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Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain

Streptomycetes are an important source of natural products potentially applicable in the pharmaceutical industry. Many of these drugs are secondary metabolites whose biosynthetic genes are very often poorly expressed under laboratory cultivation conditions. In many cases, antibiotic-resistant mutant...

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Autores principales: Rodríguez Estévez, Marta, Myronovskyi, Maksym, Rosenkränzer, Birgit, Paululat, Thomas, Petzke, Lutz, Ristau, Jeanette, Luzhetskyy, Andriy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345809/
https://www.ncbi.nlm.nih.gov/pubmed/32481766
http://dx.doi.org/10.3390/md18060284
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author Rodríguez Estévez, Marta
Myronovskyi, Maksym
Rosenkränzer, Birgit
Paululat, Thomas
Petzke, Lutz
Ristau, Jeanette
Luzhetskyy, Andriy
author_facet Rodríguez Estévez, Marta
Myronovskyi, Maksym
Rosenkränzer, Birgit
Paululat, Thomas
Petzke, Lutz
Ristau, Jeanette
Luzhetskyy, Andriy
author_sort Rodríguez Estévez, Marta
collection PubMed
description Streptomycetes are an important source of natural products potentially applicable in the pharmaceutical industry. Many of these drugs are secondary metabolites whose biosynthetic genes are very often poorly expressed under laboratory cultivation conditions. In many cases, antibiotic-resistant mutants exhibit increased production of natural drugs, which facilitates the identification and isolation of new substances. In this study, we report the induction of a type II polyketide synthase gene cluster in the marine strain Streptomyces albus subsp. chlorinus through the selection of streptomycin-resistant mutants, resulting in overproduction of the novel compound fredericamycin C(2) (1). Fredericamycin C(2) (1) is structurally related to the potent antitumor drug lead fredericamycin A.
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spelling pubmed-73458092020-07-09 Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain Rodríguez Estévez, Marta Myronovskyi, Maksym Rosenkränzer, Birgit Paululat, Thomas Petzke, Lutz Ristau, Jeanette Luzhetskyy, Andriy Mar Drugs Article Streptomycetes are an important source of natural products potentially applicable in the pharmaceutical industry. Many of these drugs are secondary metabolites whose biosynthetic genes are very often poorly expressed under laboratory cultivation conditions. In many cases, antibiotic-resistant mutants exhibit increased production of natural drugs, which facilitates the identification and isolation of new substances. In this study, we report the induction of a type II polyketide synthase gene cluster in the marine strain Streptomyces albus subsp. chlorinus through the selection of streptomycin-resistant mutants, resulting in overproduction of the novel compound fredericamycin C(2) (1). Fredericamycin C(2) (1) is structurally related to the potent antitumor drug lead fredericamycin A. MDPI 2020-05-28 /pmc/articles/PMC7345809/ /pubmed/32481766 http://dx.doi.org/10.3390/md18060284 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez Estévez, Marta
Myronovskyi, Maksym
Rosenkränzer, Birgit
Paululat, Thomas
Petzke, Lutz
Ristau, Jeanette
Luzhetskyy, Andriy
Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain
title Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain
title_full Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain
title_fullStr Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain
title_full_unstemmed Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain
title_short Novel Fredericamycin Variant Overproduced by a Streptomycin-Resistant Streptomyces albus subsp. chlorinus Strain
title_sort novel fredericamycin variant overproduced by a streptomycin-resistant streptomyces albus subsp. chlorinus strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345809/
https://www.ncbi.nlm.nih.gov/pubmed/32481766
http://dx.doi.org/10.3390/md18060284
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