Cargando…

The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential

Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Can...

Descripción completa

Detalles Bibliográficos
Autores principales: Milia, Egle, Usai, Marianna, Szotáková, Barbora, Elstnerová, Marie, Králová, Věra, D’hallewin, Guy, Spissu, Ylenia, Barberis, Antonio, Marchetti, Mauro, Bortone, Antonella, Campanella, Vincenzo, Mastandrea, Giorgio, Langhansová, Lenka, Eick, Sigrun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345856/
https://www.ncbi.nlm.nih.gov/pubmed/32466371
http://dx.doi.org/10.3390/antibiotics9060281
_version_ 1783556277850341376
author Milia, Egle
Usai, Marianna
Szotáková, Barbora
Elstnerová, Marie
Králová, Věra
D’hallewin, Guy
Spissu, Ylenia
Barberis, Antonio
Marchetti, Mauro
Bortone, Antonella
Campanella, Vincenzo
Mastandrea, Giorgio
Langhansová, Lenka
Eick, Sigrun
author_facet Milia, Egle
Usai, Marianna
Szotáková, Barbora
Elstnerová, Marie
Králová, Věra
D’hallewin, Guy
Spissu, Ylenia
Barberis, Antonio
Marchetti, Mauro
Bortone, Antonella
Campanella, Vincenzo
Mastandrea, Giorgio
Langhansová, Lenka
Eick, Sigrun
author_sort Milia, Egle
collection PubMed
description Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety. Methods: PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. Results: According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX-2 and LOX inflammatory enzymes, and without side effects against oral cells.
format Online
Article
Text
id pubmed-7345856
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73458562020-07-09 The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential Milia, Egle Usai, Marianna Szotáková, Barbora Elstnerová, Marie Králová, Věra D’hallewin, Guy Spissu, Ylenia Barberis, Antonio Marchetti, Mauro Bortone, Antonella Campanella, Vincenzo Mastandrea, Giorgio Langhansová, Lenka Eick, Sigrun Antibiotics (Basel) Article Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety. Methods: PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. Results: According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX-2 and LOX inflammatory enzymes, and without side effects against oral cells. MDPI 2020-05-26 /pmc/articles/PMC7345856/ /pubmed/32466371 http://dx.doi.org/10.3390/antibiotics9060281 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Milia, Egle
Usai, Marianna
Szotáková, Barbora
Elstnerová, Marie
Králová, Věra
D’hallewin, Guy
Spissu, Ylenia
Barberis, Antonio
Marchetti, Mauro
Bortone, Antonella
Campanella, Vincenzo
Mastandrea, Giorgio
Langhansová, Lenka
Eick, Sigrun
The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential
title The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential
title_full The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential
title_fullStr The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential
title_full_unstemmed The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential
title_short The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential
title_sort pharmaceutical ability of pistacia lentiscus l. leaves essential oil against periodontal bacteria and candida sp. and its anti-inflammatory potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345856/
https://www.ncbi.nlm.nih.gov/pubmed/32466371
http://dx.doi.org/10.3390/antibiotics9060281
work_keys_str_mv AT miliaegle thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT usaimarianna thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT szotakovabarbora thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT elstnerovamarie thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT kralovavera thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT dhallewinguy thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT spissuylenia thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT barberisantonio thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT marchettimauro thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT bortoneantonella thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT campanellavincenzo thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT mastandreagiorgio thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT langhansovalenka thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT eicksigrun thepharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT miliaegle pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT usaimarianna pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT szotakovabarbora pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT elstnerovamarie pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT kralovavera pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT dhallewinguy pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT spissuylenia pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT barberisantonio pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT marchettimauro pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT bortoneantonella pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT campanellavincenzo pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT mastandreagiorgio pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT langhansovalenka pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential
AT eicksigrun pharmaceuticalabilityofpistacialentiscuslleavesessentialoilagainstperiodontalbacteriaandcandidaspanditsantiinflammatorypotential