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Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography

An efficient strategy was developed for the rapid separation and enrichment of bafilomycin A1 (baf A1) from a crude extract of the marine microorganism Streptomyces lohii fermentation. This strategy comprises liquid−liquid extraction (LLE) with a three-phase solvent system (n-hexane–ethyl acetate–ac...

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Autores principales: Yuan, Ye, He, Xiaoping, Wang, Tingting, Zhang, Xingwang, Li, Zhong, Xu, Xiaoqing, Zhang, Weiyan, Yan, Xiaojun, Li, Shengying, He, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345786/
https://www.ncbi.nlm.nih.gov/pubmed/32630403
http://dx.doi.org/10.3390/md18060332
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author Yuan, Ye
He, Xiaoping
Wang, Tingting
Zhang, Xingwang
Li, Zhong
Xu, Xiaoqing
Zhang, Weiyan
Yan, Xiaojun
Li, Shengying
He, Shan
author_facet Yuan, Ye
He, Xiaoping
Wang, Tingting
Zhang, Xingwang
Li, Zhong
Xu, Xiaoqing
Zhang, Weiyan
Yan, Xiaojun
Li, Shengying
He, Shan
author_sort Yuan, Ye
collection PubMed
description An efficient strategy was developed for the rapid separation and enrichment of bafilomycin A1 (baf A1) from a crude extract of the marine microorganism Streptomyces lohii fermentation. This strategy comprises liquid−liquid extraction (LLE) with a three-phase solvent system (n-hexane–ethyl acetate–acetonitrile–water = 7:3:5:5, v/v/v/v) followed by separation using high-speed counter-current chromatography (HSCCC). The results showed that a 480.2-mg fraction of baf A1-enriched extract in the middle phase of the three-phase solvent system was prepared from 4.9 g of crude extract after two consecutive one-step operations. Over 99% of soybean oil, the main hydrophobic waste in the crude extract, and the majority of hydrophilic impurities were distributed in the upper and lower phase, respectively. HSCCC was used with a two-phase solvent system composed of n-hexane–acetonitrile–water (15:8:12, v/v/v) to isolate and purify baf A1 from the middle phase fraction, which yielded 77.4 mg of baf A1 with > 95% purity within 90 min. The overall recovery of baf A1 in the process was determined to be 95.7%. The use of a three-phase solvent system represents a novel strategy for the simultaneous removal of hydrophobic oil and hydrophilic impurities from a microbial fermentation extract.
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spelling pubmed-73457862020-07-09 Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography Yuan, Ye He, Xiaoping Wang, Tingting Zhang, Xingwang Li, Zhong Xu, Xiaoqing Zhang, Weiyan Yan, Xiaojun Li, Shengying He, Shan Mar Drugs Article An efficient strategy was developed for the rapid separation and enrichment of bafilomycin A1 (baf A1) from a crude extract of the marine microorganism Streptomyces lohii fermentation. This strategy comprises liquid−liquid extraction (LLE) with a three-phase solvent system (n-hexane–ethyl acetate–acetonitrile–water = 7:3:5:5, v/v/v/v) followed by separation using high-speed counter-current chromatography (HSCCC). The results showed that a 480.2-mg fraction of baf A1-enriched extract in the middle phase of the three-phase solvent system was prepared from 4.9 g of crude extract after two consecutive one-step operations. Over 99% of soybean oil, the main hydrophobic waste in the crude extract, and the majority of hydrophilic impurities were distributed in the upper and lower phase, respectively. HSCCC was used with a two-phase solvent system composed of n-hexane–acetonitrile–water (15:8:12, v/v/v) to isolate and purify baf A1 from the middle phase fraction, which yielded 77.4 mg of baf A1 with > 95% purity within 90 min. The overall recovery of baf A1 in the process was determined to be 95.7%. The use of a three-phase solvent system represents a novel strategy for the simultaneous removal of hydrophobic oil and hydrophilic impurities from a microbial fermentation extract. MDPI 2020-06-25 /pmc/articles/PMC7345786/ /pubmed/32630403 http://dx.doi.org/10.3390/md18060332 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuan, Ye
He, Xiaoping
Wang, Tingting
Zhang, Xingwang
Li, Zhong
Xu, Xiaoqing
Zhang, Weiyan
Yan, Xiaojun
Li, Shengying
He, Shan
Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography
title Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography
title_full Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography
title_fullStr Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography
title_full_unstemmed Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography
title_short Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography
title_sort efficient preparation of bafilomycin a1 from marine streptomyces lohii fermentation using three-phase extraction and high-speed counter-current chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345786/
https://www.ncbi.nlm.nih.gov/pubmed/32630403
http://dx.doi.org/10.3390/md18060332
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