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The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential
Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Can...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345856/ https://www.ncbi.nlm.nih.gov/pubmed/32466371 http://dx.doi.org/10.3390/antibiotics9060281 |
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author | Milia, Egle Usai, Marianna Szotáková, Barbora Elstnerová, Marie Králová, Věra D’hallewin, Guy Spissu, Ylenia Barberis, Antonio Marchetti, Mauro Bortone, Antonella Campanella, Vincenzo Mastandrea, Giorgio Langhansová, Lenka Eick, Sigrun |
author_facet | Milia, Egle Usai, Marianna Szotáková, Barbora Elstnerová, Marie Králová, Věra D’hallewin, Guy Spissu, Ylenia Barberis, Antonio Marchetti, Mauro Bortone, Antonella Campanella, Vincenzo Mastandrea, Giorgio Langhansová, Lenka Eick, Sigrun |
author_sort | Milia, Egle |
collection | PubMed |
description | Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety. Methods: PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. Results: According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX-2 and LOX inflammatory enzymes, and without side effects against oral cells. |
format | Online Article Text |
id | pubmed-7345856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73458562020-07-09 The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential Milia, Egle Usai, Marianna Szotáková, Barbora Elstnerová, Marie Králová, Věra D’hallewin, Guy Spissu, Ylenia Barberis, Antonio Marchetti, Mauro Bortone, Antonella Campanella, Vincenzo Mastandrea, Giorgio Langhansová, Lenka Eick, Sigrun Antibiotics (Basel) Article Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety. Methods: PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. Results: According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX-2 and LOX inflammatory enzymes, and without side effects against oral cells. MDPI 2020-05-26 /pmc/articles/PMC7345856/ /pubmed/32466371 http://dx.doi.org/10.3390/antibiotics9060281 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Milia, Egle Usai, Marianna Szotáková, Barbora Elstnerová, Marie Králová, Věra D’hallewin, Guy Spissu, Ylenia Barberis, Antonio Marchetti, Mauro Bortone, Antonella Campanella, Vincenzo Mastandrea, Giorgio Langhansová, Lenka Eick, Sigrun The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential |
title | The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential |
title_full | The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential |
title_fullStr | The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential |
title_full_unstemmed | The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential |
title_short | The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential |
title_sort | pharmaceutical ability of pistacia lentiscus l. leaves essential oil against periodontal bacteria and candida sp. and its anti-inflammatory potential |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345856/ https://www.ncbi.nlm.nih.gov/pubmed/32466371 http://dx.doi.org/10.3390/antibiotics9060281 |
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