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Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion

This study experimentally analyses the binding characteristics of analytes mixed in liquid samples flowing along a micro-channel to the receptor fixed on the wall of the micro-channel to provide design tools and data for a microfluidic-based biosensor. The binding or detection characteristics are an...

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Autores principales: Choi, Sunghak, Lee, Woo Il, Lee, Gyu Hee, Yoo, Yeong-Eun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346006/
https://www.ncbi.nlm.nih.gov/pubmed/32503275
http://dx.doi.org/10.3390/mi11060570
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author Choi, Sunghak
Lee, Woo Il
Lee, Gyu Hee
Yoo, Yeong-Eun
author_facet Choi, Sunghak
Lee, Woo Il
Lee, Gyu Hee
Yoo, Yeong-Eun
author_sort Choi, Sunghak
collection PubMed
description This study experimentally analyses the binding characteristics of analytes mixed in liquid samples flowing along a micro-channel to the receptor fixed on the wall of the micro-channel to provide design tools and data for a microfluidic-based biosensor. The binding or detection characteristics are analyzed experimentally by counting the number of analytes bound to the receptor, with sample analyte concentration, sample flow rate, and the position of the receptor along the micro-channel length as the main variables. A mathematical model is also proposed to predict the number of analytes transported and bound to the receptor based on a probability density function for Brownian motion. The coefficient in the mathematical model is obtained by using a dimensionless mathematical model and the experimental results. The coefficient remains valid for all different conditions of the sample analyte concentration, flow rate, and the position of the receptor, which implies the possibility of deriving a generalized model. Based on the mathematical model derived from mathematical and experimental analysis on the detection characteristics of the microfluidic-based biosensor depending on previously mentioned variables and the height of the micro-channel, this study suggests a design for a microfluidic-based biosensor by predicting the binding efficiency according to the channel height. The results show the binding efficiency increases as the flow rate decreases and as the receptor is placed closer to the sample-injecting inlet, but is unaffected by sample concentration.
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spelling pubmed-73460062020-07-14 Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion Choi, Sunghak Lee, Woo Il Lee, Gyu Hee Yoo, Yeong-Eun Micromachines (Basel) Article This study experimentally analyses the binding characteristics of analytes mixed in liquid samples flowing along a micro-channel to the receptor fixed on the wall of the micro-channel to provide design tools and data for a microfluidic-based biosensor. The binding or detection characteristics are analyzed experimentally by counting the number of analytes bound to the receptor, with sample analyte concentration, sample flow rate, and the position of the receptor along the micro-channel length as the main variables. A mathematical model is also proposed to predict the number of analytes transported and bound to the receptor based on a probability density function for Brownian motion. The coefficient in the mathematical model is obtained by using a dimensionless mathematical model and the experimental results. The coefficient remains valid for all different conditions of the sample analyte concentration, flow rate, and the position of the receptor, which implies the possibility of deriving a generalized model. Based on the mathematical model derived from mathematical and experimental analysis on the detection characteristics of the microfluidic-based biosensor depending on previously mentioned variables and the height of the micro-channel, this study suggests a design for a microfluidic-based biosensor by predicting the binding efficiency according to the channel height. The results show the binding efficiency increases as the flow rate decreases and as the receptor is placed closer to the sample-injecting inlet, but is unaffected by sample concentration. MDPI 2020-06-03 /pmc/articles/PMC7346006/ /pubmed/32503275 http://dx.doi.org/10.3390/mi11060570 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Sunghak
Lee, Woo Il
Lee, Gyu Hee
Yoo, Yeong-Eun
Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion
title Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion
title_full Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion
title_fullStr Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion
title_full_unstemmed Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion
title_short Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion
title_sort analysis of the binding of analyte-receptor in a micro-fluidic channel for a biosensor based on brownian motion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346006/
https://www.ncbi.nlm.nih.gov/pubmed/32503275
http://dx.doi.org/10.3390/mi11060570
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