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Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis

The Candida parapsilosis genome encodes for five agglutinin-like sequence (Als) cell-wall glycoproteins involved in adhesion to biotic and abiotic surfaces. The work presented here is aimed at analyzing the role of the two still uncharacterized ALS genes in C. parapsilosis, CpALS4790 and CpALS0660,...

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Autores principales: Zoppo, Marina, Fiorentini, Fabrizio, Rizzato, Cosmeri, Di Luca, Mariagrazia, Lupetti, Antonella, Bottai, Daria, Colone, Marisa, Stringaro, Annarita, De Bernardis, Flavia, Tavanti, Arianna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345767/
https://www.ncbi.nlm.nih.gov/pubmed/32545584
http://dx.doi.org/10.3390/jof6020086
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author Zoppo, Marina
Fiorentini, Fabrizio
Rizzato, Cosmeri
Di Luca, Mariagrazia
Lupetti, Antonella
Bottai, Daria
Colone, Marisa
Stringaro, Annarita
De Bernardis, Flavia
Tavanti, Arianna
author_facet Zoppo, Marina
Fiorentini, Fabrizio
Rizzato, Cosmeri
Di Luca, Mariagrazia
Lupetti, Antonella
Bottai, Daria
Colone, Marisa
Stringaro, Annarita
De Bernardis, Flavia
Tavanti, Arianna
author_sort Zoppo, Marina
collection PubMed
description The Candida parapsilosis genome encodes for five agglutinin-like sequence (Als) cell-wall glycoproteins involved in adhesion to biotic and abiotic surfaces. The work presented here is aimed at analyzing the role of the two still uncharacterized ALS genes in C. parapsilosis, CpALS4790 and CpALS0660, by the generation and characterization of CpALS4790 and CpALS066 single mutant strains. Phenotypic characterization showed that both mutant strains behaved as the parental wild type strain regarding growth rate in liquid/solid media supplemented with cell-wall perturbing agents, and in the ability to produce pseudohyphae. Interestingly, the ability of the CpALS0660 null mutant to adhere to human buccal epithelial cells (HBECs) was not altered when compared with the wild-type strain, whereas deletion of CpALS4790 led to a significant loss of the adhesion capability. RT-qPCR analysis performed on the mutant strains in co-incubation with HBECs did not highlight significant changes in the expression levels of others ALS genes. In vivo experiments in a murine model of vaginal candidiasis indicated a significant reduction in CFUs recovered from BALB/C mice infected with each mutant strain in comparison to those infected with the wild type strain, confirming the involvement of CpAls4790 and CpAls5600 proteins in C. parapsilosis vaginal candidiasis in mice.
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spelling pubmed-73457672020-07-09 Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis Zoppo, Marina Fiorentini, Fabrizio Rizzato, Cosmeri Di Luca, Mariagrazia Lupetti, Antonella Bottai, Daria Colone, Marisa Stringaro, Annarita De Bernardis, Flavia Tavanti, Arianna J Fungi (Basel) Article The Candida parapsilosis genome encodes for five agglutinin-like sequence (Als) cell-wall glycoproteins involved in adhesion to biotic and abiotic surfaces. The work presented here is aimed at analyzing the role of the two still uncharacterized ALS genes in C. parapsilosis, CpALS4790 and CpALS0660, by the generation and characterization of CpALS4790 and CpALS066 single mutant strains. Phenotypic characterization showed that both mutant strains behaved as the parental wild type strain regarding growth rate in liquid/solid media supplemented with cell-wall perturbing agents, and in the ability to produce pseudohyphae. Interestingly, the ability of the CpALS0660 null mutant to adhere to human buccal epithelial cells (HBECs) was not altered when compared with the wild-type strain, whereas deletion of CpALS4790 led to a significant loss of the adhesion capability. RT-qPCR analysis performed on the mutant strains in co-incubation with HBECs did not highlight significant changes in the expression levels of others ALS genes. In vivo experiments in a murine model of vaginal candidiasis indicated a significant reduction in CFUs recovered from BALB/C mice infected with each mutant strain in comparison to those infected with the wild type strain, confirming the involvement of CpAls4790 and CpAls5600 proteins in C. parapsilosis vaginal candidiasis in mice. MDPI 2020-06-12 /pmc/articles/PMC7345767/ /pubmed/32545584 http://dx.doi.org/10.3390/jof6020086 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zoppo, Marina
Fiorentini, Fabrizio
Rizzato, Cosmeri
Di Luca, Mariagrazia
Lupetti, Antonella
Bottai, Daria
Colone, Marisa
Stringaro, Annarita
De Bernardis, Flavia
Tavanti, Arianna
Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis
title Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis
title_full Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis
title_fullStr Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis
title_full_unstemmed Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis
title_short Role of CpALS4790 and CpALS0660 in Candida parapsilosis Virulence: Evidence from a Murine Model of Vaginal Candidiasis
title_sort role of cpals4790 and cpals0660 in candida parapsilosis virulence: evidence from a murine model of vaginal candidiasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345767/
https://www.ncbi.nlm.nih.gov/pubmed/32545584
http://dx.doi.org/10.3390/jof6020086
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