it yourself” fluorescence reader employing paper materials and a mid-range smartphone camera. Black paperboard facilitated a versatile, lightweight and foldable case; whereas cellophane paper was observed to behave as a simple, yet effective, optical bandpass filter leading to an advantageous device for the quantitative interrogation of quantum dot nanocrystals concentrations (from 2.5 to 20 nM), which are suitable for optical point-of-care biosensing. The streptavidin-coated nanocrystals employed are commercially available and the developed reader was benchmarked with a standard portable quantum dot reader, thereby demonstrating advantages in terms of cost and linear analytical range.
format Online
Article
Text
id pubmed-7345677
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73456772020-07-09 Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach Ireta-Muñoz, Laura Alejandra Morales-Narváez, Eden Biosensors (Basel) Communication Given their photoluminescent character, portable quantum dot readers are often sophisticated and relatively expensive. In response, we engineered a “do it yourself” fluorescence reader employing paper materials and a mid-range smartphone camera. Black paperboard facilitated a versatile, lightweight and foldable case; whereas cellophane paper was observed to behave as a simple, yet effective, optical bandpass filter leading to an advantageous device for the quantitative interrogation of quantum dot nanocrystals concentrations (from 2.5 to 20 nM), which are suitable for optical point-of-care biosensing. The streptavidin-coated nanocrystals employed are commercially available and the developed reader was benchmarked with a standard portable quantum dot reader, thereby demonstrating advantages in terms of cost and linear analytical range. MDPI 2020-06-02 /pmc/articles/PMC7345677/ /pubmed/32498366 http://dx.doi.org/10.3390/bios10060060 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ireta-Muñoz, Laura Alejandra
Morales-Narváez, Eden
Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach
title Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach
title_full Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach
title_fullStr Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach
title_full_unstemmed Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach
title_short Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach
title_sort smartphone and paper-based fluorescence reader: a do it yourself approach
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345677/
https://www.ncbi.nlm.nih.gov/pubmed/32498366
http://dx.doi.org/10.3390/bios10060060
work_keys_str_mv AT iretamunozlauraalejandra smartphoneandpaperbasedfluorescencereaderadoityourselfapproach
AT moralesnarvaezeden smartphoneandpaperbasedfluorescencereaderadoityourselfapproach